{"EMPIAR-13314":{"imagesets":[{"segmentations":[],"name":"raw eer of tilt series","directory":"data/eer","category":"tilt series","header_format":"EER","data_format":"EER","num_images_or_tilt_series":251,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":1.98,"pixel_height":1.98,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"251 tilt series comprised of 41 tilts with 3 degree spacing between tilts in eer format. Total dose 130e-/A^2 / tilt series for 3.17e-/A^2 / tilt. Data acquired at 64000x with 1.98A / pixel.","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"raw .mrc of tilt series","directory":"data/tilt_mrc","category":"tilt series","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":251,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.98,"pixel_height":1.98,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"251 tilt series comprised of 41 tilts with 3 degree spacing between tilts in mrc format. Total dose 130e-/A^2 / tilt series for 3.17e-/A^2 / tilt. Data acquired at 64000x with 1.98A / pixel.","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"isonet deconvolved bin 8 reconstructions","directory":"data/tomos_isonetdeconv_bin8","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":251,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":15.84,"pixel_height":15.84,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"unfiltered reconstructed tomograms deconvolved (but not missing wedge corrected) in isonet, bin8.","image_width":"512","image_height":"512"}],"workflow_file":null,"grant_references":[{"funding_body":"Wellcome Trust","code":"225902/Z/22/Z","country":"United Kingdom"},{"funding_body":"Wellcome Trust","code":"220526/Z/20/Z","country":"United Kingdom"},{"funding_body":"Wellcome Trust","code":"226810/Z/22/Z","country":"United Kingdom"}],"version_history":[],"title":"cryo electron tomography of a planar lift-out from mouse hippocampus CA1 spanning CA1-py through CA1-slm.","principal_investigator":[{"author_orcid":"0000-0002-2197-2357","middle_name":null,"organization":"Rosalind Franklin Institute","street":"Building R113, Rutherford Appleton Laboratory, Harwell Campus","town_or_city":"Didcot","state_or_province":"oxfordshire","post_or_zip":"OX11 0QX","telephone":null,"fax":null,"first_name":"Calina","last_name":"Glynn","email":"callie.glynn [at] rfi.ac.uk","country":"United Kingdom","entry":"EMPIAR-13314"}],"status":"REL","deposition_date":"2026-02-14","release_date":"2026-03-12","obsolete_date":null,"update_date":"2026-03-12","corresponding_author":{"author":{"author_orcid":"0000-0002-2197-2357","middle_name":null,"organization":"Rosalind Franklin Institute","street":"Building R113, Rutherford Appleton Laboratory, Harwell Campus","town_or_city":"Didcot","state_or_province":"Oxfordshire","post_or_zip":"ox11 0qx","first_name":"Calina","last_name":"Glynn","country":"United Kingdom"}},"authors":[{"author":{"name":"Glynn CG","author_orcid":"0000-0002-2197-2357"}},{"author":{"name":"Smith JLRS","author_orcid":"0000-0003-3234-6228"}}],"cross_references":["EMD-55442"],"biostudies_references":[],"idr_references":[],"empiar_references":[{"name":"EMPIAR-13281"},{"name":"EMPIAR-13288"},{"name":"EMPIAR-13289"},{"name":"EMPIAR-13301"},{"name":"EMPIAR-13302"},{"name":"EMPIAR-13303"},{"name":"EMPIAR-13304"},{"name":"EMPIAR-13287"}],"citation":[{"authors":[{"name":"Glynn C","author_orcid":"0000-0002-2197-2357"},{"name":"Smith JLR","author_orcid":"0000-0003-3234-6228"},{"name":"Case M","author_orcid":"0000-0002-4635-6454"},{"name":"Csöndör R","author_orcid":"0000-0003-1015-8656"},{"name":"Katsini A","author_orcid":"0009-0008-2659-9230"},{"name":"Sanita ME","author_orcid":"0009-0002-0520-5606"},{"name":"Glen TS","author_orcid":"0000-0001-9357-1256"},{"name":"Pennington A","author_orcid":"0000-0002-1179-4243"},{"name":"Grange M","author_orcid":"0000-0003-2580-2299"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"A generalizable and targeted molecular biopsy approach for in situ cryogenic electron tomography of vitreous brain tissue","volume":"5","country":"United States","first_page":null,"last_page":null,"year":"2025","language":"English","doi":"10.1016/j.crmeth.2025.101080","pubmedid":"40527314","details":"Raw eer, mrc, and mdocs and reconstructions of 252 tilt series with 41 total tilts and 130 e-/A2 per position. Planar lift-out spanning mouse hippocampal CA1-py through CA1-slm. Left hemisphere of a 184 day old male mouse. 100 micron thick slice incubation for 15 minutes incubation with cryo protectant 10% dextran 10% sucrose dissolved in NMDG buffer pH 7.4. Tilt series collected at 64000x. Mixed thickness and quality. Whopper015, 065, 079, 105, 171, 188, 229 for process cross sections.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Cell reports methods","journal_abbreviation":"Cell Rep Methods","issue":"7","preprint":false}],"dataset_size":"1.1 TB","experiment_type":"EMDB","scale":"tissue","entry_doi":"10.6019/EMPIAR-13314"}}