{"EMPIAR-13388":{"imagesets":[{"segmentations":[],"name":"Raw, aligned cryo-EM micrographs of the clathrin adaptor complex AP-2 bound to CCDC32 - dataset 1","directory":"data/dataset1","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":31870,"frames_per_image":60,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.657,"pixel_height":0.657,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"This deposition contains two datasets, collected on the same microscope with the same settings. The datasets come from two grids made during the same freezing session, differing only in the amount of time the proteins were allowed to incubate before freezing (dataset 1 = short incubation; dataset 2 = long incubation).\n\nAligned, dose-weighted micrographs from cryoSPARC v4.7.0 are also included for each dataset.\n\nThe dataset contains at least three distinct assemblies: AP-2 in the closed conformation; AP-2 bound to CCDC32 in a \"bowl\" conformation; and AP-2 bound to CCDC32 in the \"cargo-like\" conformation. The datasets only differ in the relative proportion of these three assemblies.\n\nkeV - 300\nCs - 2.7\nAc - 0.1\nAngpix = 0.657\nFrames - 60\nTotal dose - 60 e-/A2","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Raw, aligned cryo-EM micrographs of the clathrin adaptor complex AP-2 bound to CCDC32 - dataset 2","directory":"data/dataset2","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":36778,"frames_per_image":60,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.657,"pixel_height":0.657,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"This deposition contains two datasets, collected on the same microscope with the same settings. The datasets come from two grids made during the same freezing session, differing only in the amount of time the proteins were allowed to incubate before freezing (dataset 1 = short incubation; dataset 2 = long incubation).\n\nAligned, dose-weighted micrographs from cryoSPARC v4.7.0 are also included for each dataset.\n\nThe dataset contains at least three distinct assemblies: AP-2 in the closed conformation; AP-2 bound to CCDC32 in a \"bowl\" conformation; and AP-2 bound to CCDC32 in the \"cargo-like\" conformation. The datasets only differ in the relative proportion of these three assemblies.\n\nkeV - 300\nCs - 2.7\nAc - 0.1\nAngpix = 0.657\nFrames - 60\nTotal dose - 60 e-/A2","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)","code":"R35 GM150960","country":"United States"},{"funding_body":"National Institutes of Health/National Institute of Dental and Craniofacial Research (NIH/NIDCR)","code":"F31DE034311","country":"United States"},{"funding_body":"Japan Society for the Promotion of Science (JSPS)","code":"SP24007","country":"Japan"},{"funding_body":"Japan Society for the Promotion of Science (JSPS)","code":"21H04762","country":"Japan"},{"funding_body":"Japan Society for the Promotion of Science (JSPS)","code":"21H05248","country":"Japan"},{"funding_body":"Japan Agency for Medical Research and Development (AMED)","code":"JP24ama121002","country":"Japan"}],"version_history":[],"title":"Cryo-EM micrographs of the clathrin adaptor complex AP-2 and the assembly chaperone CCDC32; dataset contains multiple assembly and conformational states","principal_investigator":[{"author_orcid":"0000-0003-1136-6000","middle_name":"Wayne","organization":"University of North Carolina at Chapel Hill","street":"3049E Genetic Medicine Bldg","town_or_city":"Chapel Hill","state_or_province":"NC","post_or_zip":"27599","telephone":"8569791403","fax":null,"first_name":"Richard","last_name":"Baker","email":"baker [at] med.unc.edu","country":"United States","entry":"EMPIAR-13388"}],"status":"REL","deposition_date":"2026-03-13","release_date":"2026-05-02","obsolete_date":null,"update_date":"2026-05-02","corresponding_author":{"author":{"author_orcid":"0000-0003-1136-6000","middle_name":"Wayne","organization":"University of North Carolina at Chapel Hill","street":"3049E Genetic Medicine Bldg","town_or_city":"Chapel Hill","state_or_province":"NC","post_or_zip":"27599","first_name":"Richard","last_name":"Baker","country":"United States"}},"authors":[{"author":{"name":"Sloan DE","author_orcid":"0000-0001-6065-176X"}},{"author":{"name":"Yanagisawa H","author_orcid":"0000-0003-0313-2343"}},{"author":{"name":"Kikkawa M","author_orcid":"0000-0001-7656-8194"}},{"author":{"name":"Baker RW","author_orcid":"0000-0003-1136-6000"}}],"cross_references":["EMD-71907","EMD-71913","EMD-71905","EMD-71912","EMD-71909","EMD-71914","EMD-71910","EMD-71906","EMD-71911"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Sloan DE","author_orcid":"0000-0001-6065-176X"},{"name":"Yanagisawa H","author_orcid":"0000-0003-0313-2343"},{"name":"Kikkawa M","author_orcid":"0000-0001-7656-8194"},{"name":"Baker RW","author_orcid":"0000-0003-1136-6000"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"CCDC32 collaborates with the membrane to assemble the AP-2 clathrin adaptor complex","volume":null,"country":"","first_page":null,"last_page":null,"year":"2025","language":null,"doi":"10.1101/2025.08.05.668722","pubmedid":"40799577","details":"Cryo-EM micrographs of the clathrin adaptor complex AP-2 and the assembly chaperone CCDC32; dataset contains multiple assembly and conformational states","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"bioRxiv","journal_abbreviation":"","issue":null,"preprint":true}],"dataset_size":"17.7 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9pwa","9pwb","9pwc"],"entry_doi":"10.6019/EMPIAR-13388"}}