{"EMPIAR-12791":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame movies of Cas12j3/crRNA/DNA complex in postcleavage state.","directory":"data/Images-Disc1","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":4445,"frames_per_image":40,"frame_range_min":1,"frame_range_max":4445,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":0.832,"pixel_height":0.832,"micrographs_file_pattern":"data/Images-Disc1/GridSquare_*/Data/*s.mrc","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Movies were collected in a Titan Krios G2 equipped with a TFS Falcon III camera operated at 300 keV in counting mode at a magnification of x 96000 (0.832 A˚ /px) using a total dose of 42 e/A˚ 2. Spherical Aberration: 2.7 mm. Out of the 4445 original micrographs deposited (gain-corrected, non-motion-corrected, 40 fractions), 4393 were used for picking.\n\nMicroscope Settings:\n   -Acceleration Voltage: 300 kV\n   -Pixel Size: 0.832 Å\n   -Spherical Aberration: 2.7 mm\n   -Total Exposure Dose: 42 e/Ų\n   -Number of Fractions: 40","image_width":"4096","image_height":"4096"}],"workflow_file":null,"grant_references":[{"funding_body":"Novo Nordisk Foundation","code":"NNF0024386, NNF17OC0030788, NNF14CC0001, NNF17SA0030214, NNF18OC0055061","country":"Denmark"}],"version_history":[],"title":"CryoEM dataset of Cas12j3 (Casphi3) in complex with crRNA and target DNA after cleavage (postcatalytic state)","principal_investigator":[{"author_orcid":"0000-0002-8269-3941","middle_name":null,"organization":"Novo Nordisk Foundation Center for Protein Research","street":"Blegdamsvej 3B, DK-2200 Copenhagen N","town_or_city":"Copenhagen","state_or_province":"Hovedstaden","post_or_zip":"2200","telephone":null,"fax":null,"first_name":"Guillermo","last_name":"Montoya","email":"guillermo.montoya [at] cpr.ku.dk","country":"Denmark","entry":"EMPIAR-12791"}],"status":"REL","deposition_date":"2025-05-08","release_date":"2025-07-01","obsolete_date":null,"update_date":"2025-07-01","corresponding_author":{"author":{"author_orcid":"0000-0001-6286-2309","middle_name":null,"organization":"Novo Nordisk Foundation Center for Protein Research","street":"Blegdamsvej 3B, DK-2200 Copenhagen N","town_or_city":"Copenhagen","state_or_province":"Hovedstaden","post_or_zip":"2200","first_name":"Arturo","last_name":"Carabias del Rey","country":"Denmark"}},"authors":[{"author":{"name":"Carabias A","author_orcid":"0000-0001-6286-2309"}},{"author":{"name":"Fuglsang A","author_orcid":"0000-0001-8724-6457"}},{"author":{"name":"Temperini P","author_orcid":"0000-0003-4951-6879"}},{"author":{"name":"Pape T","author_orcid":"0000-0002-0612-3568"}},{"author":{"name":"Sofos N","author_orcid":"0000-0002-7224-3330"}},{"author":{"name":"Stella S","author_orcid":"0000-0002-9078-4659"}},{"author":{"name":"Erlendsson S","author_orcid":"0000-0002-6378-870X"}},{"author":{"name":"Montoya G","author_orcid":"0000-0002-8269-3941"}}],"cross_references":["EMD-12827"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Carabias A","author_orcid":"0000-0001-6286-2309"},{"name":"Fuglsang A","author_orcid":"0000-0001-8724-6457"},{"name":"Temperini P","author_orcid":"0000-0003-4951-6879"},{"name":"Pape T","author_orcid":"0000-0002-0612-3568"},{"name":"Sofos N","author_orcid":"0000-0002-7224-3330"},{"name":"Stella S","author_orcid":"0000-0002-9078-4659"},{"name":"Erlendsson S","author_orcid":"0000-0002-6378-870X"},{"name":"Montoya G","author_orcid":"0000-0002-8269-3941"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3","volume":"12","country":"","first_page":"4476","last_page":"na","year":"2021","language":"English","doi":"10.1038/s41467-021-24707-3","pubmedid":"34294706","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature communications","journal_abbreviation":"Nat Commun","issue":"1","preprint":false}],"dataset_size":"5.6 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["7odf"],"entry_doi":"10.6019/EMPIAR-12791"}}