{"EMPIAR-13093":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of an engineered bEBP-proteasome complex (0 degree tilt)","directory":"data/rawdata_0deg","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":503,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":1.5,"pixel_height":1.5,"micrographs_file_pattern":"data/rawdata_0deg/AM51-4_*.tif","picked_particles_file_pattern":"data/particle_stacks/*.star","picked_particles_directory":"data/particle_images_0deg_J71","details":"Raw micrographs were collected on a Talos Arctica at 200kV equipped with a Gatan K2 camera. Recordings were performed in counting mode at a pixel size of 1.5 A/px with a dose rate of 8.4 electrons/px/s for an exposure time of 12 seconds in dose-fraction mode of 0.3 seconds per-frame resulting in a total of 40 frames and 1.12 electrons/A^2/frame (total dose of 44.8 electrons/A^2). Defocus range for the collection was -0.8 to -3.0 microns. The tilt angle of the specimen was 0 degrees.","image_width":"3710","image_height":"3838"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of an engineered bEBP-proteasome complex (20 degree tilt)","directory":"data/rawdata_20deg","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":466,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":1.5,"pixel_height":1.5,"micrographs_file_pattern":"data/rawdata_20deg/AM52-2_*.tif","picked_particles_file_pattern":"data/particle_stacks/*.star","picked_particles_directory":"data/particle_images_20deg_J66","details":"Raw micrographs were collected on a Talos Arctica at 200kV equipped with a Gatan K2 camera. Recordings were performed in counting mode at a pixel size of 1.5 A/px with a dose rate of 9.2 electrons/px/s for an exposure time of 12 seconds in dose-fraction mode of 0.3 seconds per-frame resulting in a total of 40 frames and 1.227 electrons/A^2/frame (total dose of 49.1 electrons/A^2). Defocus range for the collection was -1 to -2.5 microns. The tilt angle of the specimen was 20 degrees.","image_width":"3710","image_height":"3838"},{"segmentations":[],"name":"Aligned single-frame micrographs of an engineered bEBP-proteasome complex (0 degree tilt)","directory":"data/motioncor_0deg/job002/tiffs","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":503,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":1.5,"pixel_height":1.5,"micrographs_file_pattern":"data/motioncor_0deg/job002/tiffs/AM51-4_*.mrc","picked_particles_file_pattern":"data/particle_stacks/*.star","picked_particles_directory":"data/particle_images_0deg_J71","details":"0 degree tilt data. Raw movies of were aligned using MotionCor2 (through Relion) with a binning factor of 1 and a dose of 1.12 electrons/A^2/frame. Full job output is provided in the upstream directory.","image_width":"3710","image_height":"3838"},{"segmentations":[],"name":"Aligned single-frame micrographs of an engineered bEBP-proteasome complex (20 degree tilt)","directory":"data/motioncor_20deg/job002/tiffs","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":466,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":1.5,"pixel_height":1.5,"micrographs_file_pattern":"data/motioncor_20deg/job002/tiffs/AM52-2_*.mrc","picked_particles_file_pattern":"data/particle_stacks/*.star","picked_particles_directory":"data/particle_images_20deg_J66","details":"20 degree tilt data. Raw movies of were aligned using MotionCor2 (through Relion) with a binning factor of 1 and a dose of 1.227 electrons/A^2/frame. Full job output is provided in the upstream directory.","image_width":"3710","image_height":"3838"},{"segmentations":[],"name":"Particle images of an engineered bEBP-proteasome complex (0 degree tilt)","directory":"data/particle_images_0deg_J71","category":"picked particles - single frame - processed","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":308092,"frames_per_image":0,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":1.5,"pixel_height":1.5,"micrographs_file_pattern":"data/particle_images_0deg_J71/*_AM51-4_*_particles.mrc","picked_particles_file_pattern":"data/particle_stacks/*.star","picked_particles_directory":"data/particle_images_0deg_J71","details":"0 degree tilt data. Number of frames per file is variable (0 was chosen as placeholder). Number of images indicates the total number of particle images across all files.","image_width":"288","image_height":"288"},{"segmentations":[],"name":"Particle images of an engineered bEBP-proteasome complex (20 degree tilt)","directory":"data/particle_images_20deg_J66","category":"picked particles - single frame - processed","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":283067,"frames_per_image":0,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":1.5,"pixel_height":1.5,"micrographs_file_pattern":"data/particle_images_20deg_J66/*_AM52-2_*_particles.mrc","picked_particles_file_pattern":"data/particle_stacks/*.star","picked_particles_directory":"data/particle_images_20deg_J66","details":"20 degree tilt data. Number of frames per file is variable (0 was chosen as placeholder). Number of images indicates the total number of particle images across all files.","image_width":"288","image_height":"288"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)","code":"R35 GM118130","country":"United States"}],"version_history":[],"title":"CryoEM of mycobacterial open-gate proteasome complexed with engineered bacterial enhancer binding protein (bEBP)","principal_investigator":[{"author_orcid":"0000-0002-8241-3153","middle_name":null,"organization":"The Rockefeller University","street":"1230 York Avenue","town_or_city":"New York","state_or_province":"New York","post_or_zip":"10065","telephone":null,"fax":null,"first_name":"Seth","last_name":"Darst","email":"darst [at] rockefeller.edu","country":"United States","entry":"EMPIAR-13093"}],"status":"REL","deposition_date":"2025-10-14","release_date":"2025-12-05","obsolete_date":null,"update_date":"2025-12-05","corresponding_author":{"author":{"author_orcid":"0000-0002-8241-3153","middle_name":null,"organization":"The Rockefeller University","street":"1230 York Avenue","town_or_city":"New York","state_or_province":"New York","post_or_zip":"10065","first_name":"Seth","last_name":"Darst","country":"United States"}},"authors":[{"author":{"name":"Mueller AU","author_orcid":"0000-0003-3002-3695"}},{"author":{"name":"Molina N","author_orcid":"0009-0007-9006-214X"}},{"author":{"name":"Nixon BT","author_orcid":null}},{"author":{"name":"Darst SA","author_orcid":"0000-0002-8241-3153"}}],"cross_references":["EMD-48574"],"biostudies_references":[],"idr_references":[],"empiar_references":[{"name":"EMPIAR-13091"}],"citation":[{"authors":[{"name":"Mueller AU","author_orcid":"0000-0003-3002-3695"},{"name":"Molina N","author_orcid":"0009-0007-9006-214X"},{"name":"Nixon BT","author_orcid":null},{"name":"Darst SA","author_orcid":"0000-0002-8241-3153"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Real-time capture of σN transcription initiation intermediates reveals mechanism of ATPase-driven activation by limited unfolding","volume":"16","country":"","first_page":null,"last_page":null,"year":"2025","language":"English","doi":"10.1038/s41467-025-61837-4","pubmedid":"40759887","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature communications","journal_abbreviation":"Nat Commun","issue":"1","preprint":false}],"dataset_size":"479.4 GB","experiment_type":"EMDB","scale":"molecule","entry_doi":"10.6019/EMPIAR-13093"}}