{"EMPIAR-13553":{"imagesets":[{"segmentations":[],"name":"Single-particle images of EF-G bound 70S ribosomes","directory":"data","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRC","num_images_or_tilt_series":1341961,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.04,"pixel_height":1.04,"micrographs_file_pattern":"","picked_particles_file_pattern":"data/particle_stack.mrc","picked_particles_directory":"data","details":"Particles were semiautomatically selected using e2boxer’s swarm tool, followed by manual curation of the dataset. Defocus parameters were determined using CTFFIND3. Boxing was performed using batchboxer, with particles stored in a single stack (particle_stack.mrc). frealign_parameters.par contains rough particle alignment parameters, obtained using FREALIGN, as well as other imaging-related parameters.","image_width":"320","image_height":"320"}],"workflow_file":null,"grant_references":[{"funding_body":"Natural Sciences and Engineering Research Council (NSERC, Canada)","code":"none","country":"Canada"},{"funding_body":"National Institutes of Health (NIH)","code":"GM-62580","country":"United States"},{"funding_body":"National Institutes of Health (NIH)","code":"GM-92424","country":"United States"},{"funding_body":"National Institutes of Health (NIH)","code":"GM-99719","country":"United States"}],"version_history":[],"title":"Structure of the ribosome with elongation factor G trapped in the pretranslocation state","principal_investigator":[{"author_orcid":"0000-0002-1506-909X","middle_name":null,"organization":"University of Massachusetts Chan Medical School","street":"368 Plantation Street, AS4-2049","town_or_city":"Worcester","state_or_province":"Massachusetts","post_or_zip":"01605","telephone":"+1 774 455 6682","fax":"+1 508 721 7027","first_name":"Nikolaus","last_name":"Grigorieff","email":"niko [at] grigorieff.org","country":"United States","entry":"EMPIAR-13553"},{"author_orcid":"0000-0002-7554-5967","middle_name":"N","organization":"Department of Biochemistry and Biophysics and Center for RNA Biology","street":"601 Elmwood Ave","town_or_city":"Rochester","state_or_province":"New York","post_or_zip":"14642","telephone":"+1 585 273 3321","fax":"+1 585 271 2683","first_name":"Dmitri","last_name":"Ermolenko","email":"dmitri_ermolenko [at] urmc.rochester.edu","country":"United States","entry":"EMPIAR-13553"},{"author_orcid":"0000-0003-1588-717X","middle_name":"A","organization":"University of Massachusetts Chan Medical School","street":"368 Plantation Street, AS5-1049","town_or_city":"Worcester","state_or_province":"Massachusetts","post_or_zip":"01605","telephone":"+1 508 856 2353","fax":"+1 508 856 6696","first_name":"Andrei","last_name":"Korostelev","email":"andrei.korostelev [at] umassmed.edu","country":"United States","entry":"EMPIAR-13553"}],"status":"REL","deposition_date":"2026-05-07","release_date":"2026-06-24","obsolete_date":null,"update_date":"2026-06-24","corresponding_author":{"author":{"author_orcid":"0000-0002-1506-909X","middle_name":null,"organization":"University of Massachusetts Chan Medical School","street":"368 Plantation Street, AS4-2049","town_or_city":"Worcester","state_or_province":"Massachusetts","post_or_zip":"01605","first_name":"Nikolaus","last_name":"Grigorieff","country":"United States"}},"authors":[{"author":{"name":"Brilot AF","author_orcid":"0000-0001-8548-4224"}},{"author":{"name":"Korostelev AA","author_orcid":"0000-0003-1588-717X"}},{"author":{"name":"Ermolenko DN","author_orcid":"0000-0002-7554-5967"}},{"author":{"name":"Grigorieff N","author_orcid":"0000-0002-1506-909X"}}],"cross_references":["EMD-5798","EMD-5800","EMD-5799","EMD-5796","EMD-5797"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Brilot AF","author_orcid":"0000-0001-8548-4224"},{"name":"Korostelev AA","author_orcid":"0000-0003-1588-717X"},{"name":"Ermolenko DN","author_orcid":"0000-0002-7554-5967"},{"name":"Grigorieff N","author_orcid":"0000-0002-1506-909X"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structure of the ribosome with elongation factor G trapped in the pretranslocation state","volume":"110","country":"United States","first_page":"20994","last_page":"20999","year":"2013","language":"English","doi":"10.1073/pnas.1311423110","pubmedid":"24324137","details":"Particle stack and Frealign parameters of EF-G-bound 70S ribosomes","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Proc Natl Acad Sci USA","journal_abbreviation":"PNAS","issue":"52","preprint":false}],"dataset_size":"512.1 GB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["4v7d","4v7c"],"entry_doi":"10.6019/EMPIAR-13553"}}