{"EMPIAR-13371":{"imagesets":[{"segmentations":[],"name":"Movies of CbAgo catalytic mutant in ternary form, complexed with guide DNA, target DNA, and de novo designed dimer disrupting binder Dimbd2","directory":"data","category":"micrographs - multiframe","header_format":"EER","data_format":"EER","num_images_or_tilt_series":12538,"frames_per_image":50,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.57,"pixel_height":0.57,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Sample of catalytic double mutant D541A, D611A of Clostridium butyricum Argonaute in ternary form (mix of monomers and dimers), bound to single stranded guide DNA ([P]-ATTTATGTGATTTTTC), single stranded target DNA (GAAAAATCACATAAAT), and BindCraft-designed de novo dimer disrupting binder Dimbd2 (KSFEEKKEIVIGMLRYMLENFEEYVELYPDNEVLQTLVPEFKKLVEELESETDKEKFLEKLKEISEIYAKLLKEIFGDNGRSLEELKEKIFVHFSGALE). Purified in buffer 20 mM Tris-HCl pH 7.5, 250 mM KCl, 5% glycerol.\n50 frame movies, with a 50 e/A^2 electron dose, on a 300 kV Titan Krios with Falcon 4i detector, with 2.7 mm C2 lens, collected with a 0.57 A pixel size.","image_width":"4096","image_height":"4096"}],"workflow_file":null,"grant_references":[{"funding_body":"Swiss National Science Foundation","code":"310030188744","country":"Switzerland"}],"version_history":[],"title":"Clostridium butyricum Argonaute catalytic mutant in ternary form (bound to guide DNA and target DNA) and de novo designed Dimbd2","principal_investigator":[{"author_orcid":"0000-0003-1560-5769","middle_name":null,"organization":"University of Zurich","street":"Winterthurerstrasse 190","town_or_city":"Zurich","state_or_province":null,"post_or_zip":"8051","telephone":null,"fax":null,"first_name":"Martin","last_name":"Pacesa","email":"pacesa [at] pharma.uzh.ch","country":"Switzerland","entry":"EMPIAR-13371"},{"author_orcid":null,"middle_name":null,"organization":"EPFL","street":null,"town_or_city":"Lausanne","state_or_province":null,"post_or_zip":"1015","telephone":null,"fax":null,"first_name":"Bruno","last_name":"Correia","email":"bruno.correia [at] epfl.ch","country":"Switzerland","entry":"EMPIAR-13371"}],"status":"REL","deposition_date":"2026-03-14","release_date":"2026-03-18","obsolete_date":null,"update_date":"2026-03-18","corresponding_author":{"author":{"author_orcid":"0000-0003-1560-5769","middle_name":null,"organization":"University of Zurich","street":"Winterthurerstrasse 190","town_or_city":"Zurich","state_or_province":null,"post_or_zip":"8051","first_name":"Martin","last_name":"Pacesa","country":"Switzerland"}},"authors":[{"author":{"name":"Barendse P","author_orcid":"0000-0002-6332-354X"}},{"author":{"name":"van Drimmelen M","author_orcid":null}},{"author":{"name":"Pacesa M","author_orcid":"0000-0003-1560-5769"}},{"author":{"name":"Sivaraman A","author_orcid":null}},{"author":{"name":"Zhang DK","author_orcid":null}},{"author":{"name":"Nickel L","author_orcid":null}},{"author":{"name":"Niault T","author_orcid":null}},{"author":{"name":"Lindhoud S","author_orcid":null}},{"author":{"name":"Roosjen M","author_orcid":null}},{"author":{"name":"Hegge JW","author_orcid":null}},{"author":{"name":"Park K","author_orcid":null}},{"author":{"name":"Correia BE","author_orcid":null}},{"author":{"name":"van der Oost J","author_orcid":null}},{"author":{"name":"Joo C","author_orcid":null}},{"author":{"name":"Westphal AH","author_orcid":null}},{"author":{"name":"Swarts DC","author_orcid":"0000-0003-4412-9191"}}],"cross_references":[],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Barendse P","author_orcid":"0000-0002-6332-354X"},{"name":"van Drimmelen M","author_orcid":null},{"name":"Pacesa M","author_orcid":"0000-0003-1560-5769"},{"name":"Sivaraman A","author_orcid":null},{"name":"Zhang DK","author_orcid":null},{"name":"Nickel L","author_orcid":null},{"name":"Niault T","author_orcid":null},{"name":"Lindhoud S","author_orcid":null},{"name":"Roosjen M","author_orcid":null},{"name":"Hegge JW","author_orcid":null},{"name":"Park K","author_orcid":null},{"name":"Correia BE","author_orcid":null},{"name":"van der Oost J","author_orcid":null},{"name":"Joo C","author_orcid":null},{"name":"Westphal AH","author_orcid":null},{"name":"Swarts DC","author_orcid":"0000-0003-4412-9191"}],"editors":[],"published":false,"j_or_nj_citation":true,"title":"Target-DNA binding-induced dimerization is required for catalytic activation of Clostridium butyricum Argonaute","volume":null,"country":"","first_page":null,"last_page":null,"year":"2026","language":"English","doi":null,"pubmedid":null,"details":"Sample of catalytic double mutant D541A, D611A of Clostridium butyricum Argonaute in ternary form (mix of monomers and dimers), bound to single stranded guide DNA ([P]-ATTTATGTGATTTTTC), single stranded target DNA (GAAAAATCACATAAAT), and BindCraft-designed de novo dimer disrupting binder Dimbd2 (KSFEEKKEIVIGMLRYMLENFEEYVELYPDNEVLQTLVPEFKKLVEELESETDKEKFLEKLKEISEIYAKLLKEIFGDNGRSLEELKEKIFVHFSGALE). Purified in buffer 20 mM Tris-HCl pH 7.5, 250 mM KCl, 5% glycerol.\n50 frame movies, with a 50 e/A^2 electron dose, on a 300 kV Titan Krios with Falcon 4i detector, with 2.7 mm C2 lens, collected with a 0.57 A pixel size.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"","journal_abbreviation":"","issue":null,"preprint":false}],"dataset_size":"4.0 TB","experiment_type":"IHM","scale":"molecule","entry_doi":"10.6019/EMPIAR-13371"}}