{"EMPIAR-12886":{"imagesets":[{"segmentations":[],"name":"unaligned multiple frame raw micrographs for h3.v h4.v ncp","directory":"data/Raw_lzw_tiff","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":8052,"frames_per_image":50,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.53,"pixel_height":0.53,"micrographs_file_pattern":"data/Raw_lzw_tiff/*.tiff","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Gatan K3, Titan Krios, GIF energy filter, operating at 83,000x magnification. Data collected in superresolution mode, gain (estimated from data) and binning applied in motioncorrection processing step. Dose rate (e-/pixel/s) 13.89, total accumulated dose 54.4","image_width":"11520","image_height":"8184"}],"workflow_file":null,"grant_references":[{"funding_body":"Wellcome Trust","code":"210493/Z/18/Z","country":"United Kingdom"},{"funding_body":"Biotechnology and Biological Sciences Research Council (BBSRC)","code":"BB/M010996/1","country":"United Kingdom"}],"version_history":[],"title":"Cryo-EM structure of H3.V H4.V trypanosome nucleosome","principal_investigator":[{"author_orcid":"0000-0001-9551-5514","middle_name":"David","organization":"University of Edinburgh","street":"Michael swann building","town_or_city":"edinburgh","state_or_province":"Lothian","post_or_zip":"eh9 3jr","telephone":"44131657029","fax":null,"first_name":"marcus","last_name":"wilson","email":"marcus.wilson [at] ed.ac.uk","country":"United Kingdom","entry":"EMPIAR-12886"}],"status":"REL","deposition_date":"2025-07-09","release_date":"2026-05-27","obsolete_date":null,"update_date":"2026-05-27","corresponding_author":{"author":{"author_orcid":"0000-0001-9551-5514","middle_name":"David","organization":"University of Edinburgh","street":"Michael swann building","town_or_city":"edinburgh","state_or_province":"Lothian","post_or_zip":"eh9 3jr","first_name":"Marcus","last_name":"wilson","country":"United Kingdom"}},"authors":[{"author":{"name":"Deák G","author_orcid":"0000-0001-6296-9384"}},{"author":{"name":"Wilson MD","author_orcid":"0000-0001-9551-5514"}}],"cross_references":["EMD-53505"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Deák G","author_orcid":"0000-0001-6296-9384"},{"name":"Burdett H","author_orcid":"0000-0003-4803-9660"},{"name":"Watson JA","author_orcid":"0000-0002-5691-1829"},{"name":"Wilson MD","author_orcid":"0000-0001-9551-5514"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Trypanosome histone variants H3.V and H4.V promote nucleosome plasticity in repressed chromatin","volume":"34","country":"","first_page":"629","last_page":"644.e10","year":"2026","language":"English","doi":"10.1016/j.str.2026.01.008","pubmedid":"41709455","details":"Data collection was performed on a TFS Titan Krios at 300 kV equipped with a Gatan K3 Bioquantum (6k x 4k) detector. 8052 movies were obtained using EPU software in super-resolution mode using a pixel size of 1.06 Å, target defocus range of -1.5 to -3.0 Å, 4.4s exposure time, and a total dose of 54.4 e- Å-2. 8052 raw non-corrected micrographs were imported into CryoSPARC (Punjani et al., 2017), binned to return to physical pixel size, and normalised using a gain reference estimated from a subset of 1000 micrographs using the relion_estimate_gain command in RELION-3.1","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Structure (London, England : 1993)","journal_abbreviation":"Structure","issue":"4","preprint":false}],"dataset_size":"4.1 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9r1d"],"entry_doi":"10.6019/EMPIAR-12886"}}