{"EMPIAR-12962":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame movies of PbAbiF RNP","directory":"data/Micrographs","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":4272,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.663,"pixel_height":0.663,"micrographs_file_pattern":"data/Micrographs/Images-Disc1/GridSquare_*/Data/FoilHole_*_fractions.tiff","picked_particles_file_pattern":"data/particles.star","picked_particles_directory":"","details":"Data collected at MIT.nano on Thermo Scientific Titan Krios G3i cryo-TEM\n\nVoltage\t\t300 keV\nCamera\t\tGatan K3, super-resolution mode with 2-fold binning by EPU\nEnergy filter\tGIF, 20eV slit width\nMagnification\t130000\nPixel size\t0.663 A/pix\nDose\t\t42.2 e/A2\nExposure rate\t18.5 e/pix/s\nExposure time\t1.00 s\nFractions\t40\n\nTotal mics\t4272\n\n\nGainReference.mrc was binned using 'rebin by two' in Digital Micrograph. It needs to be flipped for use in RELION motion correction (Gain flip: Flip upside down (1) ). It can probably be used as is for cryoSPARC.\n\nparticles.star contains metadata for the particle selected for the final reconstruction.","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo-EM micrographs of PbAbiF dimer bound to ncRNA","principal_investigator":[{"author_orcid":"0000-0003-0178-7995","middle_name":null,"organization":"Massachusetts Institute of Technology","street":null,"town_or_city":"Cambridge","state_or_province":"Massachusetts","post_or_zip":"02142","telephone":null,"fax":null,"first_name":"Feng","last_name":"Zhang","email":"zhang [at] mit.edu","country":"United States","entry":"EMPIAR-12962"}],"status":"REL","deposition_date":"2025-09-09","release_date":"2025-12-07","obsolete_date":null,"update_date":"2025-12-07","corresponding_author":{"author":{"author_orcid":"0000-0003-4738-9503","middle_name":null,"organization":"Massachusetts Institute of Technology","street":null,"town_or_city":"Cambridge","state_or_province":"Massachusetts","post_or_zip":"02142","first_name":"Max","last_name":"Wilkinson","country":"United States"}},"authors":[{"author":{"name":"Wilkinson ME","author_orcid":"0000-0003-4738-9503"}},{"author":{"name":"Zhang F","author_orcid":"0000-0003-0178-7995"}}],"cross_references":["EMD-43682"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Zilberzwige-Tal S","author_orcid":null},{"name":"Altae-Tran H","author_orcid":null},{"name":"Kannan S","author_orcid":null},{"name":"Wilkinson ME","author_orcid":null},{"name":"Vo SC","author_orcid":null},{"name":"Strebinger D","author_orcid":null},{"name":"Edmonds KK","author_orcid":null},{"name":"Yao CJ","author_orcid":null},{"name":"Mears KS","author_orcid":null},{"name":"Shmakov SA","author_orcid":null},{"name":"Makarova KS","author_orcid":null},{"name":"Macrae RK","author_orcid":null},{"name":"Koonin EV","author_orcid":null},{"name":"Zhang F","author_orcid":null}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Reprogrammable RNA-targeting CRISPR systems evolved from RNA toxin-antitoxins","volume":"188","country":"","first_page":"1925","last_page":"1940.e20","year":"2025","language":"English","doi":"10.1016/j.cell.2025.01.034","pubmedid":"39970912","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Cell","journal_abbreviation":"Cell","issue":"7","preprint":false}],"dataset_size":"587.8 GB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["8vz6"],"entry_doi":"10.6019/EMPIAR-12962"}}