{"EMPIAR-12476":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame data of AsCas12f-sgRNA-DNA complex","directory":"data","category":"micrographs - multiframe","header_format":"MRC","data_format":"TIFF","num_images_or_tilt_series":7591,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.5325,"pixel_height":0.5325,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Total dose: 50 electrons per square angstrom. Gain image included as a mrc file.","image_width":"11520","image_height":"8184"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)","code":"R35GM143052","country":"United States"}],"version_history":[],"title":"single-particle cryoEM data of AsCas12f-sgRNA-DNA complex","principal_investigator":[{"author_orcid":"0000-0001-5832-6060","middle_name":null,"organization":"Department of Biochemistry and Molecular Biology, The University of Chicago","street":"929 E 57th Street, W201","town_or_city":"Chicago","state_or_province":"IL","post_or_zip":"60637","telephone":null,"fax":null,"first_name":"Minglei","last_name":"Zhao","email":"mlzhao [at] uchicago.edu","country":"United States","entry":"EMPIAR-12476"}],"status":"REL","deposition_date":"2024-12-03","release_date":"2025-02-04","obsolete_date":null,"update_date":"2025-02-04","corresponding_author":{"author":{"author_orcid":"0000-0001-5832-6060","middle_name":null,"organization":"Department of Biochemistry and Molecular Biology, The University of Chicago","street":"929 E 57th Street, W201","town_or_city":"Chicago","state_or_province":"IL","post_or_zip":"60637","first_name":"Minglei","last_name":"Zhao","country":"United States"}},"authors":[{"author":{"name":"Zhao M","author_orcid":"0000-0001-5832-6060"}},{"author":{"name":"Liu C","author_orcid":"0000-0001-9373-8458"}}],"cross_references":["EMD-27801"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Wu T","author_orcid":"0000-0001-9968-9909"},{"name":"Liu C","author_orcid":"0000-0001-9373-8458"},{"name":"Zou S","author_orcid":"0009-0005-7771-3644"},{"name":"Lyu R","author_orcid":null},{"name":"Yang B","author_orcid":null},{"name":"Yan H","author_orcid":null},{"name":"Zhao M","author_orcid":"0000-0001-5832-6060"},{"name":"Tang W","author_orcid":"0000-0002-5739-5416"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"An engineered hypercompact CRISPR-Cas12f system with boosted gene-editing activity","volume":"19","country":"","first_page":"1384","last_page":"1393","year":"2023","language":"English","doi":"10.1038/s41589-023-01380-9","pubmedid":"37400536","details":"Raw micrographs collected at the Advanced Electron Microscopey Center at the University of Chicago using a Titan Krios equipped with a K3 detector","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature chemical biology","journal_abbreviation":"Nat Chem Biol","issue":"11","preprint":false}],"dataset_size":"3.9 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["8dzj"],"entry_doi":"10.6019/EMPIAR-12476"}}