{"EMPIAR-12613":{"imagesets":[{"segmentations":[],"name":"Unaligned multiframe micrographs of Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site 2'-deoxy-A76-fMEAAAKC-peptidyl-tRNAcys","directory":"data/EMPIAR_upload","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":25104,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.8423,"pixel_height":0.8423,"micrographs_file_pattern":"data/EMPIAR_upload/*.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Gain reference image for uploaded unaligned micrographs","directory":"data/gain-ref","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.8423,"pixel_height":0.8423,"micrographs_file_pattern":"data/gain-ref/CountRef_2024-11-26_18.18.24_112624_Tth70S-RF1-2dO_65-12_X+0Y+0-1.mrc","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Gain reference file for the accompanying raw micrographs","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo-EM structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site 2'-deoxy-A76-fMEAAAKC-peptidyl-tRNAcys","principal_investigator":[{"author_orcid":"0000-0003-4516-604X","middle_name":"G","organization":"Department of Microbiology and Immunology, University of Texas Medical Branch","street":"301 University Blvd","town_or_city":"Galveston","state_or_province":"Texas","post_or_zip":"77555","telephone":"+1 409 772 2326","fax":null,"first_name":"Matthieu","last_name":"Gagnon","email":"magagnon [at] utmb.edu","country":"United States","entry":"EMPIAR-12613"}],"status":"REL","deposition_date":"2025-02-28","release_date":"2025-07-12","obsolete_date":null,"update_date":"2025-07-12","corresponding_author":{"author":{"author_orcid":"0000-0001-6410-7905","middle_name":null,"organization":"Department of Microbiology and Immunology, University of Texas Medical Branch","street":"301 University Blvd","town_or_city":"Galveston","state_or_province":"Texas","post_or_zip":"77555","first_name":"Ritwika","last_name":"Basu","country":"United States"}},"authors":[{"author":{"name":"Basu RS","author_orcid":"0000-0001-6410-7905"}},{"author":{"name":"Gagnon MG","author_orcid":"0000-0003-4516-604X"}},{"author":{"name":"Polikanov YS","author_orcid":"0000-0002-5064-0327"}}],"cross_references":["EMD-49594"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Aleksandrova EV","author_orcid":"0000-0002-1808-5319"},{"name":"Syroegin EA","author_orcid":"0000-0003-2753-3950"},{"name":"Basu RS","author_orcid":"0000-0001-6410-7905"},{"name":"Vassilevski AA","author_orcid":"0000-0002-1359-0076"},{"name":"Gagnon MG","author_orcid":"0000-0003-4516-604X"},{"name":"Polikanov YS","author_orcid":"0000-0002-5064-0327"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Mechanism of release factor-mediated peptidyl-tRNA hydrolysis on the ribosome","volume":"388","country":"United States","first_page":null,"last_page":null,"year":"2025","language":"English","doi":"10.1126/science.ads9030","pubmedid":"40536958","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Science (New York, N.Y.)","journal_abbreviation":"Science","issue":"6753","preprint":false}],"dataset_size":"4.1 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9no7"],"entry_doi":"10.6019/EMPIAR-12613"}}