{"EMPIAR-12928":{"imagesets":[{"segmentations":[],"name":"1/5 Unaligned multi-frame micrographs of the Cas1-2/3 heterohexameric complex bound to a 34 bp fragment of dsDNA with a PAM and to a dsDNA fragment containing the CRISPR leader, repeat, and spacer","directory":"data/rawdata1-pixelsize-1152","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":1756,"frames_per_image":50,"frame_range_min":1,"frame_range_max":50,"voxel_type":"SIGNED BYTE","pixel_width":1.152,"pixel_height":1.152,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Movies were acquired using a Gatan K3 direct electron detector, operated in electron counting mode targeting a total electron exposure of 65.67 e-/Å2 over 50 frames (5.992 second exposure, 0.12 second frame time). The SerialEM data collection software was used to collect micrographs at 36,000x nominal magnification (1.152 Å/pixel at the specimen level) over a defocus range of -0.5 µm to -2.0 µm. Movies were gain-corrected on the microscope server so no gain reference is provided.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"2/5 Unaligned multi-frame micrographs of the Cas1-2/3 heterohexameric complex bound to a 34 bp fragment of dsDNA with a PAM and to a dsDNA fragment containing the CRISPR leader, repeat, and spacer","directory":"data/rawdata2-pixelsize-1152","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":1841,"frames_per_image":50,"frame_range_min":1,"frame_range_max":50,"voxel_type":"SIGNED BYTE","pixel_width":1.152,"pixel_height":1.152,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Movies were acquired using a Gatan K3 direct electron detector, operated in electron counting mode targeting a total electron exposure of 65.67 e-/Å2 over 50 frames (5.992 second exposure, 0.12 second frame time). The SerialEM data collection software was used to collect micrographs at 36,000x nominal magnification (1.152 Å/pixel at the specimen level) over a defocus range of -0.5 µm to -2.0 µm. Movies were gain-corrected on the microscope server so no gain reference is provided.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"3/5 Unaligned multi-frame micrographs of the Cas1-2/3 heterohexameric complex bound to a 34 bp fragment of dsDNA with a PAM and to a dsDNA fragment containing the CRISPR leader, repeat, and spacer","directory":"data/rawdata3-gr5-pixelsize-1152","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":3759,"frames_per_image":50,"frame_range_min":1,"frame_range_max":50,"voxel_type":"SIGNED BYTE","pixel_width":1.152,"pixel_height":1.152,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Movies were acquired using a Gatan K3 direct electron detector, operated in electron counting mode targeting a total electron exposure of 65.67 e-/Å2 over 50 frames (5.992 second exposure, 0.12 second frame time). The SerialEM data collection software was used to collect micrographs at 36,000x nominal magnification (1.152 Å/pixel at the specimen level) over a defocus range of -0.5 µm to -2.0 µm. Movies were gain-corrected on the microscope server so no gain reference is provided.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"4/5 Unaligned multi-frame micrographs of the Cas1-2/3 heterohexameric complex bound to a 34 bp fragment of dsDNA with a PAM and to a dsDNA fragment containing the CRISPR leader, repeat, and spacer","directory":"data/rawdata4-gr5-pixelsize-1152","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":2055,"frames_per_image":50,"frame_range_min":1,"frame_range_max":50,"voxel_type":"SIGNED BYTE","pixel_width":1.152,"pixel_height":1.152,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Movies were acquired using a Gatan K3 direct electron detector, operated in electron counting mode targeting a total electron exposure of 65.67 e-/Å2 over 50 frames (5.992 second exposure, 0.12 second frame time). The SerialEM data collection software was used to collect micrographs at 36,000x nominal magnification (1.152 Å/pixel at the specimen level) over a defocus range of -0.5 µm to -2.0 µm. Movies were gain-corrected on the microscope server so no gain reference is provided.