{"EMPIAR-13396":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame movies of C. thermocellum UvrA in complex with DNA with an abasic site and AMPPNP (untilted movies)","directory":"data/Movies/230629_untilted","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":6678,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.84,"pixel_height":0.84,"micrographs_file_pattern":"data/Movies","picked_particles_file_pattern":"data/EMD-5167*_particles/*.star","picked_particles_directory":"data/EMD-5167*_particles","details":"Gain-corrected, unaligned multi-frame movies collected with Krios G3i microscope + K3 detector (superresolution mode, binned 2x with EPU) with 0 deg stage tilt. Voltage: 300 kV; spherical aberration: 2.7; dose per frame: ~1 e/A^2 (total dose: 40.64 e/A^2 fractionated over 40 frames). Defocus range (nominal): -2.1, -1.8, -1.5, -1.2, -0.9 µm. Objective aperture retracted, C2 aperture: 50 µm. See microscope_settings.txt for details","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Unaligned multi-frame movies of C. thermocellum UvrA in complex with DNA with an abasic site and AMPPNP (tilted movies)","directory":"data/Movies/230921_tilted","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":2994,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.84,"pixel_height":0.84,"micrographs_file_pattern":"data/Movies","picked_particles_file_pattern":"data/EMD-5167*_particles/*.star","picked_particles_directory":"data/EMD-5167*_particles","details":"Gain-corrected, unaligned multi-frame movies collected with Krios G3i microscope + K3 detector (superresolution mode, binned 2x with EPU) with 30 deg stage tilt. Voltage: 300 kV; spherical aberration: 2.7; dose per frame: ~1 e/A^2 (total dose: 40.49 e/A^2 fractionated over 40 frames). Defocus range (nominal): -2.0, -1.5, -1.0 µm. Objective aperture retracted, C2 aperture: 50 µm. See microscope_settings.txt for details","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Aligned particles of C. thermocellum UvrA in complex with DNA with an abasic site and AMPPNP (ATP-bound conformation 1)","directory":"data/EMD-51671_particles","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":197058,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.84,"pixel_height":0.84,"micrographs_file_pattern":"data/Movies","picked_particles_file_pattern":"data/EMD-51671_particles/EMD-51671_particlestack.star","picked_particles_directory":"data/EMD-51671_particles","details":"Aligned particles with corresponding STAR file. Micrographs were initially binned 2x during Motion correction.","image_width":"320","image_height":"320"},{"segmentations":[],"name":"Aligned particles of C. thermocellum UvrA in complex with DNA with an abasic site and AMPPNP (ATP-bound conformation 2)","directory":"data/EMD-51672_particles","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":195233,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.84,"pixel_height":0.84,"micrographs_file_pattern":"data/Movies","picked_particles_file_pattern":"data/EMD-51672_particles/EMD-51672_particlestack.star","picked_particles_directory":"data/EMD-51672_particles","details":"Aligned particles with corresponding STAR file. Micrographs were initially binned 2x during Motion correction.","image_width":"320","image_height":"320"}],"workflow_file":null,"grant_references":[{"funding_body":"Polish National Science Centre","code":"2017/ 26/A/NZ1/01098","country":"Poland"},{"funding_body":"Other government","code":"1/SOL/2021/2","country":"Poland"},{"funding_body":"European Union (EU)","code":"101059801 - RACE","country":"Poland"},{"funding_body":"European Union (EU)","code":"IN-MOL-CELL Infrastructure","country":"Poland"}],"version_history":[],"title":"C. thermocellum UvrA in complex with DNA with an abasic site and AMPPNP","principal_investigator":[{"author_orcid":"0000-0001-8632-0977","middle_name":null,"organization":"International Institute of Molecular and Cell Biology in Warsaw","street":"4 Ks. Trojdena Street","town_or_city":"Warsaw","state_or_province":null,"post_or_zip":"02-109","telephone":null,"fax":null,"first_name":"Marcin","last_name":"Nowotny","email":"mnowotny [at] iimcb.gov.pl","country":"Poland","entry":"EMPIAR-13396"}],"status":"REL","deposition_date":"2026-03-14","release_date":"2026-03-27","obsolete_date":null,"update_date":"2026-03-27","corresponding_author":{"author":{"author_orcid":"0000-0001-5582-2851","middle_name":null,"organization":"International Institute of Molecular and Cell Biology in Warsaw","street":"4 Ks. Trojdena Street","town_or_city":"Warsaw","state_or_province":null,"post_or_zip":"02-106","first_name":"Mariusz","last_name":"Czarnocki-Cieciura","country":"Poland"}},"authors":[{"author":{"name":"Nirwal S","author_orcid":"0000-0002-4956-0696"}},{"author":{"name":"Czarnocki-Cieciura M","author_orcid":"0000-0001-5582-2851"}},{"author":{"name":"Zajko W","author_orcid":"0000-0001-9915-0305"}},{"author":{"name":"Skowronek K","author_orcid":"0000-0001-9973-7085"}},{"author":{"name":"Szczepanowski RH","author_orcid":"0009-0009-8841-7903"}},{"author":{"name":"Nowotny M","author_orcid":"0000-0001-8632-0977"}}],"cross_references":["EMD-51672","EMD-51671"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Nirwal S","author_orcid":"0000-0002-4956-0696"},{"name":"Czarnocki-Cieciura M","author_orcid":"0000-0001-5582-2851"},{"name":"Zajko W","author_orcid":"0000-0001-9915-0305"},{"name":"Skowronek K","author_orcid":"0000-0001-9973-7085"},{"name":"Szczepanowski RH","author_orcid":"0009-0009-8841-7903"},{"name":"Nowotny M","author_orcid":"0000-0001-8632-0977"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structural snapshots of the mechanism of ATP-dependent DNA damage recognition by UvrA","volume":"17","country":"","first_page":null,"last_page":null,"year":"2025","language":"English","doi":"10.1038/s41467-025-67075-y","pubmedid":"41381534","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature communications","journal_abbreviation":"Nat Commun","issue":"1","preprint":false}],"dataset_size":"2.7 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9gxr","9gxq"],"entry_doi":"10.6019/EMPIAR-13396"}}