{"EMPIAR-12904":{"imagesets":[{"segmentations":[],"name":"Unaligned eer micrographs of WT M mazei Top6-327 bp minicircle DNA-ADPNP complex","directory":"data","category":"micrographs - multiframe","header_format":"EER","data_format":"EER","num_images_or_tilt_series":50906,"frames_per_image":0,"frame_range_min":null,"frame_range_max":null,"voxel_type":"BIT","pixel_width":0.959,"pixel_height":0.959,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"~80% of this dataset was collected at 30 degree stage tilt (n23feb, may, jun, jul folders). The n23jan folder was collected at 0 degree tilt. Data were collected at National Center for CryoEM Access and Training (New York Structural Biology Center) on Krios 7 \"Matthew\", camera: TFS Falcon IV, image shift at 130kx mag, exposure settings: 7.0 s [images saved in eer format], e flux: ~6 e-/px/s with ~50 e-/Å total dose, pixel size: ~0.959Å/px (nominal) [0.926Å(calibrated against apoF)] (e- counting with an image dimension of 4096x4096 px), energy filter: Selectris 10-20 eV, defocus range: -0.8 µm to -2.5 µm (nominal).","image_width":"4096","image_height":"4096"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health/National Cancer Institute (NIH/NCI)","code":"5R35 CA263778","country":"United States"},{"funding_body":"National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)","code":"1F32 GM128269","country":"United States"}],"version_history":[],"title":"CryoEM dataset of M. mazei topoisomerase VI-minicircle DNA complex","principal_investigator":[{"author_orcid":"0000-0003-0666-1240","middle_name":null,"organization":"Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine","street":"725 N Wolfe St","town_or_city":"Baltimore","state_or_province":"MD","post_or_zip":"21205","telephone":"1 410 955 7163","fax":null,"first_name":"James","last_name":"Berger","email":"jmberger [at] jhmi.edu","country":"United States","entry":"EMPIAR-12904"}],"status":"REL","deposition_date":"2025-07-30","release_date":"2026-03-02","obsolete_date":null,"update_date":"2026-04-07","corresponding_author":{"author":{"author_orcid":"0000-0002-3602-5651","middle_name":null,"organization":"Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine","street":"725 N Wolfe St","town_or_city":"Baltimore","state_or_province":"MD","post_or_zip":"21205","first_name":"Daniel","last_name":"Richman","country":"United States"}},"authors":[{"author":{"name":"Richman DE","author_orcid":"0000-0002-3602-5651"}},{"author":{"name":"Berger JM","author_orcid":"0000-0003-0666-1240"}}],"cross_references":["EMD-70259","EMD-70239","EMD-70232"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Richman DE","author_orcid":"0000-0002-3602-5651"},{"name":"Wendorff TJ","author_orcid":"0000-0002-3729-4706"},{"name":"Rashid F","author_orcid":"0000-0003-3898-3842"},{"name":"Beck C","author_orcid":"0000-0002-6674-9802"},{"name":"Yan Q","author_orcid":null},{"name":"Johnson HR","author_orcid":"0009-0000-3444-1910"},{"name":"Eckerty RA","author_orcid":null},{"name":"Fogg JM","author_orcid":"0000-0003-1553-9669"},{"name":"Baker ML","author_orcid":"0000-0001-9039-8523"},{"name":"Zechiedrich L","author_orcid":"0000-0003-0533-1637"},{"name":"Berger JM","author_orcid":"0000-0003-0666-1240"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Supercoiled DNA recognition and cleavage control in topoisomerase VI","volume":"17","country":"","first_page":null,"last_page":null,"year":"2026","language":"English","doi":"10.1038/s41467-026-69491-0","pubmedid":"41698922","details":"These micrographs are unaligned eer files combined from six separate sessions on the same microscope with the same settings and identically prepared grids.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature communications","journal_abbreviation":"Nat Commun","issue":"1","preprint":false}],"dataset_size":"29.6 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9o9m","9o8z","9o8p"],"entry_doi":"10.6019/EMPIAR-12904"}}