{"EMPIAR-12744":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of human hemoglobin (Hb) in the presence of 5 mM sodium dithionite under Al's oil","directory":"data/Movies","category":"micrographs - multiframe","header_format":"EER","data_format":"EER","num_images_or_tilt_series":1538,"frames_per_image":1939,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.735,"pixel_height":0.735,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned multi-frame micrographs of human hemoglobin (Hb) in the presence of 5 mM sodium dithionite under Al's oil collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2.","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"Aligned micrographs of human hemoglobin (Hb) in the presence of 5 mM sodium dithionite under Al's oil","directory":"data/Micrographs","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1538,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.735,"pixel_height":0.735,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Aligned micrographs of human hemoglobin (Hb) in the presence of 5 mM sodium dithionite under Al's oil collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2.","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"Gain file for motion correction of unaligned micrographs of human hemoglobin (Hb) in the presence of 5 mM sodium dithionite under Al's oil","directory":"data/Gains","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.735,"pixel_height":0.735,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Gain file required for motion correction of unaligned micrographs of human hemoglobin (Hb) in the presence of 5 mM sodium dithionite under Al's oil.","image_width":"4096","image_height":"4096"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health (NIH)","code":"R35-GM138206","country":"United States"},{"funding_body":"National Institutes of Health (NIH)","code":"R01-GM148607","country":"United States"},{"funding_body":"National Institutes of Health (NIH)","code":"1S10OD032471","country":"United States"},{"funding_body":"National Institutes of Health (NIH)","code":"T32-EB009380","country":"United States"}],"version_history":[],"title":"CryoEM movies of human hemoglobin (Hb) in the presence of 5 mM sodium dithionite under Al's oil","principal_investigator":[{"author_orcid":"0000-0001-6653-6682","middle_name":"A","organization":"Department of Chemistry and Biochemistry, University of California, San Diego","street":"9500 Gilman Drive","town_or_city":"La Jolla","state_or_province":"California","post_or_zip":"92093","telephone":"858 246 5875","fax":null,"first_name":"Mark","last_name":"Herzik, Jr","email":"mherzik [at] ucsd.edu","country":"United States","entry":"EMPIAR-12744"}],"status":"REL","deposition_date":"2025-04-28","release_date":"2025-06-30","obsolete_date":null,"update_date":"2025-06-30","corresponding_author":{"author":{"author_orcid":"0000-0001-6653-6682","middle_name":"A","organization":"Department of Chemistry and Biochemistry, University of California, San Diego","street":"9500 Gilman Drive","town_or_city":"La Jolla","state_or_province":"California","post_or_zip":"92093","first_name":"Mark","last_name":"Herzik, Jr","country":"United States"}},"authors":[{"author":{"name":"Cook BD","author_orcid":"0000-0002-5777-3464"}},{"author":{"name":"Herzik Jr MA","author_orcid":"0000-0001-6653-6682"}}],"cross_references":["EMD-45821","EMD-45822"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Cook BD","author_orcid":"0000-0002-5777-3464"},{"name":"Narehood SM","author_orcid":"0000-0001-6822-8608"},{"name":"McGuire KL","author_orcid":"0009-0005-7520-3675"},{"name":"Li Y","author_orcid":"0009-0000-5502-4243"},{"name":"Akif Tezcan F","author_orcid":"0000-0002-4733-6500"},{"name":"Herzik Jr MA","author_orcid":"0000-0001-6653-6682"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Preparation of oxygen-sensitive proteins for high-resolution cryoEM structure determination using blot-free vitrification","volume":"16","country":"","first_page":"3528","last_page":"3528","year":"2025","language":"English","doi":"10.1038/s41467-025-58243-1","pubmedid":"40229244","details":"CryoEM movies of human hemoglobin (Hb) in the presence of 5 mM sodium dithionite under Al's oil. Data were collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2 with a defocus range of − 1 to − 2.5 µm. Data were collected automatically using EPU with aberration free image shift.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature communications","journal_abbreviation":"Nat Commun","issue":"1","preprint":false}],"dataset_size":"1.2 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9cqs","9cqt"],"entry_doi":"10.6019/EMPIAR-12744"}}