{"EMPIAR-13326":{"imagesets":[{"segmentations":[],"name":"Raw unaligned multi-frame micrographs for each tilt in 17 tilt-series in iMC control condition","directory":"data/iMC_control/frames","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":1012,"frames_per_image":10,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":3.37,"pixel_height":3.37,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Raw unaligned multi-frame micrographs were aligned, sorted and combined into tilt series stacks with a serialEM plugin or in Warp (.mrc or .st files, respectively) located in the processing folders.\n\nNaming for tomograms of iMC control cells: TS_01_iMC_control, TS_02_iMC_control, TS_03_iMC_control, TS_04_iMC_control, TS_05_iMC_control, TS_06_iMC_control, TS_07_iMC_control, TS_08_iMC_control, TS_15_iMC_control, TS_16_iMC_control, TS_17_iMC_control, TS_18_iMC_control, TS_19_iMC_control, TS_20_iMC_control, TS_21_iMC_control, TS_23_iMC_control, TS_24_iMC_control. \n\nMetadata files (.mdoc) are provided in the metadata folders. The gain references (.dm4)  are provided in the frames folders.","image_width":null,"image_height":null},{"segmentations":[],"name":"Reconstructed cryo-electron tomograms acquired on cryo-FIB lamellae between two adjacent NIH 3T3 fibroblasts in iMC control conditions","directory":"data/iMC_control/tomograms","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":17,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":13.48,"pixel_height":13.48,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Tomograms (4-times binned reconstructions, original unbinned pixel size 3.37 Å) from aligned frame stacks (.mrc or .st files located in processing folders, .tif files in frames folders).\nMetadata files (.mdoc) are provided in the metadata folders and alignment files (.xf) are in the processing folders.\n\nNaming for tomograms of iMC control cells: TS_01_iMC_control, TS_02_iMC_control, TS_03_iMC_control, TS_04_iMC_control, TS_05_iMC_control, TS_06_iMC_control, TS_07_iMC_control, TS_08_iMC_control, TS_15_iMC_control, TS_16_iMC_control, TS_17_iMC_control, TS_18_iMC_control, TS_19_iMC_control, TS_20_iMC_control, TS_21_iMC_control, TS_23_iMC_control, TS_24_iMC_control. \nFor each tomogram, actin and plasma membrane segmentations are provided in their corresponding labels folders.","image_width":null,"image_height":null},{"segmentations":[],"name":"Raw unaligned multi-frame micrographs for each tilt in 9 tilt-series in iMC induced condition","directory":"data/iMC_induced/frames","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":524,"frames_per_image":10,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":3.37,"pixel_height":3.37,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Raw unaligned multi-frame micrographs were aligned, sorted and combined into tilt series stacks in Warp (.st files) located in the processing folders.\nNaming for tomograms of iMC induced cells: TS_09_iMC_induced, TS_10_iMC_induced, TS_11_iMC_induced, TS_12_iMC_induced, TS_13_iMC_induced, TS_14_iMC_induced, TS_25_iMC_induced, TS_26_iMC_induced, TS_28_iMC_induced.\nMetadata files (.mdoc) are provided in the metadata folders. The gain references (.dm4) are provided in the frames folders.","image_width":null,"image_height":null},{"segmentations":[],"name":"Reconstructed cryo-electron tomograms acquired on cryo-FIB lamellae between two adjacent NIH 3T3 fibroblasts in iMC induced conditions","directory":"data/iMC_induced/tomograms","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":9,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":13.48,"pixel_height":13.48,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Tomograms (4-times binned reconstructions, original unbinned pixel size 3.37 Å) from aligned frame stacks (.st files located in processing folders, .tif files in frames folders).\nMetadata files (.mdoc) are provided in the metadata folders and alignment files (.xf) are in the processing folders.\nNaming for tomograms of iMC induced cells: TS_09_iMC_induced, TS_10_iMC_induced, TS_11_iMC_induced, TS_12_iMC_induced, TS_13_iMC_induced, TS_14_iMC_induced, TS_25_iMC_induced, TS_26_iMC_induced, TS_28_iMC_induced.