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"5/5 Unaligned multi-frame micrographs of the Cas1-2/3 heterohexameric complex bound to a 34 bp fragment of dsDNA with a PAM and to a dsDNA fragment containing the CRISPR leader, repeat, and spacer","directory":"data/rawdata5-SR-pixelsize-0576","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":995,"frames_per_image":50,"frame_range_min":1,"frame_range_max":50,"voxel_type":"SIGNED BYTE","pixel_width":0.576,"pixel_height":0.576,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Movies were acquired using a Gatan K3 direct electron detector, operated in electron counting mode targeting a total electron exposure of 65.67 e-/Å2 over 50 frames (5.992 second exposure, 0.12 second frame time). The SerialEM data collection software was used to collect micrographs at 36,000x nominal magnification (1.152 Å/pixel at the specimen level, collected in super-resolution mode with a physical pixel size of 0.576 A/pixel.) over a defocus range of -0.5 µm to -2.0 µm. Movies were gain-corrected on the microscope server so no gain reference is provided.","image_width":"11520","image_height":"8184"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cas1-2/3 integration complex, 34 bp foreign DNA with PAM, CRISPR leader-repeat-spacer DNA fragment","principal_investigator":[{"author_orcid":"0000-0001-9297-5304","middle_name":null,"organization":"Department of Microbiology and Cellular Biology, Montana State University, Bozeman, MT","street":"128 Cooley Laboratory","town_or_city":"Bozeman","state_or_province":"Montana","post_or_zip":"59717","telephone":null,"fax":null,"first_name":"Blake","last_name":"Wiedenheft","email":"bwiedenheft [at] gmail.com","country":"United States","entry":"EMPIAR-12928"}],"status":"REL","deposition_date":"2025-08-11","release_date":"2025-11-04","obsolete_date":null,"update_date":"2025-11-04","corresponding_author":{"author":{"author_orcid":"0000-0001-9836-3827","middle_name":null,"organization":"Department of Microbiology and Cell Biology, Montana State University","street":"128 Cooley Laboratory","town_or_city":"Bozeman","state_or_province":"Montana","post_or_zip":"59717","first_name":"William","last_name":"Henriques","country":"United States"}},"authors":[{"author":{"name":"Henriques WS","author_orcid":"0000-0001-9836-3827"}},{"author":{"name":"Bowman J","author_orcid":null}},{"author":{"name":"Hall LN","author_orcid":"0000-0003-3135-332X"}},{"author":{"name":"Gauvin CC","author_orcid":"0000-0001-7171-552X"}},{"author":{"name":"Wei H","author_orcid":"0000-0001-6777-1772"}},{"author":{"name":"Kuang H","author_orcid":"0000-0002-9891-1747"}},{"author":{"name":"Zimanyi CM","author_orcid":"0000-0002-6782-507X"}},{"author":{"name":"Eng ET","author_orcid":"0000-0002-8014-7269"}},{"author":{"name":"Santiago-Frangos A","author_orcid":"0000-0001-9615-065X"}},{"author":{"name":"Wiedenheft B","author_orcid":"0000-0001-9297-5304"}}],"cross_references":["EMD-71101","EMD-71112"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Henriques WS","author_orcid":"0000-0001-9836-3827"},{"name":"Bowman J","author_orcid":null},{"name":"Hall LN","author_orcid":"0000-0003-3135-332X"},{"name":"Gauvin CC","author_orcid":"0000-0001-7171-552X"},{"name":"Wei H","author_orcid":"0000-0001-6777-1772"},{"name":"Kuang H","author_orcid":"0000-0002-9891-1747"},{"name":"Zimanyi CM","author_orcid":"0000-0002-6782-507X"},{"name":"Eng ET","author_orcid":"0000-0002-8014-7269"},{"name":"Santiago-Frangos A","author_orcid":"0000-0001-9615-065X"},{"name":"Wiedenheft B","author_orcid":"0000-0001-9297-5304"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structures reveal how the Cas1-2/3 integrase captures, delivers, and integrates foreign DNA into CRISPR loci","volume":null,"country":"United States","first_page":null,"last_page":null,"year":"2025","language":"English","doi":"10.1101/2025.06.10.658980","pubmedid":"40661357","details":"40 frame movies were collected at a 150 ms frame rate and a dose rate of 8.63 e-/Å2/s for a total dose of 51.78 e-/Å2 with a pixel size of 0.833 Å/px over a defocus range of -0.9 to -2.8 um.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"bioRxiv","journal_abbreviation":"","issue":null,"preprint":true},{"authors":[{"name":"Henriques WS","author_orcid":null},{"name":"Bowman J","author_orcid":null},{"name":"Hall LN","author_orcid":null},{"name":"Gauvin CC","author_orcid":null},{"name":"Wei H","author_orcid":null},{"name":"Kuang H","author_orcid":null},{"name":"Zimanyi CM","author_orcid":null},{"name":"Eng ET","author_orcid":null},{"name":"Santiago-Frangos A","author_orcid":null},{"name":"Wiedenheft B","author_orcid":null}],"editors":[{"name":" ","author_orcid":null}],"published":true,"j_or_nj_citation":true,"title":"Structures reveal how the Cas1-2/3 integrase captures, delivers, and integrates foreign DNA into CRISPR loci","volume":null,"country":"","first_page":null,"last_page":null,"year":"2025","language":"English","doi":"10.1016/j.str.2025.09.007","pubmedid":"41072406","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Structure (London, England : 1993)","journal_abbreviation":"Structure","issue":null,"preprint":false}],"dataset_size":"9.1 TB","experiment_type":"EMDB","scale":"molecule","entry_doi":"10.6019/EMPIAR-12928"}}