{"EMPIAR-13391":{"imagesets":[{"segmentations":[],"name":"Tilt-series on lamellae of Dictyostelium discoideum cells acquired with a 1-degree tilt increment","directory":"data/Data_1degree","category":"tilt series","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":63,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":1.971,"pixel_height":1.971,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Tilt-series were aimed to collect 121 tilts, for some, fewer tilts were acquired. Tilt-series are sorted according to ascending tilt angle apart from -60 to +60 from lamella pre-tilt (8deg). mdoc files with metadata provided by SerialEM are also provided.\nTilt images that were discarded during processing are specified in \"tilt_clean_1deg.txt\".\nAlignment results from IMOD are provided as TS_XXXX.xf files.\nTomograms that were used for STA are marked in tomo_list_1degree.txt.\nCoordinates to raw ribosome particle picks by template matching are provided in TM_1degree_threshold5.star and TM_1degree_threshold6.star. Ribosome particle locations after M refinment are provided in M_1degree_particles.star.","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"Tilt-series on lamellae of Dictyostelium discoideum cells acquired with a 2-degree tilt increment","directory":"data/Data_2degree","category":"tilt series","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":119,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":1.971,"pixel_height":1.971,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Tilt-series were aimed to collect 61 tilts, for some, fewer tilts were acquired. Tilt-series are sorted according to ascending tilt angle apart from -60 to +60 from lamella pre-tilt (8deg). mdoc files with metadata provided by SerialEM are also provided.\nTilt images that were discarded during processing are specified in \"tilt_clean_2deg.txt\".\nAlignment results from IMOD are provided as TS_XXXX.xf files.\nTomograms that were used for STA are marked in tomo_list_2degree.txt.\nCoordinates to raw ribosome particle picks by template matching are provided in TM_2degree_threshold5.star and TM_2degree_threshold6.star. Ribosome particle locations after M refinment are provided in M_2degree_particles.star.","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"Tilt-series on lamellae of Dictyostelium discoideum cells acquired with a 3-degree tilt increment","directory":"data/Data_3degree","category":"tilt series","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":120,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":1.971,"pixel_height":1.971,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Tilt-series were aimed to collect 41 tilts, for some, fewer tilts were acquired. Tilt-series are sorted according to ascending tilt angle apart from -60 to +60 from lamella pre-tilt (8deg). mdoc files with metadata provided by SerialEM are also provided.\nTilt images that were discarded during processing are specified in \"tilt_clean_3deg.txt\".\nAlignment results from IMOD are provided as TS_XXXX.xf files.\nTomograms that were used for STA are marked in tomo_list_3degree.txt.\nCoordinates to raw ribosome particle picks by template matching are provided in TM_3degree_threshold5.star and TM_3degree_threshold5.5.star. Ribosome particle locations after M refinment are provided in M_3degree_particles.star.","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"Tilt-series on lamellae of Dictyostelium discoideum cells acquired with a 5-degree tilt increment","directory":"data/Data_5degree","category":"tilt series","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":122,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":1.971,"pixel_height":1.971,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Tilt-series were aimed to collect 25 tilts, for some, fewer tilts were acquired. Tilt-series are sorted according to ascending tilt angle apart from -60 to +60 from lamella pre-tilt (8deg). mdoc files with metadata provided by SerialEM are also provided.\nTilt images that were discarded during processing are specified in \"tilt_clean_5deg.txt\".\nAlignment results from IMOD are provided as TS_XXXX.xf files.\nTomograms that were used for STA are marked in tomo_list_5degree.txt.\nCoordinates to raw ribosome particle picks by template matching are provided in TM_5degree_threshold5.star. Ribosome particle locations after M refinment are provided in M_5degree_particles.star.","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"Tilt-series on lamellae of Dictyostelium discoideum cells acquired with a 10-degree tilt increment","directory":"data/Data_10degree","category":"tilt series","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":89,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":1.971,"pixel_height":1.971,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Tilt-series were aimed to collect 13 tilts, for some, fewer tilts were acquired. Tilt-series are sorted according to ascending tilt angle apart from -60 to +60 from lamella pre-tilt (8deg). mdoc files with metadata provided by SerialEM are also provided.\nTilt images that were discarded during processing are specified in \"tilt_clean_10deg.txt\".\nAlignment results from IMOD are provided as TS_XXXX.xf files.\nTomograms that were used for STA are marked in tomo_list_10degree.txt.\nCoordinates to raw ribosome particle picks by template matching are provided in TM_10degree_threshold3.75.star. Ribosome particle locations after M refinment are provided in M_10degree_particles.star.","image_width":"4096","image_height":"4096"}],"workflow_file":null,"grant_references":[{"funding_body":"Chan Zuckerberg Initiative","code":"2021-234666","country":"United States"}],"version_history":[],"title":"Cryo-ET dataset on lamellae of Dictyostelium discoideum cells acquired with various tilt-increments","principal_investigator":[{"author_orcid":"0000-0002-7397-1321","middle_name":null,"organization":"Max Planck Institute of Biophysics, Department of Molecular Sociology","street":"Max-von-Laue-Strasse 3","town_or_city":"Frankfurt am Main","state_or_province":"Hessen","post_or_zip":"60438","telephone":null,"fax":null,"first_name":"Martin","last_name":"Beck","email":"martin.beck [at] biophys.mpg.de","country":"Germany","entry":"EMPIAR-13391"}],"status":"REL","deposition_date":"2026-02-27","release_date":"2026-07-27","obsolete_date":null,"update_date":"2026-07-27","corresponding_author":{"author":{"author_orcid":"0000-0002-7397-1321","middle_name":null,"organization":"Max Planck Institute of Biophysics","street":"Max-von-Laue-Strasse 3","town_or_city":"Frankfurt am Main","state_or_province":"Hessen","post_or_zip":"60438","first_name":"Martin","last_name":"Beck","country":"Germany"}},"authors":[{"author":{"name":"Tuijtel MW","author_orcid":"0000-0002-0615-572X"}},{"author":{"name":"Beck M","author_orcid":"0000-0002-7397-1321"}}],"cross_references":[],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Tuijtel MW","author_orcid":"0000-0002-0615-572X"},{"name":"Majtner M","author_orcid":"0000-0002-5279-8806"},{"name":"Turoňová B","author_orcid":"0000-0002-5457-4478"},{"name":"Beck M","author_orcid":"0000-0002-7397-1321"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Optimising the tilt-increment for in situ cryo-electron tomography","volume":null,"country":"","first_page":null,"last_page":null,"year":null,"language":"English","doi":"10.7554/eLife.111639.1","pubmedid":null,"details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"eLife","journal_abbreviation":"","issue":null,"preprint":false}],"dataset_size":"707.5 GB","experiment_type":"EMDB","scale":"cell","entry_doi":"10.6019/EMPIAR-13391"}}