{"EMPIAR-13389":{"imagesets":[{"segmentations":[],"name":"Raw, unaligned cryo-EM micrographs of phosphorylated AP-2","directory":"data/pAP2","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":542,"frames_per_image":60,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.58,"pixel_height":0.58,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Raw, unaligned cryo-EM movies of phosphorylated AP-2 (pAP2). Movies were collected on a Talos Arctica cryoTEM with a Gatan K2 camera operating in super-resolution mode.\n\n200 keV Talos Arctica cryoTEM\nImages were collected at 36,000x, yielding a final pixel size of 1.16 Å for counting mode and 0.58 Å for super resolution mode\nexposure rate of ~6 e-/pixel/s with 200 ms frames and a total exposure of ~50 e-/Å2\nCs - 2.7 mm\nAc - 0.1","image_width":"7676","image_height":"7420"},{"segmentations":[],"name":"Raw, unaligned cryo-EM micrographs of phosphorylated AP-2 bound to NECAP","directory":"data/pAP2+NECAP","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":2860,"frames_per_image":60,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.58,"pixel_height":0.58,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Raw, unaligned cryo-EM movies of phosphorylated AP-2 (pAP2) bound to the endocytic regulator NECAP. Movies were collected on a Talos Arctica cryoTEM with a Gatan K2 camera operating in super-resolution mode.\n\n200 keV Talos Arctica cryoTEM\nImages were collected at 36,000x, yielding a final pixel size of 1.16 Å for counting mode and 0.58 Å for super resolution mode\nexposure rate of ~6 e-/pixel/s with 200 ms frames and a total exposure of ~50 e-/Å2\nCs - 2.7 mm\nAc - 0.1","image_width":"7676","image_height":"7420"},{"segmentations":[],"name":"Raw, unaligned cryo-EM micrographs of phosphorylated AP-2 bound to NECAP in the presence of DNA","directory":"data/pAP2+NECAP+DNA","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":2314,"frames_per_image":60,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":1.16,"pixel_height":1.16,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Raw, unaligned cryo-EM movies of phosphorylated AP-2 (pAP2) bound to the endocytic regulator NECAP in the presence of DNA. Movies were collected on a Talos Arctica cryoTEM with a Gatan K2 camera operating in counting mode.\n\n200 keV Talos Arctica cryoTEM\nImages were collected at 36,000x, yielding a final pixel size of 1.16 Å for counting mode and 0.58 Å for super resolution mode\nexposure rate of ~6 e-/pixel/s with 200 ms frames and a total exposure of ~50 e-/Å2\nCs - 2.7 mm\nAc - 0.1","image_width":"3838","image_height":"3710"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)","code":"R01 GM127548","country":"United States"},{"funding_body":"National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)","code":"R01 GM127548-01A1","country":"United States"},{"funding_body":"Damon Runyon Cancer Research Foundation","code":"DRG-#2285-17/","country":"United States"},{"funding_body":"National Institutes of Health (NIH)","code":"S10 OD018516","country":"United States"}],"version_history":[],"title":"Cryo-EM micrographs of phosphorylated AP2 bound to the endocytic regulator NECAP","principal_investigator":[{"author_orcid":"0000-0003-1136-6000","middle_name":null,"organization":"Cornell University","street":null,"town_or_city":"Ithaca","state_or_province":"NY","post_or_zip":"14853","telephone":"8569791403","fax":null,"first_name":"Gunther","last_name":"Hollopeter","email":"gh383 [at] cornell.edu","country":"United States","entry":"EMPIAR-13389"}],"status":"REL","deposition_date":"2026-03-18","release_date":"2026-04-08","obsolete_date":null,"update_date":"2026-04-08","corresponding_author":{"author":{"author_orcid":"0000-0003-1136-6000","middle_name":"Wayne","organization":"University of North Carolina at Chapel Hill","street":"3049E Genetic Medicine Bldg","town_or_city":"Chapel Hill","state_or_province":"NC","post_or_zip":"27599","first_name":"Richard","last_name":"Baker","country":"United States"}},"authors":[{"author":{"name":"Partlow EA","author_orcid":"0000-0001-5513-088X"}},{"author":{"name":"Baker RW","author_orcid":"0000-0003-1136-6000"}},{"author":{"name":"Leschziner AE","author_orcid":"0000-0002-7732-7023"}},{"author":{"name":"Hollopeter G","author_orcid":"0000-0002-6409-0530"}}],"cross_references":["EMD-20220","EMD-20215"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Partlow EA","author_orcid":"0000-0001-5513-088X"},{"name":"Baker RW","author_orcid":"0000-0003-1136-6000"},{"name":"Leschziner AE","author_orcid":"0000-0002-7732-7023"},{"name":"Hollopeter G","author_orcid":"0000-0002-6409-0530"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"A structural mechanism for phosphorylation-dependent inactivation of the AP2 complex","volume":"8","country":"","first_page":null,"last_page":null,"year":"2019","language":"English","doi":"10.7554/elife.50003","pubmedid":"31464684","details":"Raw, unaligned movies of phosphorylated AP2 in three distinct states. \nPhosphorylated AP2\nPhosphorylated AP2 bound to NECAP\nPhosphorylated AP2 bound to NECAP and DNA","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"eLife","journal_abbreviation":"Elife","issue":null,"preprint":false}],"dataset_size":"2.6 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["6oxl","6owo"],"entry_doi":"10.6019/EMPIAR-13389"}}