{"EMPIAR-13358":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame movies of Danio rerio tRNA ligase complex (truncated variant)","directory":"data/Movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":9942,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.84,"pixel_height":0.84,"micrographs_file_pattern":"data/Movies/*.tiff","picked_particles_file_pattern":"data/particles/EMD-52744_particlestack.star","picked_particles_directory":"data/particles","details":"Gain-corrected, unaligned multi-frame movies collected with Krios G3i microscope + K3 detector (superresolution mode, binned 2x with EPU) with 20 deg stage tilt. Voltage: 300 kV; spherical aberration: 2.7; dose per frame: 1 e/A^2 (total dose: 41.09 e/A^2 fractionated over 40 frames). Defocus range (nominal): -2.5, -2.0, -1.5, -0.9 µm. Objective aperture retracted, C2 aperture: 50 µm.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Aligned particles of Danio rerio tRNA ligase complex (truncated variant)","directory":"data/particles","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":179455,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.84,"pixel_height":0.84,"micrographs_file_pattern":"data/Movies/*.tiff","picked_particles_file_pattern":"data/particles/EMD-52744_particlestack.star","picked_particles_directory":"data/particles","details":"Aligned particles with corresponding Relion-3 STAR file. Micrographs were initially binned 2x during Motion correction.","image_width":"256","image_height":"256"}],"workflow_file":null,"grant_references":[{"funding_body":"Polish National Science Centre","code":"2019/33/B/NZ1/02839","country":"Poland"},{"funding_body":"Other government","code":"1/SOL/2021/2","country":"Poland"},{"funding_body":"European Research Council (ERC)","code":"101001394","country":"Poland"}],"version_history":[],"title":"Cryo-EM structure of the Danio rerio tRNA ligase complex","principal_investigator":[{"author_orcid":"0000-0001-8632-0977","middle_name":null,"organization":"International Institute of Molecular and Cell Biology in Warsaw","street":"4 Ks. Trojdena Street","town_or_city":"Warsaw","state_or_province":null,"post_or_zip":"02-109","telephone":null,"fax":null,"first_name":"Marcin","last_name":"Nowotny","email":"mnowotny [at] iimcb.gov.pl","country":"Poland","entry":"EMPIAR-13358"}],"status":"REL","deposition_date":"2026-02-16","release_date":"2026-03-16","obsolete_date":null,"update_date":"2026-03-16","corresponding_author":{"author":{"author_orcid":"0000-0001-5582-2851","middle_name":null,"organization":"International Institute of Molecular and Cell Biology in Warsaw","street":"4 Ks. Trojdena Street","town_or_city":"Warsaw","state_or_province":null,"post_or_zip":"02-106","first_name":"Mariusz","last_name":"Czarnocki-Cieciura","country":"Poland"}},"authors":[{"author":{"name":"Chamera S","author_orcid":"0000-0002-2328-9703"}},{"author":{"name":"Zajko W","author_orcid":"0000-0001-9915-0305"}},{"author":{"name":"Czarnocki-Cieciura M","author_orcid":"0000-0001-5582-2851"}},{"author":{"name":"Jaciuk M","author_orcid":"0000-0003-1251-1492"}},{"author":{"name":"Koziej Ł","author_orcid":"0000-0003-3885-3845"}},{"author":{"name":"Nowak J","author_orcid":"0000-0001-6536-2940"}},{"author":{"name":"Wycisk K","author_orcid":"0000-0002-0999-0560"}},{"author":{"name":"Sroka M","author_orcid":"0000-0002-3865-1218"}},{"author":{"name":"Chramiec-Głąbik A","author_orcid":"0000-0002-3544-7211"}},{"author":{"name":"Śmietański M","author_orcid":"0000-0002-9482-6588"}},{"author":{"name":"Gołębiowski F","author_orcid":"0000-0002-8011-2162"}},{"author":{"name":"Warmiński M","author_orcid":"0000-0002-1071-3405"}},{"author":{"name":"Jemielity J","author_orcid":"0000-0001-7633-788X"}},{"author":{"name":"Glatt S","author_orcid":"0000-0003-2815-7133"}},{"author":{"name":"Nowotny M","author_orcid":"0000-0001-8632-0977"}}],"cross_references":["EMD-52744"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Chamera S","author_orcid":"0000-0002-2328-9703"},{"name":"Zajko W","author_orcid":"0000-0001-9915-0305"},{"name":"Czarnocki-Cieciura M","author_orcid":"0000-0001-5582-2851"},{"name":"Jaciuk M","author_orcid":"0000-0003-1251-1492"},{"name":"Koziej Ł","author_orcid":"0000-0003-3885-3845"},{"name":"Nowak J","author_orcid":"0000-0001-6536-2940"},{"name":"Wycisk K","author_orcid":"0000-0002-0999-0560"},{"name":"Sroka M","author_orcid":"0000-0002-3865-1218"},{"name":"Chramiec-Głąbik A","author_orcid":"0000-0002-3544-7211"},{"name":"Śmietański M","author_orcid":"0000-0002-9482-6588"},{"name":"Gołębiowski F","author_orcid":"0000-0002-8011-2162"},{"name":"Warmiński M","author_orcid":"0000-0002-1071-3405"},{"name":"Jemielity J","author_orcid":"0000-0001-7633-788X"},{"name":"Glatt S","author_orcid":"0000-0003-2815-7133"},{"name":"Nowotny M","author_orcid":"0000-0001-8632-0977"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structural and biochemical characterization of the 3'-5' tRNA splicing ligases","volume":"301","country":"","first_page":null,"last_page":null,"year":"2025","language":"English","doi":"10.1016/j.jbc.2025.108506","pubmedid":"40220997","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"The Journal of biological chemistry","journal_abbreviation":"J Biol Chem","issue":"5","preprint":false}],"dataset_size":"3.7 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9i8v"],"entry_doi":"10.6019/EMPIAR-13358"}}