{"EMPIAR-13387":{"imagesets":[{"segmentations":[{"file":"data/PbSFA_Tomo/Figure8A/Segmentation_WT_initiation.mod","description":"Both tomogram reconstruction and segmentation were performed with IMOD","original_files":null,"original_format":"MOD","entry":null}],"name":"Electron tomograms of WT budding sporozoites at initiation stage","directory":"data/PbSFA_Tomo/Figure8A","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.9058,"pixel_height":1.9058,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The tomogram used to generate figure8A was named Tomo_WT_Initiation, the tilt series was recorded at 11500X, resulted in a pixel size of 0.953 nm. The tomogram was binned by 2 for processing so the pixel size is 1.9058 nm.\nImage size 2986 x 3068 pixels, 384 sections\nThe segmentation was named Segmentation_WT_initiation","image_width":null,"image_height":"384"},{"segmentations":[{"file":"data/PbSFA_Tomo/Figure8B/Segmentation_WT_elongation.mod","description":"Both tomogram reconstruction and segmentation were performed with IMOD","original_files":"data/PbSFA_Tomo/Figure8B/Segmentation_WT_elongation.mod","original_format":"MOD","entry":null}],"name":"Electron tomograms of WT budding sporozoites at elongation stage","directory":"data/PbSFA_Tomo/Figure8B","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.9058,"pixel_height":1.9058,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The tomogram used to generate figure8B was named Tomo_WT_elongation, the tilt series was recorded at 11500X, resulted in a pixel size of 0.953 nm. The tomogram was binned by 2 for processing so the pixel size is 1.9058 nm.\nImage size 3076 x 3452, 645 sections\nThe segmentation was named Segmentation_WT_elongation","image_width":null,"image_height":"645"},{"segmentations":[{"file":"data/PbSFA_Tomo/Figure8C/Segmentation_SFA1KO_initiation.mod","description":"Both tomogram reconstruction and segmentation were performed with IMOD","original_files":"data/PbSFA_Tomo/Figure8C/Segmentation_SFA1KO_initiation.mod","original_format":"MOD","entry":null}],"name":"Electron tomograms of SFA1KO budding sporozoites at initiation stage","directory":"data/PbSFA_Tomo/Figure8C","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.9058,"pixel_height":1.9058,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The tomogram used to generate figure8C was named Tomo_SFA1KO_initiation, the tilt series was recorded at 11500X, resulted in a pixel size of 0.953 nm. The tomogram was binned by 2 for processing so the pixel size is 1.9058 nm.\nImage size 2687 x 2330, 357 sections.\nThe segmentation was named Segmentation_SFA1KO_initiation","image_width":null,"image_height":"357"},{"segmentations":[{"file":"data/PbSFA_Tomo/Figure8D/Segmentation_SFA1KO_elongation.mod","description":"Both tomogram reconstruction and segmentation were performed with IMOD","original_files":"data/PbSFA_Tomo/Figure8D/Segmentation_SFA1KO_elongation.mod","original_format":"MOD","entry":null}],"name":"Electron tomograms of SFA1KO budding sporozoites at elongation stage","directory":"data/PbSFA_Tomo/Figure8D","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":2,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.9058,"pixel_height":1.9058,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The tomogram used to generate figure8D was named Tomo_SFA1KO_elongation, the tilt series was recorded at 11500X, resulted in a pixel size of 0.953 nm. The tomogram was binned by 2 for processing so the pixel size is 1.9058 nm.\nImage size 2826 x 2811, 899 sections.\nThe segmentation was named Segmentation_SFA1KO_elongation, and was drawn on the further binned tomogram Tomo_SFA1KO_elongation_bin with pixel size of 3.8116 nm","image_width":null,"image_height":"899"},{"segmentations":[{"file":"data/PbSFA_Tomo/Figure8E/Segmentation_SFA2KO_initiation.mod","description":"Both tomogram reconstruction and segmentation were performed with IMOD","original_files":"data/PbSFA_Tomo/Figure8E/Segmentation_SFA2KO_initiation.mod","original_format":"MOD","entry":null}],"name":"Electron tomograms of SFA2KO budding sporozoites at initiation stage","directory":"data/PbSFA_Tomo/Figure8E","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":2,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.9058,"pixel_height":1.9058,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The tomogram used to generate figure8E was named Tomo_SFA2KO_initiation, the tilt series was recorded at 11500X, resulted in a pixel size of 0.953 nm. The tomogram was binned by 2 for processing so the pixel size is 1.9058 nm.\nImage size 2257 x 2251, 342 sections.