{"EMPIAR-12960":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame movies of Taeniopygia guttata R2 retrotransposon initiating target-primed reverse transcription","directory":"data/Krios_20240308_TgD4","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":10628,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.663,"pixel_height":0.663,"micrographs_file_pattern":"data/Krios_20240308_TgD4/Images-Disc1/GridSquare_*/Data/FoilHole_*_fractions.tiff","picked_particles_file_pattern":"data/particles.star","picked_particles_directory":"","details":"RAW MICROGRAPHS AND REFINEMENT STAR FILE FOR R2Tg TPRT COMPLEX (EMD-47091; PDB 9DOU)\nData collected at MIT.nano on Thermo Scientific Titan Krios G3i cryo-TEM\n\nVoltage\t\t300 keV\nCamera\t\tGatan K3, super-resolution mode with 2-fold binning by EPU\nEnergy filter\tGIF, 20eV slit width\nMagnification\t130000\nPixel size\t0.663 A/pix\nDose\t\t59.6 e/A2\nExposure rate\t25.7 e/pix/s\nExposure time\t1.02 s\nFractions\t40\n\nTotal mics\t10628\n\n\nGainReference.mrc was binned using 'rebin by two' in Digital Micrograph. It needs to be flipped for use in RELION motion correction (Gain flip: Flip upside down (1) ). It can probably be used as is for cryoSPARC.\n\nparticles.star contains metadata for the particle selected for the final reconstruction.","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo-EM micrographs of R2Tg retrotransposon initiating target-primed reverse transcription","principal_investigator":[{"author_orcid":"0000-0003-0178-7995","middle_name":null,"organization":"Broad Institute of Harvard and MIT","street":"75 Ames Street","town_or_city":"Cambridge","state_or_province":"Massachusetts","post_or_zip":"02142","telephone":"6177147000","fax":null,"first_name":"Feng","last_name":"Zhang","email":"zhang [at] broadinstitute.org","country":"United States","entry":"EMPIAR-12960"}],"status":"REL","deposition_date":"2025-09-08","release_date":"2025-11-30","obsolete_date":null,"update_date":"2025-11-30","corresponding_author":{"author":{"author_orcid":"0000-0003-4738-9503","middle_name":null,"organization":"Massachusetts Institute of Technology","street":"75 Ames Street","town_or_city":"Cambridge","state_or_province":"Massachusetts","post_or_zip":"02142","first_name":"Max","last_name":"Wilkinson","country":"United States"}},"authors":[{"author":{"name":"Wilkinson ME","author_orcid":"0000-0003-4738-9503"}},{"author":{"name":"Zhang F","author_orcid":"0000-0003-0178-7995"}}],"cross_references":["EMD-47091"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Edmonds KK","author_orcid":"0000-0002-7317-2669"},{"name":"Wilkinson ME","author_orcid":"0000-0003-4738-9503"},{"name":"Strebinger D","author_orcid":"0000-0001-8732-6414"},{"name":"Chen H","author_orcid":"0000-0002-9496-8586"},{"name":"Lash B","author_orcid":"0000-0003-2445-7948"},{"name":"Schaefer CC","author_orcid":"0009-0005-3701-913X"},{"name":"Zhu S","author_orcid":"0009-0001-2677-8262"},{"name":"Liu D","author_orcid":"0000-0001-6598-8871"},{"name":"Zilberzwige-Tal S","author_orcid":"0000-0001-9223-5082"},{"name":"Ladha A","author_orcid":"0000-0003-3945-7924"},{"name":"Walsh ML","author_orcid":"0000-0001-5563-5829"},{"name":"Frangieh CJ","author_orcid":null},{"name":"Vaz Reay NA","author_orcid":null},{"name":"Macrae RK","author_orcid":"0000-0002-7850-9056"},{"name":"Wang X","author_orcid":"0000-0002-3090-9894"},{"name":"Zhang F","author_orcid":"0000-0003-0178-7995"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structure and biochemistry-guided engineering of an all-RNA system for DNA insertion with R2 retrotransposons","volume":"16","country":"United States","first_page":null,"last_page":null,"year":"2025","language":"English","doi":"10.1038/s41467-025-61321-z","pubmedid":"40603868","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature communications","journal_abbreviation":"Nat Commun","issue":"1","preprint":false}],"dataset_size":"1.7 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9dou"],"entry_doi":"10.6019/EMPIAR-12960"}}