{"EMPIAR-12954":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of the human mortalin(R126W)-GrpEL1(Y173A) complex - dataset 1","directory":"data/Movies_1","category":"micrographs - multiframe","header_format":"EER","data_format":"EER","num_images_or_tilt_series":402,"frames_per_image":1302,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.735,"pixel_height":0.735,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned multi-frame micrographs of the human mortalin(R126W)-GrpEL1(Y173A) complex collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon-4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2. \n\nGain file required for motion correction of unaligned micrographs for dataset 1 is included.","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of the human mortalin(R126W)-GrpEL1(Y173A) complex - dataset 2","directory":"data/Movies_2","category":"micrographs - multiframe","header_format":"EER","data_format":"EER","num_images_or_tilt_series":1995,"frames_per_image":1267,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.735,"pixel_height":0.735,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned multi-frame micrographs of the human mortalin(R126W)-GrpEL1(Y173A) complex collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon-4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2. \n\nGain file required for motion correction of unaligned micrographs for dataset 2 is included.","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of the human mortalin(R126W)-GrpEL1(Y173A) complex - dataset 3","directory":"data/Movies_3","category":"micrographs - multiframe","header_format":"EER","data_format":"EER","num_images_or_tilt_series":1272,"frames_per_image":1400,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.735,"pixel_height":0.735,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned multi-frame micrographs of the human mortalin(R126W)-GrpEL1(Y173A) complex collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon-4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2. \n\nGain file required for motion correction of unaligned micrographs for dataset 3 is included.","image_width":"4096","image_height":"4096"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health (NIH)","code":"R35-GM138206","country":"United States"},{"funding_body":"National Institutes of Health (NIH)","code":"T32-GM008326","country":"United States"},{"funding_body":"Kinship Foundation","code":"Searle Scholars Program","country":"United States"}],"version_history":[],"title":"CryoEM movies of the human mortalin(R126W)-GrpEL1(Y173A) complex","principal_investigator":[{"author_orcid":"0000-0001-6653-6682","middle_name":"A","organization":"Department of Chemistry and Biochemistry, University of California San Diego","street":"9500 Gilman Drive","town_or_city":"La Jolla","state_or_province":"California","post_or_zip":"92093","telephone":"858 246 5875","fax":null,"first_name":"Mark","last_name":"Herzik Jr.","email":"mherzik [at] ucsd.edu","country":"United States","entry":"EMPIAR-12954"}],"status":"REL","deposition_date":"2025-08-29","release_date":"2025-09-17","obsolete_date":null,"update_date":"2025-09-17","corresponding_author":{"author":{"author_orcid":"0000-0001-6653-6682","middle_name":"A","organization":"Department of Chemistry and Biochemistry, University of California San Diego","street":"9500 Gilman Drive","town_or_city":"La Jolla","state_or_province":"California","post_or_zip":"92093","first_name":"Mark","last_name":"Herzik Jr.","country":"United States"}},"authors":[{"author":{"name":"Morizono MA","author_orcid":"0000-0003-2261-4452"}},{"author":{"name":"Herzik Jr MA","author_orcid":"0000-0001-6653-6682"}}],"cross_references":["EMD-44676","EMD-44677"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Morizono MA","author_orcid":"0000-0003-2261-4452"},{"name":"McGuire KL","author_orcid":null},{"name":"Birouty NI","author_orcid":null},{"name":"Herzik MA","author_orcid":"0000-0001-6653-6682"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structural insights into GrpEL1-mediated nucleotide and substrate release of human mitochondrial Hsp70","volume":"15","country":"","first_page":"10815","last_page":"10815","year":"2024","language":"English","doi":"10.1038/s41467-024-54499-1","pubmedid":"39737924","details":"CryoEM movies of the human mortalin(R126W)-GrpEL1(Y173A) complex. Data were collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon 4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2 with a defocus range of − 1.0 to − 2.5 µm. Data were collected automatically using EPU with aberration free image shift.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature communications","journal_abbreviation":"Nat Commun","issue":"1","preprint":false}],"dataset_size":"2.4 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9blt","9blu"],"entry_doi":"10.6019/EMPIAR-12954"}}