{"EMPIAR-13232":{"imagesets":[{"segmentations":[],"name":"Unaligned Movies of E. coli grown at 37°C clarified cell lysate","directory":"data","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":9748,"frames_per_image":45,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":1.06,"pixel_height":1.06,"micrographs_file_pattern":"data/*_fractions.tiff","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Nominal Defocus range (min, max, step): -0.03,-0.2 to -2 in steps of 0.2\nTotal exposure on specimen: 45.1838 el/A2\nExposure time: 3.15 s\nNominal pixel size: 1.06 A\nObjective lens spherical aberration (Cs): 2.7 mm\nAcceleration voltage: 300 kV\nExtraction voltage: 3900 V\nMicroscope / Detector: Krios G3i / K3","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)","code":"R01GM144542","country":"United States"},{"funding_body":"National Science Foundation (NSF, United States)","code":"CAREER-2046778","country":"United States"}],"version_history":[],"title":"Unaligned Movies of E. coli grown at 37°C clarified cell lysate","principal_investigator":[{"author_orcid":"0000-0002-8858-8907","middle_name":null,"organization":"Department of Biology, MIT","street":"31 Ames St","town_or_city":"Cambridge","state_or_province":"MA","post_or_zip":"02139","telephone":null,"fax":null,"first_name":"Joseph","last_name":"Davis","email":"jhdavis [at] mit.edu","country":"United States","entry":"EMPIAR-13232"}],"status":"REL","deposition_date":"2025-12-31","release_date":"2026-02-13","obsolete_date":null,"update_date":"2026-02-13","corresponding_author":{"author":{"author_orcid":"0000-0002-4545-4001","middle_name":null,"organization":"Department of Biology, MIT","street":"31 Ames St","town_or_city":"Cambridge","state_or_province":"MA","post_or_zip":"02139","first_name":"Mira","last_name":"May","country":"United States"}},"authors":[{"author":{"name":"May MB","author_orcid":"0000-0002-4545-4001"}}],"cross_references":["EMD-72030"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"May MB","author_orcid":"0000-0002-4545-4001"},{"name":"Gabriella GS","author_orcid":"0000-0003-1887-477X"},{"name":"Davis JH","author_orcid":"0000-0002-8858-8907"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Capturing ribosomal structures in cellular extracts with cryoPRISM: A purification-free cryoEM approach reveals novel structural states","volume":null,"country":"United States","first_page":null,"last_page":null,"year":null,"language":null,"doi":"10.1101/2025.08.21.669550","pubmedid":"40894545","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Proceedings of the National Academy of Sciences (PNAS)","journal_abbreviation":"","issue":null,"preprint":true}],"dataset_size":"2.4 TB","experiment_type":"EMDB","scale":"cell","related_pdb_entries":["9pyc"],"entry_doi":"10.6019/EMPIAR-13232"}}