{"EMPIAR-13047":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of SSU processome assembly intermediates - Dataset1 Kre33 pulldown","directory":"data/Dataset1_Kre33pulldown_tiffs","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":44272,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.54,"pixel_height":0.54,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -1 to -2.5 µm, and a total dose of 60.0 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of yeast SSU processome assembly intermediates - Dataset2 part1 Dhr1 pulldown","directory":"data/Dataset2_part1_Dhr1pulldown_tiffs","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":36229,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.54,"pixel_height":0.54,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -1 to -2.5 µm, and a total dose of 61.6 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of yeast SSU processome assembly intermediates - Dataset2 part2 Dhr1 pulldown","directory":"data/Dataset2_part2_Dhr1pulldown_tiffs","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":48234,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.54,"pixel_height":0.54,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -1 to -2.5 µm, and a total dose of 61.6 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of yeast SSU processome assembly intermediates - Dataset3 - Upt14 pulldown","directory":"data/Dataset3_Upt14pulldown_tiffs","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":85031,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.54,"pixel_height":0.54,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -1 to -2.5 µm, and a total dose of 61.6 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Polished particle stacks of yeast SSU processome assembly intermediates- State A to State O","directory":"data/Particle_stacks","category":"micrographs - single frame","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":801888,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.063,"pixel_height":1.063,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Particles stacks from the four datasets for each state (State A to State O). Particles are polished, magnification anisotropy corrected and per-particle defocus corrected. A Relion .star format file is provided and contains the final Euler angles, shifts and also the micrograph coordinates.","image_width":"480","image_height":"480"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health (NIH)","code":"R01GM145950","country":"United States"},{"funding_body":"National Institutes of Health (NIH)","code":"R35GM156426","country":"United States"},{"funding_body":"Chan Zuckerberg Initiative","code":"Exploratory Cell Network","country":"United States"}],"version_history":[],"title":"Single particle cryo-EM dataset of yeast SSU processome assembly intermediates","principal_investigator":[{"author_orcid":"0000-0002-9373-4737","middle_name":null,"organization":"Laboratory of Protein and Nucleic Acid Chemistry, Rockefeller University","street":null,"town_or_city":"New York","state_or_province":null,"post_or_zip":"10065","telephone":null,"fax":null,"first_name":"Sebastian","last_name":"Klinge","email":"sklinge [at] rockefeller.edu","country":"United States","entry":"EMPIAR-13047"}],"status":"REL","deposition_date":"2025-09-18","release_date":"2025-11-16","obsolete_date":null,"update_date":"2025-11-16","corresponding_author":{"author":{"author_orcid":"0000-0002-9373-4737","middle_name":null,"organization":"Laboratory of Protein and Nucleic Acid Chemistry, Rockefeller University","street":"1230 York Avenue","town_or_city":"New York","state_or_province":"New York","post_or_zip":"10065","first_name":"Sebastian","last_name":"Klinge","country":"United States"}},"authors":[{"author":{"name":"Buzovetsky OB","author_orcid":"0000-0002-4934-6059"}}],"cross_references":["EMD-49085","EMD-49079","EMD-49076","EMD-49075","EMD-49086","EMD-49087","EMD-49077","EMD-49080","EMD-49089","EMD-49082"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Buzovetsky OB","author_orcid":"0000-0002-4934-6059"},{"name":"Klinge SK","author_orcid":"0000-0002-9373-4737"}],"editors":[],"published":false,"j_or_nj_citation":true,"title":"Helicase-mediated mechanism of SSU processome maturation and disassembly","volume":null,"country":"","first_page":null,"last_page":null,"year":null,"language":"english","doi":null,"pubmedid":null,"details":"Raw micrographs","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature","journal_abbreviation":"Nature","issue":null,"preprint":true}],"dataset_size":"187.0 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9n75","9n6z","9n6w","9n6v","9n76","9n77","9n6x","9n70","9n79","9n72"],"entry_doi":"10.6019/EMPIAR-13047"}}