\nFor each tomogram, actin and plasma membrane segmentations are provided in their corresponding labels folders.","image_width":null,"image_height":null},{"segmentations":[],"name":"Raw unaligned multi-frame micrographs for each tilt in 6 tilt-series in CAEzrin control condition","directory":"data/CAEzrin_control/frames","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":349,"frames_per_image":10,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":3.37,"pixel_height":3.37,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Raw unaligned multi-frame micrographs were aligned, sorted and combined into tilt series stacks in Warp (.st files) located in the processing folders.\nNaming for tomograms of CAEzrin control cells: TS_43_CAEz_control, TS_45_CAEz_control, TS_47_CAEz_control, TS_48_CAEz_control, TS_49_CAEz_control, TS_50_CAEz_control.\nMetadata files (.mdoc) are provided in the metadata folders. The gain references (.dm4) are provided in the frames folders.","image_width":null,"image_height":null},{"segmentations":[],"name":"Reconstructed cryo-electron tomograms acquired on cryo-FIB lamellae between two adjacent NIH 3T3 fibroblasts in CAEzrin control conditions","directory":"data/CAEzrin_control/tomograms","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":6,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":13.48,"pixel_height":13.48,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Tomograms (4-times binned reconstructions, original unbinned pixel size 3.37 Å) from aligned frame stacks (.st files located in processing folders, .tif files in frames folders).\nMetadata files (.mdoc) are provided in the metadata folders and alignment files (.xf) are in the processing folders.\nNaming for tomograms of CAEzrin control cells: TS_43_CAEz_control, TS_45_CAEz_control, TS_47_CAEz_control, TS_48_CAEz_control, TS_49_CAEz_control, TS_50_CAEz_control.\nFor each tomogram, actin and plasma membrane segmentations are provided in their corresponding labels folders.","image_width":null,"image_height":null},{"segmentations":[],"name":"Raw unaligned multi-frame micrographs for each tilt in 20 tilt-series in CAEzrin induced condition","directory":"data/CAEzrin_control/frames","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":1041,"frames_per_image":10,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":3.37,"pixel_height":3.37,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Raw unaligned multi-frame micrographs were aligned, sorted and combined into tilt series stacks with a serialEM plugin or in Warp (.mrc or .st files, respectively) located in the processing folders.\nNaming for tomograms of CAEzrin induced cells: TS_35_CAEz_induced, TS_36_CAEz_induced, TS_37_CAEz_induced, TS_38_CAEz_induced, TS_39_CAEz_induced, TS_40_CAEz_induced, TS_41_CAEz_induced, TS_42_CAEz_induced, TS_51_CAEz_induced, TS_52_CAEz_induced, TS_53_CAEz_induced, TS_54_CAEz_induced, TS_55_CAEz_induced, TS_56_CAEz_induced, TS_57_CAEz_induced, TS_58_CAEz_induced, TS_59_CAEz_induced, TS_60_CAEz_induced, TS_61_CAEz_induced, TS_62_CAEz_induced.\nMetadata files (.mdoc) are provided in the metadata folders. The gain references (.dm4) are provided in the frames folders.","image_width":null,"image_height":null},{"segmentations":[],"name":"Reconstructed cryo-electron tomograms acquired on cryo-FIB lamellae between two adjacent NIH 3T3 fibroblasts in CAEzrin induced conditions","directory":"data/CAEzrin_induced/tomograms","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":20,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":13.48,"pixel_height":13.48,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Tomograms (4-times binned reconstructions, original unbinned pixel size 3.37 Å) from aligned frame stacks (.st files located in processing folders, .tif files in frames folders).\nMetadata files (.mdoc) are provided in the metadata folders and alignment files (.xf) are in the processing folders.\nNaming for tomograms of CAEzrin induced cells: TS_35_CAEz_induced, TS_36_CAEz_induced, TS_37_CAEz_induced, TS_38_CAEz_induced, TS_39_CAEz_induced, TS_40_CAEz_induced, TS_41_CAEz_induced, TS_42_CAEz_induced, TS_51_CAEz_induced, TS_52_CAEz_induced, TS_53_CAEz_induced, TS_54_CAEz_induced, TS_55_CAEz_induced, TS_56_CAEz_induced, TS_57_CAEz_induced, TS_58_CAEz_induced, TS_59_CAEz_induced, TS_60_CAEz_induced, TS_61_CAEz_induced, TS_62_CAEz_induced.