{"EMPIAR-13331":{"imagesets":[{"segmentations":[],"name":"Raw cryoET data from collection on 7/18/2025","directory":"data/Dataset1_frames","category":"micrographs - multiframe","header_format":"EER","data_format":"EER","num_images_or_tilt_series":4012,"frames_per_image":224,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":1.561,"pixel_height":1.561,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"There are 224 EER frames per image, and each tilt series is comprised of up to 37 tilts. Some of them have fewer tilts because the stage has a maximum tilt angle and some of the targets had a larger estimated pre-tilt while setting up paceTOMO. Associated metadata from SerialEM is in the mdocs folder.","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"Raw cryoET data from collection on 7/19/2025","directory":"data/Dataset2_frames","category":"micrographs - multiframe","header_format":"EER","data_format":"EER","num_images_or_tilt_series":5884,"frames_per_image":224,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":1.561,"pixel_height":1.561,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"There are 224 EER frames per image, and each tilt series is comprised of up to 37 tilts. Some of them have fewer tilts because the stage has a maximum tilt angle and some of the targets had a larger estimated pre-tilt while setting up paceTOMO. Associated metadata from SerialEM is in the mdocs folder.","image_width":"4096","image_height":"4096"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)","code":"U54 AI170856","country":"United States"}],"version_history":[],"title":"Cryo electron tomography of BG505 HIV-1 Env in a virus-like particle","principal_investigator":[{"author_orcid":"0000-0003-4677-9809","middle_name":null,"organization":"Department of Molecular Biology, University of California San Diego, La Jolla, CA, USA","street":"9500 Gilman Drive","town_or_city":"La Jolla","state_or_province":"CA","post_or_zip":"92093","telephone":null,"fax":null,"first_name":"Elizabeth","last_name":"Villa","email":"evilla [at] ucsd.edu","country":"United States","entry":"EMPIAR-13331"}],"status":"REL","deposition_date":"2025-11-06","release_date":"2026-04-09","obsolete_date":null,"update_date":"2026-04-09","corresponding_author":{"author":{"author_orcid":"0009-0001-4737-542X","middle_name":"Camille","organization":"UC San Diego","street":"9500 Gilman Drive","town_or_city":"La Jolla","state_or_province":"CA","post_or_zip":"92093","first_name":"Madeleine","last_name":"Duquette","country":"United States"}},"authors":[{"author":{"name":"Duquette MC","author_orcid":"0009-0001-4737-542X"}}],"cross_references":["EMD-75641"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Shehata M","author_orcid":"0000-0003-0841-4577"},{"name":"Casalino L","author_orcid":"0000-0003-3581-1148"},{"name":"Duquette M","author_orcid":"0009-0001-4737-542X"},{"name":"Chen S","author_orcid":"0000-0003-4565-4772"},{"name":"Flaherty A","author_orcid":"0009-0001-2779-2082"},{"name":"Villa E","author_orcid":"0000-0003-4677-9809"},{"name":"Amaro RE","author_orcid":"0000-0002-9275-9553"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"N-Glycans Modulate HIV-1 Env Conformational Plasticity","volume":null,"country":"United States","first_page":null,"last_page":null,"year":"2025","language":"English","doi":"10.1101/2025.03.26.645577","pubmedid":null,"details":"The micrographs were collected on a Titan Krios G4 TEM (Thermo Fisher) operating at 300 Kv with a Falcon 4i direct electron detector (Thermo Fisher) at a nominal magnification of 81,000x (pixel size 1.561 Å, counting mode). A Selectris X energy filter with a 10 eV-wide slit was used throughout data collection. PaceTOMO was used to collect tilt series. 224 EER frames were collected per tilt series at a dose rate of 4.4 e-/Å2/s.  The raw EER frames are provided, as well as the mdocs.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"bioRxiv","journal_abbreviation":"","issue":null,"preprint":true}],"dataset_size":"963.0 GB","experiment_type":"EMDB","scale":"virus","entry_doi":"10.6019/EMPIAR-13331"}}