{"EMPIAR-13465":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame movies of RecF-RecO-RecR-DNA from Thermus thermophilus","directory":"data/Movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":7217,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.86,"pixel_height":0.86,"micrographs_file_pattern":"data/Movies/*.tiff","picked_particles_file_pattern":"data/EMD-*/EMD-*_particlestack.star","picked_particles_directory":"data/EMD-*","details":"Gain-corrected, unaligned multi-frame movies collected with Krios G3i microscope + K3 detector (superresolution mode, binned 2x with EPU). Voltage: 300 kV; spherical aberration: 2.7; dose per frame: ~1 e/A^2 (total dose: 41.71 e/A^2 fractionated over 40 frames). Defocus range (nominal): -2.7, -2.4, -2.1, -1.8, -1.5, -1.2, -0.9 µm. Objective aperture retracted, C2 aperture: 50 µm. See microscope_settings.txt for details and defectfile.txt for correcting camera defects during Motion correction in RELION.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Aligned particles of RecF-DNA from Thermus thermophilus","directory":"data/EMD-15231_particles","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":122950,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.86,"pixel_height":0.86,"micrographs_file_pattern":"data/Movies/*.tiff","picked_particles_file_pattern":"data/EMD-15231_particles/EMD-15231_particlestack.star","picked_particles_directory":"data/EMD-15231_particles","details":"Aligned particles with corresponding STAR file. Micrographs were initially binned 2x during Motion correction.","image_width":"326","image_height":"326"},{"segmentations":[],"name":"Aligned particles of RecF-RecR-DNA from Thermus thermophilus","directory":"data/EMD-15267_particles","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":318235,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.86,"pixel_height":0.86,"micrographs_file_pattern":"data/Movies/*.tiff","picked_particles_file_pattern":"data/EMD-15267_particles/EMD-15267_particlestack.star","picked_particles_directory":"data/EMD-15267_particles","details":"Aligned particles with corresponding STAR file. Micrographs were initially binned 2x during Motion correction.","image_width":"400","image_height":"400"},{"segmentations":[],"name":"Aligned particles of RecO-RecR-DNA from Thermus thermophilus","directory":"data/EMD-15308_particles","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":52241,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.86,"pixel_height":0.86,"micrographs_file_pattern":"data/Movies/*.tiff","picked_particles_file_pattern":"data/EMD-15308_particles/EMD-15308_particlestack.star","picked_particles_directory":"data/EMD-15308_particles","details":"Aligned particles with corresponding STAR file. Micrographs were initially binned 2x during Motion correction.","image_width":"280","image_height":"280"},{"segmentations":[],"name":"Aligned particles of RecF-RecO-RecR-DNA from Thermus thermophilus","directory":"data/EMD-16164_particles","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":52241,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.86,"pixel_height":0.86,"micrographs_file_pattern":"data/Movies/*.tiff","picked_particles_file_pattern":"data/EMD-16164_particles/EMD-16164_particlestack.star","picked_particles_directory":"data/EMD-16164_particles","details":"Aligned particles with corresponding STAR file. Micrographs were initially binned 2x during Motion correction.","image_width":"400","image_height":"400"}],"workflow_file":null,"grant_references":[{"funding_body":"Polish National Science Centre","code":"2017/26/A/NZ1/01098","country":"Poland"},{"funding_body":"Other government","code":"1/SOL/2021/2","country":"Poland"}],"version_history":[],"title":"Complex of RecF-RecO-RecR-DNA from Thermus thermophilus","principal_investigator":[{"author_orcid":"0000-0001-8632-0977","middle_name":null,"organization":"International Institute of Molecular and Cell Biology in Warsaw","street":"4 Ks. Trojdena Street","town_or_city":"Warsaw","state_or_province":null,"post_or_zip":"02-109","telephone":null,"fax":null,"first_name":"Marcin","last_name":"Nowotny","email":"mnowotny [at] iimcb.gov.pl","country":"Poland","entry":"EMPIAR-13465"}],"status":"REL","deposition_date":"2026-03-27","release_date":"2026-04-20","obsolete_date":null,"update_date":"2026-04-20","corresponding_author":{"author":{"author_orcid":"0000-0001-5582-2851","middle_name":null,"organization":"International Institute of Molecular and Cell Biology in Warsaw","street":"4 Ks. Trojdena Street","town_or_city":"Warsaw","state_or_province":null,"post_or_zip":"02-106","first_name":"Mariusz","last_name":"Czarnocki-Cieciura","country":"Poland"}},"authors":[{"author":{"name":"Nirwal S","author_orcid":"0000-0002-4956-0696"}},{"author":{"name":"Czarnocki-Cieciura M","author_orcid":"0000-0001-5582-2851"}},{"author":{"name":"Chaudhary A","author_orcid":"0000-0003-3614-3698"}},{"author":{"name":"Zajko W","author_orcid":"0000-0001-9915-0305"}},{"author":{"name":"Skowronek K","author_orcid":"0000-0001-9973-7085"}},{"author":{"name":"Chamera S","author_orcid":"0000-0002-2328-9703"}},{"author":{"name":"Figiel M","author_orcid":"0000-0002-2211-0425"}},{"author":{"name":"Nowotny M","author_orcid":"0000-0001-8632-0977"}}],"cross_references":["EMD-15308","EMD-16164","EMD-15267","EMD-15231"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Nirwal S","author_orcid":"0000-0002-4956-0696"},{"name":"Czarnocki-Cieciura M","author_orcid":"0000-0001-5582-2851"},{"name":"Chaudhary A","author_orcid":"0000-0003-3614-3698"},{"name":"Zajko W","author_orcid":"0000-0001-9915-0305"},{"name":"Skowronek K","author_orcid":"0000-0001-9973-7085"},{"name":"Chamera S","author_orcid":"0000-0002-2328-9703"},{"name":"Figiel M","author_orcid":"0000-0002-2211-0425"},{"name":"Nowotny M","author_orcid":"0000-0001-8632-0977"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Mechanism of RecF-RecO-RecR cooperation in bacterial homologous recombination","volume":"30","country":"","first_page":"650","last_page":"660","year":"2023","language":"English","doi":"10.1038/s41594-023-00967-z","pubmedid":"37081315","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature structural & molecular biology","journal_abbreviation":"Nat Struct Mol Biol","issue":"5","preprint":false}],"dataset_size":"3.2 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["8ab0","8bpr","8a93","8a8j"],"entry_doi":"10.6019/EMPIAR-13465"}}