\nFor each tomogram, actin and plasma membrane segmentations are provided in their corresponding labels folders.","image_width":null,"image_height":null},{"segmentations":[],"name":"Raw unaligned multi-frame micrographs for each tilt in 8 tilt-series in 6FP-iMC control condition","directory":"data/6FP_control/frames","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":406,"frames_per_image":10,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":3.37,"pixel_height":3.37,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Raw unaligned multi-frame micrographs were aligned, sorted and combined into tilt series stacks in Warp (.st files) located in the processing folders.\nNaming for tomograms of 6FP-iMC control cells: TS_90_6FP_control, TS_91_6FP_control, TS_94_6FP_control, TS_95_6FP_control, TS_96_6FP_control, TS_103_6FP_control, TS_104_6FP_control, TS_105_6FP_control.\nMetadata files (.mdoc) are provided in the metadata folders. The gain references (.dm4) are provided in the frames folders.","image_width":null,"image_height":null},{"segmentations":[],"name":"Reconstructed cryo-electron tomograms acquired on cryo-FIB lamellae between two adjacent NIH 3T3 fibroblasts in 6FP-iMC control conditions","directory":"data/6FP_control/tomograms","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":8,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":13.48,"pixel_height":13.48,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Tomograms (4-times binned reconstructions, original unbinned pixel size 3.37 Å) from aligned frame stacks (.st files located in processing folders, .tif files in frames folders).\nMetadata files (.mdoc) are provided in the metadata folders and alignment files (.xf) are in the processing folders.\nNaming for tomograms of 6FP-iMC control cells: TS_90_6FP_control, TS_91_6FP_control, TS_94_6FP_control, TS_95_6FP_control, TS_96_6FP_control, TS_103_6FP_control, TS_104_6FP_control, TS_105_6FP_control.\nFor each tomogram, actin and plasma membrane segmentations are provided in their corresponding labels folders.","image_width":null,"image_height":null},{"segmentations":[],"name":"Raw unaligned multi-frame micrographs for each tilt in 10 tilt-series in 6FP-iMC induced condition","directory":"data/6FP_induced/frames","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":539,"frames_per_image":10,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":3.37,"pixel_height":3.37,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Raw unaligned multi-frame micrographs were aligned, sorted and combined into tilt series stacks in Warp (.st files) located in the processing folders.\nNaming for tomograms of 6FP-iMC induced cells: TS_106_6FP_induced, TS_107_6FP_induced, TS_109_6FP_induced, TS_110_6FP_induced, TS_111_6FP_induced, TS_113_6FP_induced, TS_114_6FP_induced, TS_116_6FP_induced, TS_117_6FP_induced, TS_118_6FP_induced.\nMetadata files (.mdoc) are provided in the metadata folders. The gain references (.dm4) are provided in the frames folders.","image_width":null,"image_height":null},{"segmentations":[],"name":"Reconstructed cryo-electron tomograms acquired on cryo-FIB lamellae between two adjacent NIH 3T3 fibroblasts in 6FP-iMC induced conditions","directory":"data/6FP_induced/tomograms","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":10,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":13.48,"pixel_height":13.48,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Tomograms (4-times binned reconstructions, original unbinned pixel size 3.37 Å) from aligned frame stacks (.st files located in processing folders, .tif files in frames folders).\nMetadata files (.mdoc) are provided in the metadata folders and alignment files (.xf) are in the processing folders.\nNaming for tomograms of 6FP-iMC induced cells: TS_106_6FP_induced, TS_107_6FP_induced, TS_109_6FP_induced, TS_110_6FP_induced, TS_111_6FP_induced, TS_113_6FP_induced, TS_114_6FP_induced, TS_116_6FP_induced, TS_117_6FP_induced, TS_118_6FP_induced.