{"EMPIAR-12549":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of Cholinephosphotransferase in complex with CDP-choline and phosphatidylcholine (dataset 1)","directory":"data/20230215_frames","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":3566,"frames_per_image":60,"frame_range_min":1,"frame_range_max":60,"voxel_type":"UNSIGNED BYTE","pixel_width":1.09,"pixel_height":1.09,"micrographs_file_pattern":"data/20230215_frames/*.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Micrographs are unaligned 60 raw movie frames accumulating a total dose of 63 electrons per square angstrom in a 3.47 second exposure.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of Cholinephosphotransferase in complex with CDP-choline and phosphatidylcholine (dataset 2)","directory":"data/20230222_frames","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":2563,"frames_per_image":60,"frame_range_min":1,"frame_range_max":60,"voxel_type":"UNSIGNED BYTE","pixel_width":1.08,"pixel_height":1.08,"micrographs_file_pattern":"data/20230222_frames/*.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Micrographs are unaligned 60 raw movie frames accumulating a total dose of 63 electrons per square angstrom in a 3.47 second exposure.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of Cholinephosphotransferase in complex with CDP-choline and phosphatidylcholine (dataset 3)","directory":"data/20230301_frames","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":2943,"frames_per_image":60,"frame_range_min":1,"frame_range_max":60,"voxel_type":"UNSIGNED BYTE","pixel_width":1.08,"pixel_height":1.08,"micrographs_file_pattern":"data/20230301_frames/*.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Micrographs are unaligned 60 raw movie frames accumulating a total dose of 57 electrons per square angstrom in a 3.47 second exposure.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of Cholinephosphotransferase in complex with CDP-choline and phosphatidylcholine with 3.25 mM CHAPS added before plunging (dataset 4)","directory":"data/20230302_CHAPS_frames","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":2522,"frames_per_image":60,"frame_range_min":1,"frame_range_max":60,"voxel_type":"UNSIGNED BYTE","pixel_width":1.08,"pixel_height":1.08,"micrographs_file_pattern":"data/20230302_CHAPS_frames/*.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"3.25 mM CHAPS added before plunging to ameliorate preferred orientation. Micrographs are unaligned 60 raw movie frames accumulating a total dose of 61 electrons per square angstrom in a 3.79 second exposure.","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health (NIH)","code":"S10OD030275","country":"United States"}],"version_history":[],"title":"Cryo-electron micrographs of Cholinephosphotransferase in complex with CDP-choline and phosphatidylcholine","principal_investigator":[{"author_orcid":"0000-0003-1750-4793","middle_name":"D","organization":"Life Sciences Institute, University of Michigan","street":"210 Washtenaw Ave","town_or_city":"Ann Arbor","state_or_province":"Michigan","post_or_zip":"48109","telephone":null,"fax":null,"first_name":"Melanie","last_name":"Ohi","email":"mohi [at] umich.edu","country":"United States","entry":"EMPIAR-12549"},{"author_orcid":"0000-0002-7762-9793","middle_name":null,"organization":"Department of Pharmacology, University of Michigan School of Medicine","street":"1150 W Medical Center Dr","town_or_city":"Ann Arbor","state_or_province":"Michigan","post_or_zip":"48109","telephone":null,"fax":null,"first_name":"Shoji","last_name":"Maeda","email":"maesyou [at] gmail.com","country":"United States","entry":"EMPIAR-12549"}],"status":"REL","deposition_date":"2025-02-10","release_date":"2025-07-25","obsolete_date":null,"update_date":"2025-07-25","corresponding_author":{"author":{"author_orcid":"0000-0001-9976-5828","middle_name":"Renee","organization":"Department of Biological Chemistry, University of Michigan","street":"1136 Catherine St","town_or_city":"Ann Arbor","state_or_province":"Michigan","post_or_zip":"48109","first_name":"Jacquelyn","last_name":"Roberts","country":"United States"}},"authors":[{"author":{"name":"Roberts JR","author_orcid":"0000-0001-9976-5828"}}],"cross_references":["EMD-42496"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Roberts JR","author_orcid":"0000-0001-9976-5828"},{"name":"Horibata Y","author_orcid":"0009-0004-3384-4354"},{"name":"Kwarcinski FE","author_orcid":null},{"name":"Lam V","author_orcid":"0000-0001-8228-2133"},{"name":"Raczkowski AM","author_orcid":null},{"name":"Hubbard A","author_orcid":"0009-0005-5855-6022"},{"name":"White B","author_orcid":null},{"name":"Sugimoto H","author_orcid":null},{"name":"Tall GG","author_orcid":"0000-0002-6540-4896"},{"name":"Ohi MD","author_orcid":"0000-0003-1750-4793"},{"name":"Maeda S","author_orcid":"0000-0002-7762-9793"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structural basis for catalysis and selectivity of phospholipid synthesis by eukaryotic choline-phosphotransferase","volume":"16","country":"","first_page":null,"last_page":null,"year":"2025","language":"English","doi":"10.1038/s41467-024-55673-1","pubmedid":"39747155","details":"CryoEM datasets of yeast cholinephosphotransferase in complex with CDP choline and phosphatidylcholine. A total of 11,596 multi-frame micrographs (each consisting of 60 frames in TIF format) were acquired. This includes three datasets without CHAPS, comprising 9,074 micrographs, and one dataset with 2,522 micrographs collected in the presence of 3.25 mM CHAPS to mitigate preferred orientation. [11,596 total  multi-frame micrographs composed of 60 frames each in TIF format].","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature communications","journal_abbreviation":"Nat Commun","issue":"1","preprint":false}],"dataset_size":"4.0 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["8urp"],"entry_doi":"10.6019/EMPIAR-12549"}}