{"EMPIAR-12723":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of FA-CP inhibited ribosomes","directory":"data/FA-CP_Krios_dataset/raw_movies","category":"micrographs - multiframe","header_format":"EER","data_format":"EER","num_images_or_tilt_series":17252,"frames_per_image":684,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.728,"pixel_height":0.728,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Data (raw movies) were collected at SciLifeLab in Umeå, Sweden in a Titan Krios G2 operated at 300 kV and equipped with a Falcon-4i direct electron detector and Selectris energy filter using 10 eV slit. The data were acquired at 165,000 x nominal magnification with a calibrated pixel size of 0.728 Å. A 50 µm C2 aperture was inserted and the TEM was operated in nanoprobe mode at spot size 6 and 520 nm beam size. The EER formatted movies were collected in 684 raw frames with a total dose of 27.77 e-/Å2 (12.45 e-/pixel/s) over 2.23 s with a set defocus between -0.7 to -1.3 µm.\nThe movies are not gain-corrected, use the file provided within the main folder.","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of FA inhibited ribosomes","directory":"data/FA_Krios_dataset/raw_movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":5486,"frames_per_image":30,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.824,"pixel_height":0.824,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Data (raw movies) were collected at SciLifeLab in Solna, Sweden on a Titan Krios G2 operated at 300 kV and equipped with a K3 BioQuantum direct electron detector and energy filter using 20 eV slit. The data were acquired at 105,000 x nominal magnification with a calibrated pixel size of 0.824 Å. A 50 µm C2 aperture was inserted and the TEM was operated in nanoprobe mode at spot size 6 and 670 nm beam size. The movies were collected in 30 frames over 1.3 s with a total dose of 30.83 e-/Å2 (16.1 e-/pixel/s) with a set defocus between -0.5 to -1.5 µm.\nThe movies are gain-corrected.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"EMD-17364 particles (FA-CP CHI)","directory":"data/FA-CP_Krios_dataset/particles_EMD-17364_PDB-8P2G","category":"picked particles - single frame - processed","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":303005,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.728,"pixel_height":0.728,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"","image_width":"700","image_height":"700"},{"segmentations":[],"name":"EMD-17363 particles (FA-CP POST)","directory":"data/FA-CP_Krios_dataset/particles_EMD-17363_PDB-8P2F","category":"picked particles - single frame - processed","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":40800,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.728,"pixel_height":0.728,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"","image_width":"700","image_height":"700"},{"segmentations":[],"name":"EMD-17365 particles (FA CHI)","directory":"data/FA_Krios_dataset/particles_EMD-17365_PDB-8P2H","category":"picked particles - single frame - processed","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":178679,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.824,"pixel_height":0.824,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"","image_width":"600","image_height":"600"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of FA-CP inhibited ribosomes screening Glacios data","directory":"data/FA-CP_Glacios_screening_data/raw_movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":6052,"frames_per_image":31,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":0.952,"pixel_height":0.952,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Raw movies from the FA-CP inhibited ribosomes screening in a Glacios microscope operated at 200 kV with a magnification of 150,000x with a pixel size of 0.952 Å/pix. The movies were collected in 31 frames over 28.74 s with a total dose of 29.8 e-/Å2 (0.94 e-/pixel/s) with a set defocus between -0.5 to -1.5 µm.\nThe movies are gain corrected.","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of FA inhibited ribosomes screening Glacios data","directory":"data/FA_Glacios_screening_data/raw_movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":1460,"frames_per_image":30,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":0.952,"pixel_height":0.952,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Raw movies from the FA inhibited ribosomes screening in a Glacios microscope operated at 200 kV with a magnification of 150,000x with a pixel size of 0.952 Å/pix. The movies were collected in 30 frames over 27.79 s with a total dose of 30 e-/Å2 (1.00 e-/pixel/s) with a set defocus between -0.5 to -1.5 µm.\nThe movies are gain corrected.","image_width":"4096","image_height":"4096"}],"workflow_file":null,"grant_references":[{"funding_body":"Swedish Research Council","code":"2017-03827","country":"Sweden"},{"funding_body":"Swedish Research Council","code":"2022-04511","country":"Sweden"}],"version_history":[],"title":"Single-particle cryo-EM of Staphylococcus aureus ribosomes inhibited by fusidic acid and fusidic acid cyclopentane","principal_investigator":[{"author_orcid":"0000-0001-9079-2774","middle_name":null,"organization":"Department of Cell and Molecular Biology (ICM), Uppsala University","street":"Box 596","town_or_city":"Uppsala","state_or_province":"Uppland","post_or_zip":"75124","telephone":null,"fax":null,"first_name":"Maria","last_name":"Selmer","email":"maria.selmer [at] icm.uu.se","country":"Sweden","entry":"EMPIAR-12723"}],"status":"REL","deposition_date":"2025-04-04","release_date":"2025-05-14","obsolete_date":null,"update_date":"2025-05-14","corresponding_author":{"author":{"author_orcid":"0000-0002-4302-6855","middle_name":null,"organization":"Department of Cell and Molecular Biology (ICM), Uppsala University","street":"Box 596","town_or_city":"Uppsala","state_or_province":"Uppland","post_or_zip":"75124","first_name":"Adrián","last_name":"González-López","country":"Sweden"}},"authors":[{"author":{"name":"González-López A","author_orcid":"0000-0002-4302-6855"}},{"author":{"name":"Larsson DSD","author_orcid":"0000-0001-7683-6419"}},{"author":{"name":"Selmer M","author_orcid":"0000-0001-9079-2774"}}],"cross_references":["EMD-17365","EMD-17363","EMD-17364"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"González-López A","author_orcid":"0000-0002-4302-6855"},{"name":"Larsson DSD","author_orcid":null},{"name":"Koripella RK","author_orcid":null},{"name":"Cain BN","author_orcid":null},{"name":"Chavez MG","author_orcid":null},{"name":"Hergenrother PJ","author_orcid":null},{"name":"Sanyal S","author_orcid":null},{"name":"Selmer M","author_orcid":"0000-0001-9079-2774"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structures of the Staphylococcus aureus ribosome inhibited by fusidic acid and fusidic acid cyclopentane","volume":"14","country":"","first_page":null,"last_page":null,"year":"2024","language":"English","doi":"10.1038/s41598-024-64868-x","pubmedid":"38902339","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Scientific reports","journal_abbreviation":"Sci Rep","issue":"1","preprint":false}],"dataset_size":"11.1 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["8p2h","8p2f","8p2g"],"entry_doi":"10.6019/EMPIAR-12723"}}