\nFor each tomogram, actin and plasma membrane segmentations are provided in their corresponding labels folders.","image_width":null,"image_height":null}],"workflow_file":null,"grant_references":[{"funding_body":"European Research Council (ERC)","code":"101124221","country":"Germany"},{"funding_body":"German Research Foundation (DFG)","code":"DI 2205/3-1","country":"Germany"},{"funding_body":"Human Frontier Science Program (HFSP)","code":"RGY0073/2018","country":"Germany"},{"funding_body":"EIPOD fellowship under Marie Sklodowska-Curie Actions COFUND","code":"847532","country":"Germany"},{"funding_body":"EIPOD fellowship under Marie Sklodowska-Curie Actions COFUND","code":"664726","country":"Germany"},{"funding_body":"European Molecular Biology Organization (EMBO)","code":"ALTF-2024","country":"Germany"},{"funding_body":"Boehringer Ingelheim Fonds (BIF)","code":"-","country":"Germany"}],"version_history":[],"title":"Cryo-electron tomography of the actin cortex of NIH 3T3 fibroblasts upon regulation of membrane-to-cortex attachment","principal_investigator":[{"author_orcid":"0000-0001-6968-041X","middle_name":null,"organization":"EMBL","street":null,"town_or_city":"Heidelberg","state_or_province":null,"post_or_zip":"69117","telephone":null,"fax":null,"first_name":"Julia","last_name":"Mahamid","email":"julia.mahamid [at] embl.de","country":"Germany","entry":"EMPIAR-13326"},{"author_orcid":"0000-0001-6864-8901","middle_name":null,"organization":"EMBL","street":null,"town_or_city":"Heidelberg","state_or_province":null,"post_or_zip":"69117","telephone":null,"fax":null,"first_name":"Alba","last_name":"Diz-Munoz","email":"alba.dizmunoz [at] embl.de","country":"Germany","entry":"EMPIAR-13326"}],"status":"REL","deposition_date":"2025-12-08","release_date":"2026-05-13","obsolete_date":null,"update_date":"2026-05-13","corresponding_author":{"author":{"author_orcid":"0000-0002-2842-0360","middle_name":"François","organization":"EMBL","street":null,"town_or_city":"Heidelberg","state_or_province":null,"post_or_zip":"69117","first_name":"Samuel","last_name":"Gerard","country":"Germany"}},"authors":[{"author":{"name":"Gérard SF","author_orcid":"0000-0002-2842-0360"}},{"author":{"name":"Cheng DCW","author_orcid":"0000-0003-1545-0269"}},{"author":{"name":"Toro-Nahuelpan M","author_orcid":"0000-0001-5333-3640"}},{"author":{"name":"Mahamid J","author_orcid":"0000-0001-6968-041X"}},{"author":{"name":"Diz-Munoz A","author_orcid":"0000-0001-6864-8901"}}],"cross_references":[],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Strauss L","author_orcid":"0000-0002-0680-593X"},{"name":"Lembo S","author_orcid":"0000-0002-2253-8771"},{"name":"Gérard SF","author_orcid":"0000-0002-2842-0360"},{"name":"Siggel M","author_orcid":"0000-0003-3393-4988"},{"name":"Cheng DCW","author_orcid":"0000-0003-1545-0269"},{"name":"Bergert M","author_orcid":"0000-0002-3222-8929"},{"name":"Foster SK","author_orcid":"0009-0002-4746-3339"},{"name":"Vermeil J","author_orcid":"0000-0001-9636-739X"},{"name":"Toro-Nahuelpan M","author_orcid":"0000-0001-5333-3640"},{"name":"Fischer L","author_orcid":"0000-0002-6885-1210"},{"name":"Yu Q","author_orcid":"0000-0002-4652-0795"},{"name":"Sitarska E","author_orcid":"0000-0001-6910-0876"},{"name":"Chan CJ","author_orcid":"0000-0003-3177-3949"},{"name":"Kosinski J","author_orcid":"0000-0002-3641-0322"},{"name":"Piel M","author_orcid":null},{"name":"Du Roure O","author_orcid":"0000-0002-6364-612X"},{"name":"Heuvingh J","author_orcid":"0000-0002-6851-584X"},{"name":"Mahamid J","author_orcid":"0000-0001-6968-041X"},{"name":"Diz-Munoz A","author_orcid":"0000-0001-6864-8901"}],"editors":[],"published":false,"j_or_nj_citation":true,"title":"The membrane-to-cortex distance gates mDia1 activity to control cortical mechanics","volume":null,"country":"","first_page":null,"last_page":null,"year":null,"language":null,"doi":"10.1038/s41467-026-72845-3","pubmedid":null,"details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"","journal_abbreviation":"","issue":null,"preprint":false}],"dataset_size":"804.3 GB","experiment_type":"EMDB","scale":"cell","entry_doi":"10.6019/EMPIAR-13326"}}