{"EMPIAR-13132":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of Bos taurus mitochondrial complex I from an as-prepared sample solubilised in DDM and reconstituted in to MSP2N2 nanodiscs.","directory":"data","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":6780,"frames_per_image":40,"frame_range_min":1,"frame_range_max":40,"voxel_type":"UNSIGNED BYTE","pixel_width":0.535,"pixel_height":0.535,"micrographs_file_pattern":"data/Dataset*/Raw_Data/*/*/*fractions.tiff","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned multi-frame micrographs of Bos taurus mitochondrial complex I from an as-prepared sample solubilised in DDM and reconstituted in to MSP2N2 nanodiscs. Frozen on 0.6/1 UltrAuFoil grids (though holes were larger). The grid was treated with a PEG-thiol reagent (11-mercaptoundecyl hexaethyleneglycol) beforehand to increase hydrophilicity. Images (TIFF) were collected with a 300 keV FEI Titan Krios on a Falcon K3 detector, using counting mode with super-resolution mode and a Gatan energy filter (20 eV). The C2 and objective lens apertures were set to 70 and 100 µm, respectively. A defocus range of -1.0 to -2.4 was used. 2.4 s exposures were captured at a dose rate of 16.9 electrons Å−2 s−1 in 40 frames with a total dose of 40.5 electrons Å−2. A pixel size of 0.535 Å/pixel (super resolution; nominal pixel size was 1.07 Å/pixel) was used. Datasets with and without AFIS were collected. XML metadata are also included, as are gain reference files and pixel defect files.","image_width":"11520","image_height":"8184"}],"workflow_file":null,"grant_references":[{"funding_body":"Medical Research Council (MRC, United Kingdom)","code":"MC_UU_00015/2","country":"United Kingdom"},{"funding_body":"Sao Paulo Research Foundation (FAPESP)","code":"2019/21856-7","country":"Brazil"},{"funding_body":"Sao Paulo Research Foundation (FAPESP)","code":"2020/14542-3","country":"Brazil"},{"funding_body":"Swiss National Science Foundation","code":"P400PB_191096","country":"Switzerland"}],"version_history":[],"title":"Single particle cryo-EM dataset of as-prepared Bos taurus mitochondrial complex I (oxidised) in MSP2N2","principal_investigator":[{"author_orcid":"0000-0001-8667-6797","middle_name":null,"organization":"MRC MBU, University of Cambridge","street":"Hills Road","town_or_city":"Cambridge","state_or_province":null,"post_or_zip":"CB2 0XY","telephone":null,"fax":null,"first_name":"Judy","last_name":"Hirst","email":"jh480 [at] cam.ac.uk","country":"United Kingdom","entry":"EMPIAR-13132"}],"status":"REL","deposition_date":"2025-11-20","release_date":"2026-03-20","obsolete_date":null,"update_date":"2026-03-20","corresponding_author":{"author":{"author_orcid":"0000-0001-8667-6797","middle_name":null,"organization":"MRC MBU, University of Cambridge","street":"Hills Road","town_or_city":"Cambridge","state_or_province":"Cambridgeshire","post_or_zip":"CB2 0XY","first_name":"Judy","last_name":"Hirst","country":"United Kingdom"}},"authors":[{"author":{"name":"Chung I","author_orcid":"0000-0002-2902-4677"}},{"author":{"name":"Hirst J","author_orcid":"0000-0001-8667-6797"}}],"cross_references":["EMD-14140","EMD-14133","EMD-14132","EMD-14134","EMD-14139"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Chung I","author_orcid":"0000-0002-2902-4677"},{"name":"Wright JJ","author_orcid":"0000-0001-8987-4449"},{"name":"Bridges HR","author_orcid":"0000-0001-6890-6050"},{"name":"Ivanov BS","author_orcid":"0000-0002-1039-3894"},{"name":"Biner O","author_orcid":null},{"name":"Pereira CS","author_orcid":"0000-0002-0248-0883"},{"name":"Arantes GM","author_orcid":"0000-0001-5356-7703"},{"name":"Hirst J","author_orcid":"0000-0001-8667-6797"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Cryo-EM structures define ubiquinone-10 binding to mitochondrial complex I and conformational transitions accompanying Q-site occupancy","volume":"13","country":"","first_page":null,"last_page":null,"year":"2022","language":null,"doi":"10.1038/s41467-022-30506-1","pubmedid":"35589726","details":"Unaligned multi-frame micrographs of Bos taurus mitochondrial complex I from an as-prepared sample solubilised in DDM and reconstituted in to MSP2N2 nanodiscs. Frozen on 0.6/1 UltrAuFoil grids (though holes were larger). The grid was treated with a PEG-thiol reagent (11-mercaptoundecyl hexaethyleneglycol) beforehand to increase hydrophilicity. Images (TIFF) were collected with a 300 keV FEI Titan Krios on a Falcon K3 detector, using counting mode with super-resolution mode and a Gatan energy filter (20 eV). The C2 and objective lens apertures were set to 70 and 100 µm, respectively. A defocus range of -1.0 to -2.4 was used. 2.4 s exposures were captured at a dose rate of 16.9 electrons Å−2 s−1 in 40 frames with a total dose of 40.5 electrons Å−2. A pixel size of 0.535 Å/pixel (super resolution; nominal pixel size was 1.07 Å/pixel) was used.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature Communications","journal_abbreviation":"Nat. Commun.","issue":"1","preprint":false}],"dataset_size":"3.0 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["7qso","7qsl","7qsk","7qsm","7qsn"],"entry_doi":"10.6019/EMPIAR-13132"}}