{"EMPIAR-13435":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of Azotobacter vinelandii MoFeP determined using the SPT Labtech chameleon in the presence of 0x SurfACT.","directory":"data/movies","category":"micrographs - multiframe","header_format":"EER","data_format":"EER","num_images_or_tilt_series":968,"frames_per_image":1386,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.735,"pixel_height":0.735,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned multi-frame micrographs of Azotobacter vinelandii MoFeP determined using the SPT Labtech chameleon in the presence of 0x SurfACT collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2.","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"Aligned micrographs of Azotobacter vinelandii MoFeP determined using the SPT Labtech chameleon in the presence of 0x SurfACT.","directory":"data/micrographs","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":968,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.735,"pixel_height":0.735,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Aligned micrographs of Azotobacter vinelandii MoFeP determined using the SPT Labtech chameleon in the presence of 0x SurfACT collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon4 direct electron detector. Micrographs were aligned in cryoSPARC.","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"Gain file required for motion correction of unaligned micrographs of Azotobacter vinelandii MoFeP determined using the SPT Labtech chameleon in the presence of 0x SurfACT.","directory":"data/gain","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.735,"pixel_height":0.735,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Gain file required for motion correction of unaligned micrographs of Azotobacter vinelandii MoFeP determined using the SPT Labtech chameleon in the presence of 0x SurfACT.","image_width":"4096","image_height":"4096"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)","code":"5R35GM138206","country":"United States"},{"funding_body":"National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)","code":"1S10OD032471","country":"United States"},{"funding_body":"Searle Scholars Program","code":"Searle Scholars Program","country":"United States"},{"funding_body":"Other private","code":"Cottrell Scholars","country":"United States"},{"funding_body":"Other private","code":"George W. and Carol A. Lattimer Faculty Research Fellowship","country":"United States"}],"version_history":[],"title":"CryoEM movies of Azotobacter vinelandii MoFeP determined using the SPT Labtech chameleon in the presence of 0x SurfACT.","principal_investigator":[{"author_orcid":"0000-0001-6653-6682","middle_name":"A","organization":"Department of Chemistry and Biochemistry","street":"9500 Gilman Drive, Natural Sciences Building 4105","town_or_city":"La Jolla","state_or_province":"California","post_or_zip":"92093","telephone":null,"fax":null,"first_name":"Mark","last_name":"Herzik","email":"mherzik [at] ucsd.edu","country":"United States","entry":"EMPIAR-13435"}],"status":"REL","deposition_date":"2026-03-27","release_date":"2026-04-15","obsolete_date":null,"update_date":"2026-04-15","corresponding_author":{"author":{"author_orcid":"0000-0001-6653-6682","middle_name":"A","organization":"Department of Chemistry and Biochemistry","street":"9500 Gilman Drive, Natural Sciences Building 4105","town_or_city":"La Jolla","state_or_province":"California","post_or_zip":"92093","first_name":"Mark","last_name":"Herzik","country":"United States"}},"authors":[{"author":{"name":"Enos SE","author_orcid":"0000-0001-8761-7457"}},{"author":{"name":"Cook BD","author_orcid":"0000-0002-5777-3464"}},{"author":{"name":"Rahmani H","author_orcid":"0000-0003-0222-6989"}},{"author":{"name":"Narehood SM","author_orcid":"0000-0001-6822-8608"}},{"author":{"name":"Li Y","author_orcid":"0009-0000-5502-4243"}},{"author":{"name":"Kuschnerus IC","author_orcid":"0000-0002-4453-6849"}},{"author":{"name":"Redford HT","author_orcid":"0009-0001-6633-5976"}},{"author":{"name":"Dukakis P","author_orcid":"0009-0003-0253-8090"}},{"author":{"name":"Ji D","author_orcid":"0009-0003-2008-9583"}},{"author":{"name":"Bachochin MJ","author_orcid":"0000-0002-0989-1538"}},{"author":{"name":"Grotjahn DA","author_orcid":"0000-0001-5908-7882"}},{"author":{"name":"Herzik MA","author_orcid":"0000-0001-6653-6682"}}],"cross_references":["EMD-75854","EMD-75855"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Enos SE","author_orcid":"0000-0001-8761-7457"},{"name":"Cook BD","author_orcid":"0000-0002-5777-3464"},{"name":"Rahmani H","author_orcid":"0000-0003-0222-6989"},{"name":"Narehood SM","author_orcid":"0000-0001-6822-8608"},{"name":"Li Y","author_orcid":"0009-0000-5502-4243"},{"name":"Kuschnerus IC","author_orcid":"0000-0002-4453-6849"},{"name":"Redford HT","author_orcid":"0009-0001-6633-5976"},{"name":"Dukakis P","author_orcid":"0009-0003-0253-8090"},{"name":"Ji D","author_orcid":"0009-0003-2008-9583"},{"name":"Bachochin MJ","author_orcid":"0000-0002-0989-1538"},{"name":"Grotjahn DA","author_orcid":"0000-0001-5908-7882"},{"name":"Herzik MA","author_orcid":"0000-0001-6653-6682"}],"editors":[],"published":false,"j_or_nj_citation":true,"title":"A Surfactant Cocktail Overcomes Air-Water Interface Artifacts in Single-Particle CryoEM","volume":null,"country":"","first_page":null,"last_page":null,"year":null,"language":null,"doi":"10.64898/2026.03.17.712260","pubmedid":null,"details":"CryoEM movies of Azotobacter vinelandii MoFeP determined using the SPT Labtech chameleon in the presence of 0x SurfACT. Data were collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2 with a defocus range of −0.75 to −2.5 µm. Data were collected automatically using EPU with aberration free image shift.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"","journal_abbreviation":"","issue":null,"preprint":true}],"dataset_size":"635.2 GB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["11na","11nb"],"entry_doi":"10.6019/EMPIAR-13435"}}