{"EMPIAR-12705":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame movies of the TIGR-TasR RNP bound to target DNA","directory":"data/micrographs","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":14257,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.663,"pixel_height":0.663,"micrographs_file_pattern":"data/micrographs/GridSquare_*/Data/FoilHole_*_Data_*_fractions.tiff","picked_particles_file_pattern":"data/EMD-48616_refine_data.star","picked_particles_directory":"","details":"Unaligned, non-gain-normalised, 40-frame movies from a Bioquantum-K3 camera recorded in super-resolution mode with twofold binning (within EPU) at 130,000x magnification on the MIT.nano Titan Krios G3i microscope operated at 300 keV (Cs = 2.7mm). Each micrograph accumulates 45.4 e/A2 over a 1.09s exposure time. The final refinement run_data.star file for the EMDB deposition is included as 'EMD-46818_refine_data.star', although you will need to edit the micrograph path within this file if you wish to reuse the picked coordinates. Good luck! This dataset is a little bit tricky.","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo-EM micrographs of TIGR-TasR in complex with tigRNA and target DNA after DNA cleavage","principal_investigator":[{"author_orcid":"0000-0003-0178-7995","middle_name":null,"organization":"Broad Institute of Harvard and MIT","street":"75 Ames Street","town_or_city":"Cambridge","state_or_province":"Massachusetts","post_or_zip":"02142","telephone":"6177147000","fax":null,"first_name":"Feng","last_name":"Zhang","email":"zhang [at] broadinstitute.org","country":"United States","entry":"EMPIAR-12705"}],"status":"REL","deposition_date":"2025-04-08","release_date":"2025-04-23","obsolete_date":null,"update_date":"2025-04-23","corresponding_author":{"author":{"author_orcid":"0000-0003-4738-9503","middle_name":null,"organization":"Massachusetts Institute of Technology","street":"75 Ames Street","town_or_city":"Cambridge","state_or_province":"Massachusetts","post_or_zip":"02142","first_name":"Max","last_name":"Wilkinson","country":"United States"}},"authors":[{"author":{"name":"Faure G","author_orcid":"0000-0001-9537-2277"}},{"author":{"name":"Saito M","author_orcid":"0000-0001-8619-5176"}},{"author":{"name":"Wilkinson ME","author_orcid":"0000-0003-4738-9503"}},{"author":{"name":"Quinones-Olvera N","author_orcid":"0000-0002-4612-6819"}},{"author":{"name":"Xu P","author_orcid":"0000-0003-3590-4037"}},{"author":{"name":"Flam-Shepherd D","author_orcid":"0000-0002-9568-3451"}},{"author":{"name":"Kim S","author_orcid":"0000-0002-5116-0798"}},{"author":{"name":"Reddy N","author_orcid":"0000-0001-5912-6925"}},{"author":{"name":"Zhu S","author_orcid":"0009-0001-2677-8262"}},{"author":{"name":"Evgeniou L","author_orcid":"0009-0005-5707-2413"}},{"author":{"name":"Koonin EV","author_orcid":"0000-0003-3943-8299"}},{"author":{"name":"Macrae RK","author_orcid":"0000-0002-7850-9056"}},{"author":{"name":"Zhang F","author_orcid":"0000-0003-0178-7995"}}],"cross_references":["EMD-48616"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Faure G","author_orcid":"0000-0001-9537-2277"},{"name":"Saito M","author_orcid":"0000-0001-8619-5176"},{"name":"Wilkinson ME","author_orcid":"0000-0003-4738-9503"},{"name":"Quinones-Olvera N","author_orcid":"0000-0002-4612-6819"},{"name":"Xu P","author_orcid":"0000-0003-3590-4037"},{"name":"Flam-Shepherd D","author_orcid":"0000-0002-9568-3451"},{"name":"Kim S","author_orcid":"0000-0002-5116-0798"},{"name":"Reddy N","author_orcid":"0000-0001-5912-6925"},{"name":"Zhu S","author_orcid":"0009-0001-2677-8262"},{"name":"Evgeniou L","author_orcid":"0009-0005-5707-2413"},{"name":"Koonin EV","author_orcid":"0000-0003-3943-8299"},{"name":"Macrae RK","author_orcid":"0000-0002-7850-9056"},{"name":"Zhang F","author_orcid":"0000-0003-0178-7995"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"TIGR-Tas: A family of modular RNA-guided DNA-targeting systems in prokaryotes and their viruses","volume":null,"country":"United States","first_page":null,"last_page":null,"year":"2025","language":"English","doi":"10.1126/science.adv9789","pubmedid":"40014690","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Science (New York, N.Y.)","journal_abbreviation":"Science","issue":null,"preprint":false}],"dataset_size":"1.9 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9mty"],"entry_doi":"10.6019/EMPIAR-12705"}}