{"EMPIAR-13144":{"imagesets":[{"segmentations":[],"name":"Data for the Krios collection where final maps were obtained","directory":"data/Krios_data","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":56478,"frames_per_image":33,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.504,"pixel_height":0.504,"micrographs_file_pattern":"data/Krios_data/raw_movies/GridSquare*/Data/Foil*.tiff","picked_particles_file_pattern":"data/Krios_data/particles_and_related_maps/consensus_all_ribosomes.star","picked_particles_directory":"data/Krios_data/particles_and_related_maps/particle_stacks","details":"Collected on a Titan Krios operated at 300 kV and equipped with a K3 BioQuantum direct electron detector and energy filter using 20 eV slit. The data were acquired at ×165,000 nominal magnification with a calibrated pixel size of 0.504 Å. A total of 56478 were collected in 33 frames with a total dose of 30 e−/Å2 (17 e−/pixel/s) over 0.45 s with a set defocus between−0.7 and−1.0 µm.\nThe movies are not gain corrected. The gain file is provided (data/Krios_data/raw_movies/gain.mrc).\nAll the 70S particles (reference based motion corrected) are provided, as well as star files and maps for the relevant reconstructions (located under \"data/Krios_data/particles_and_related_maps/\" and subfolders, check README).","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Data for the screening done in a Glacios microscope","directory":"data/Glacios_screening_data/raw_movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":39281,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.688,"pixel_height":0.688,"micrographs_file_pattern":"data/Glacios_screening_data/raw_movies/GridSquare*/Data/Foil*.tiff","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Collected on a Glacios operated at 200 kV and equipped with a Falcon 4i direct electron detector and Selectris energy filter using 10 eV slit. The data were acquired at ×165,000 nominal magnification with a calibrated pixel size of 0.504 Å. A total of 39281 were collected in 40 frames with a total dose of 33.18 e−/Å2 (6.67 e−/pixel/s) over 0.45 s with a set defocus between−0.7 and−1.0 µm.\nThe movies are not gain corrected. The gain file is provided (data/Glacios_screening_data/20250507_124636_EER_GainReference.gain).","image_width":"4096","image_height":"4096"}],"workflow_file":null,"grant_references":[{"funding_body":"Swedish Research Council","code":"2022-04511","country":"Sweden"}],"version_history":[],"title":"Single-particle cryo-EM of fusidic acid inhibition of ribosome recycling in Staphylococcus aureus","principal_investigator":[{"author_orcid":"0000-0001-9079-2774","middle_name":null,"organization":"Department of Cell and Molecular Biology (ICM), Uppsala University","street":"Box 596","town_or_city":"Uppsala","state_or_province":"Uppland","post_or_zip":"75124","telephone":null,"fax":null,"first_name":"Maria","last_name":"Selmer","email":"maria.selmer [at] icm.uu.se","country":"Sweden","entry":"EMPIAR-13144"}],"status":"REL","deposition_date":"2025-11-20","release_date":"2026-08-17","obsolete_date":null,"update_date":"2026-08-17","corresponding_author":{"author":{"author_orcid":"0000-0002-4302-6855","middle_name":null,"organization":"Department of Cell and Molecular Biology (ICM), Uppsala University","street":"Box 596","town_or_city":"Uppsala","state_or_province":"Uppland","post_or_zip":"75124","first_name":"Adrián","last_name":"González-López","country":"Sweden"}},"authors":[{"author":{"name":"Gonzalez-Lopez A","author_orcid":"0000-0002-4302-6855"}},{"author":{"name":"Larsson DSD","author_orcid":"0000-0001-7683-6419"}},{"author":{"name":"Selmer M","author_orcid":"0000-0001-9079-2774"}}],"cross_references":["EMD-55945","EMD-55946","EMD-55948","EMD-55944","EMD-55947"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Gonzalez-Lopez A","author_orcid":"0000-0002-4302-6855"},{"name":"Larsson DSD","author_orcid":"0000-0001-7683-6419"},{"name":"Selmer M","author_orcid":"0000-0001-9079-2774"}],"editors":[],"published":false,"j_or_nj_citation":true,"title":"Structural mechanism of ribosome recycling inhibited by fusidic acid","volume":null,"country":"","first_page":null,"last_page":null,"year":null,"language":null,"doi":"10.1093/nar/gkag778","pubmedid":null,"details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"","journal_abbreviation":"","issue":null,"preprint":false}],"dataset_size":"8.8 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9ti6","9ti7","9ti9","9ti5","9ti8"],"entry_doi":"10.6019/EMPIAR-13144"}}