{"EMPIAR-12557":{"imagesets":[{"segmentations":[],"name":"Unaligned mutli-frame FIB/SEM micrograph of the mice molecular layer of the hippocampus.","directory":"data/ESB_Stack_1","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":2501,"frames_per_image":1,"frame_range_min":1,"frame_range_max":3301,"voxel_type":"UNSIGNED BYTE","pixel_width":40.0,"pixel_height":40.0,"micrographs_file_pattern":"data/ESB_Stack_1/slice_00001_z=0.0325um.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned FIB-SEM dataset of the mouse dentate gyrus of the hippocampus acquired at 4 nm isotropic voxel size, deposited as a single-frame image sequence in TIFF format. Details of the imaging procedure and exploitation of the dataset are available in the original publication","image_width":"3266","image_height":"2761"},{"segmentations":[],"name":"Unaligned mutli-frame FIB/SEM micrograph of the mice molecular layer of the hippocampus.","directory":"data/ESB_Stack_2","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":3058,"frames_per_image":1,"frame_range_min":1,"frame_range_max":3058,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":40.0,"pixel_height":40.0,"micrographs_file_pattern":"data/ESB_Stack_2/slice_00001_z=0.0175um.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned FIB-SEM dataset of the mouse dentate gyrus of the hippocampus acquired at 4 nm isotropic voxel size, deposited as a single-frame image sequence in TIFF format. Details of the imaging procedure and exploitation of the dataset are available in the original publication","image_width":"8768","image_height":"8768"},{"segmentations":[],"name":"Unaligned mutli-frame FIB/SEM micrograph of the mice molecular layer of the hippocampus.","directory":"data/ESB_Stack_3","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":1108,"frames_per_image":1,"frame_range_min":1,"frame_range_max":1108,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":40.0,"pixel_height":40.0,"micrographs_file_pattern":"data/ESB_Stack_3/slice_00001_z=0.0196um.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned FIB-SEM dataset of the mouse dentate gyrus of the hippocampus acquired at 4 nm isotropic voxel size, deposited as a single-frame image sequence in TIFF format. Details of the imaging procedure and exploitation of the dataset are available in the original publication","image_width":"7488","image_height":"7488"},{"segmentations":[],"name":"Unaligned multi-frame FIB/SEM micrograph of the mouse molecular layer of the hippocampus after immunogold pre-embedding for IP3Rs.","directory":"data/ESB_IP3Rs_Immunogold","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":3301,"frames_per_image":1,"frame_range_min":1,"frame_range_max":3301,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":40.0,"pixel_height":40.0,"micrographs_file_pattern":"data/ESB_IP3Rs_Immunogold/slice_00001_z=0.0607um.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned FIB-SEM dataset of the mouse dentate gyrus of the hippocampus with immunogold staining acquired at 4 nm isotropic voxel size, deposited as a single-frame image sequence in TIFF format. Details of the imaging procedure and exploitation of the dataset are available in the original publication","image_width":"5504","image_height":"5504"},{"segmentations":[],"name":"Aligned multi-frame (as a multipage tif) FIB/SEM micrograph of the mouse molecular layer of the hippocampus after immunogold pre-embedding for Cx43","directory":"data/ESB_Cx43_Immunogold","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":2395,"frames_per_image":1,"frame_range_min":1,"frame_range_max":2395,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":40.0,"pixel_height":40.0,"micrographs_file_pattern":"data/ESB_Cx43_Immunogold/Stack_750_04_140_2395_TM1b (4 nm).tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Aligned and cropped FIB-SEM dataset of the mouse dentate gyrus of the hippocampus with immunogold staining acquired at 4 nm isotropic voxel size, deposited as a multipage TIFF . Details of the imaging procedure and exploitation of the dataset are available in the original publication","image_width":"3599","image_height":"4342"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"3D FIB/SEM Imaging of the Molecular Layer of the Mouse Dentate Gyrus","principal_investigator":[{"author_orcid":"0000-0003-1824-7899","middle_name":null,"organization":"Univ. Grenoble Alpes, Inserm, U1216, Grenoble Institut Neurosciences, F-38000 Grenoble, France","street":null,"town_or_city":"Grenoble","state_or_province":null,"post_or_zip":"38700","telephone":null,"fax":null,"first_name":"Lucas","last_name":"Benoit","email":"lucasbentpro [at] gmail.com","country":"France","entry":"EMPIAR-12557"},{"author_orcid":"0000-0003-3468-3316","middle_name":null,"organization":"Univ. Grenoble Alpes, Inserm, U1216, Grenoble Institut Neurosciences, F-38000 Grenoble, France","street":null,"town_or_city":"Grenoble","state_or_province":null,"post_or_zip":"38700","telephone":null,"fax":null,"first_name":"Karin","last_name":"Pernet-Gallay","email":"karin.pernet-gallay [at] univ-grenoble-alpes.fr","country":"France","entry":"EMPIAR-12557"},{"author_orcid":"0000-0003-1069-1602","middle_name":null,"organization":"Department of Fundamental Neurosciences, University of Lausanne, Rue du Bugnon 9, 1005 Lausanne, Switzerland","street":null,"town_or_city":"Lausanne","state_or_province":null,"post_or_zip":"1005","telephone":null,"fax":null,"first_name":"Andrea","last_name":"Volterra","email":"andrea.volterra [at] unil.ch","country":"Switzerland","entry":"EMPIAR-12557"}],"status":"REL","deposition_date":"2025-01-24","release_date":"2025-10-23","obsolete_date":null,"update_date":"2025-10-23","corresponding_author":{"author":{"author_orcid":"0000-0003-3468-3316","middle_name":null,"organization":"Univ. Grenoble Alpes, Inserm, U1216, Grenoble Institut Neurosciences, F-38000 Grenoble, France","street":null,"town_or_city":"Grenoble","state_or_province":null,"post_or_zip":"38700","first_name":"Karin","last_name":"Pernet-Gallay","country":"France"}},"authors":[{"author":{"name":"Benoit LB","author_orcid":"0000-0003-1824-7899"}},{"author":{"name":"Pernet-Gallay KPG","author_orcid":"0000-0003-3468-3316"}},{"author":{"name":"Jouneau PHJ","author_orcid":"0000-0003-0350-4901"}}],"cross_references":[],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Benoit LB","author_orcid":"0000-0003-1824-7899"},{"name":"Hristovska IH","author_orcid":null},{"name":"Liaudet NL","author_orcid":"0000-0002-0749-5857"},{"name":"De-Ceglia RDC","author_orcid":"0000-0001-6264-677X"},{"name":"Jouneau PHJ","author_orcid":"0000-0003-0350-4901"},{"name":"Fertin AF","author_orcid":"0000-0001-9563-9103"},{"name":"Litvin DL","author_orcid":"0000-0001-7688-5894"},{"name":"Telley LT","author_orcid":"0000-0002-5041-1997"},{"name":"Jevtic JM","author_orcid":null},{"name":"Bergami MB","author_orcid":null},{"name":"Usson YU","author_orcid":null},{"name":"Hisatsune CH","author_orcid":null},{"name":"Mikoshiba KM","author_orcid":null},{"name":"Pernet-Gallay KPG","author_orcid":"0000-0003-3468-3316"},{"name":"Volterra AV","author_orcid":"0000-0003-1069-1602"}],"editors":[],"published":false,"j_or_nj_citation":true,"title":"Astrocytes functionally integrate single or multiple synapses via the specialized leaflet compartment","volume":null,"country":"","first_page":null,"last_page":null,"year":null,"language":"English","doi":null,"pubmedid":null,"details":"This archive regroups the different raw FIB/SEM stacks used in the publication. Stacks 1, 2, 3, and IP3Rs immunogold are not aligned and are furnishe as a tif sequence folder, whereas Cx43 stacks have been aligned and is furnished as a mutlipage tif. The stack of 2 images was acquired under conditions with an isotropic voxel size of 4 nm.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"","journal_abbreviation":"","issue":null,"preprint":false}],"dataset_size":"258.2 GB","experiment_type":"FIB-SEM","scale":"tissue","entry_doi":"10.6019/EMPIAR-12557"}}