\nThe segmentation was named Segmentation_SFA2KO_initiation, and was drawn on a older version of tomogram Tomo_SFA2KO_initiation_old, the quality of which was not ideal. This one was uploaded only to help visualize the segmentation","image_width":null,"image_height":"342"},{"segmentations":[{"file":"data/PbSFA_Tomo/Figure8F/Segmentation_SFA2KO_elongation.mod","description":"Both tomogram reconstruction and segmentation were performed with IMOD","original_files":"data/PbSFA_Tomo/Figure8F/Segmentation_SFA2KO_elongation.mod","original_format":"MOD","entry":null}],"name":"Electron tomograms of SFA2KO budding sporozoites at elongation stage","directory":"data/PbSFA_Tomo/Figure8F","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":2.236,"pixel_height":2.236,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The tomogram used to generate figure8F was named Tomo_SFA2KO_elongation, the tilt series was recorded at 9600X, resulted in a pixel size of 1.12 nm. The tomogram was binned by 2 for processing so the pixel size is 2.236 nm.\nImage size 2674 x 2679, 780 sections.\nThe segmentation was named Segmentation_SFA2KO_elongation","image_width":null,"image_height":"780"}],"workflow_file":null,"grant_references":[{"funding_body":"German Research Foundation (DFG)","code":"FR2140/12-1","country":"Germany"}],"version_history":[],"title":"Electron tomography reveals perturbed inter-organellar relationship in PbSFA mutants","principal_investigator":[{"author_orcid":null,"middle_name":null,"organization":"Division of Infectious Diseases, Boston Children’s Hospital","street":null,"town_or_city":"Boston","state_or_province":"Massachusetts","post_or_zip":"02115","telephone":null,"fax":null,"first_name":"Jeffrey","last_name":"Dvorin","email":"Jeffrey.Dvorin [at] childrens.harvard.edu","country":"United States","entry":"EMPIAR-13387"},{"author_orcid":null,"middle_name":null,"organization":"Department of Parasitology, Center of Infectious Diseases, Medical Faculty, Heidelberg University","street":"Im Neuenheimer Feld 324","town_or_city":"Heidelberg","state_or_province":"Baden Wuertemberg","post_or_zip":"69120","telephone":null,"fax":null,"first_name":"Friedrich","last_name":"Frischknecht","email":"freddy.frischknecht [at] med.uni-heidelberg.de","country":"Germany","entry":"EMPIAR-13387"}],"status":"REL","deposition_date":"2026-03-19","release_date":"2026-06-23","obsolete_date":null,"update_date":"2026-06-23","corresponding_author":{"author":{"author_orcid":"0009-0001-2854-6369","middle_name":null,"organization":"Department of Parasitology, Center of Infectious Diseases, Heidelberg University","street":null,"town_or_city":"Heidelberg","state_or_province":null,"post_or_zip":"69120","first_name":"Buyuan","last_name":"He","country":"Germany"}},"authors":[{"author":{"name":"Ali I","author_orcid":null}},{"author":{"name":"Dorner L","author_orcid":null}},{"author":{"name":"Chowdhury MR","author_orcid":null}},{"author":{"name":"Sokolowski-Adams Y","author_orcid":null}},{"author":{"name":"Abadzhieva E","author_orcid":null}},{"author":{"name":"Reich A","author_orcid":null}},{"author":{"name":"Wittmaack M","author_orcid":null}},{"author":{"name":"Sattler JM","author_orcid":null}},{"author":{"name":"Frischknecht F","author_orcid":null}},{"author":{"name":"Dvorin JD","author_orcid":null}}],"cross_references":[],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"He B","author_orcid":"0009-0001-2854-6369"}],"editors":[],"published":false,"j_or_nj_citation":true,"title":"Essential connection between nucleus and apical pole maintains fidelity during Plasmodium progeny formation across the vertebrate and mosquito hosts","volume":null,"country":"Germany","first_page":null,"last_page":null,"year":null,"language":"English","doi":null,"pubmedid":null,"details":"Plasmodium parasites, the causative agents of malaria, undergo complex replication within both vertebrate and insect hosts, presenting unique opportunities for therapeutic intervention. A key challenge during these replication events, i.e., schizogony in vertebrate red blood cells and sporogony in oocysts within mosquitos, is ensuring the faithful partitioning of nuclei and organelles into the numerous daughter cells that form at once from a single parent. While nuclear microtubule-organizing centers, or centriolar plaques (CPs), have been hypothesized to play a central role in this process, the molecular mediators linking the CPs and organelles remain incompletely defined. Here, we characterize two striated fiber assemblin (SFA) homologs, SFA1 and SFA2, in Plasmodium falciparum and Plasmodium berghei across two hosts. We show that these SFAs form a physical bridge between the CP and the nascent apical poles of daughter cells, facilitating high-fidelity progeny formation during schizogony and sporogony. Loss of SFA function disrupts merozoite and sporozoite formation, with profound consequences for transmission. These findings establish SFAs as essential organizers of parasite morphogenesis and highlight them as potential targets for antimalarial therapies.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"The EMBO Journal","journal_abbreviation":"EMBO J","issue":null,"preprint":false}],"dataset_size":"27.2 GB","experiment_type":"ssET","scale":"cell","entry_doi":"10.6019/EMPIAR-13387"}}