{"EMPIAR-12444":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame movies of E. coli TnsB in complex with right end fragment of Tn7 transposon","directory":"data/Movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":6692,"frames_per_image":40,"frame_range_min":1,"frame_range_max":40,"voxel_type":"UNSIGNED BYTE","pixel_width":0.86,"pixel_height":0.86,"micrographs_file_pattern":"data/Movies/*.tiff","picked_particles_file_pattern":"data/TnsB_EMD-13440/EMD-13440_particlestack.star","picked_particles_directory":"data/TnsB_EMD-13440","details":"Gain-corrected, unaligned multi-frame movies collected with Krios G3i microscope + K3 detector (superresolution mode, binned 2x with EPU). Voltage: 300 kV; spherical aberration: 2.7; dose per frame: 1 e/A^2 (total dose: 40.16 e/A^2 fractionated over 40 frames). Defocus range (nominal): -2.0, -1.7, -1.4, -1.1, -0.8, -0.5 µm. Objective aperture retracted, C2 aperture: 50 µm.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Aligned particles of E. coli TnsB in complex with right end fragment of Tn7 transposon (helical reconstruction)","directory":"data/TnsB_EMD-13440","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":450748,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.86,"pixel_height":0.86,"micrographs_file_pattern":"data/Movies/*.tiff","picked_particles_file_pattern":"data/TnsB_EMD-13440/EMD-13440_particlestack.star","picked_particles_directory":"data/TnsB_EMD-13440","details":"Aligned particles with corresponding Relion-3 STAR file for helical reconstruction. Coordinates correspond to micrographs binned 2x during Motion correction.","image_width":"512","image_height":"512"},{"segmentations":[],"name":"Aligned particles of E. coli TnsB in complex with right end fragment of Tn7 transposon (single particle reconstruction)","directory":"data/TnsB_EMD-13439","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":901496,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.86,"pixel_height":0.86,"micrographs_file_pattern":"data/Movies/*.tiff","picked_particles_file_pattern":"data/TnsB_EMD-13439/EMD-13439_particlestack.star","picked_particles_directory":"data/TnsB_EMD-13439","details":"Aligned particles with corresponding Relion-3 STAR file for single particle reconstruction. Particles were signal-subtracted; coordinates correspond to micrographs binned 2x during Motion correction.","image_width":"512","image_height":"512"}],"workflow_file":null,"grant_references":[{"funding_body":"Foundation for Polish Science","code":"POIR.04.04.00-00-20E7/16-00","country":"Poland"},{"funding_body":"National Institutes of Health (NIH)","code":"R01GM129118","country":"United States"},{"funding_body":"European Union (EU)","code":"POIG.02.02.00-14-024/08-00","country":"Poland"},{"funding_body":"Other government","code":"1/SOL/2021/2","country":"Poland"}],"version_history":[],"title":"Single particle reconstruction of E. coli TnsB in complex with right end fragment of Tn7 transposon","principal_investigator":[{"author_orcid":"0000-0001-8632-0977","middle_name":null,"organization":"International Institute of Molecular and Cell Biology in Warsaw","street":"4 Ks. Trojdena Street","town_or_city":"Warsaw","state_or_province":null,"post_or_zip":"02-109","telephone":null,"fax":null,"first_name":"Marcin","last_name":"Nowotny","email":"mnowotny [at] iimcb.gov.pl","country":"Poland","entry":"EMPIAR-12444"}],"status":"REL","deposition_date":"2024-11-02","release_date":"2025-02-03","obsolete_date":null,"update_date":"2025-02-27","corresponding_author":{"author":{"author_orcid":"0000-0001-5582-2851","middle_name":null,"organization":"International Institute of Molecular and Cell Biology in Warsaw","street":"4 Ks. Trojdena Street","town_or_city":"Warsaw","state_or_province":null,"post_or_zip":"02-109","first_name":"Mariusz","last_name":"Czarnocki-Cieciura","country":"Poland"}},"authors":[{"author":{"name":"Kaczmarska Z","author_orcid":"0000-0002-7668-1442"}},{"author":{"name":"Czarnocki-Cieciura M","author_orcid":"0000-0001-5582-2851"}},{"author":{"name":"Rawski M","author_orcid":"0000-0002-8553-7637"}},{"author":{"name":"Nowotny M","author_orcid":"0000-0001-8632-0977"}}],"cross_references":["EMD-13439","EMD-13440"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Kaczmarska Z","author_orcid":"0000-0002-7668-1442"},{"name":"Czarnocki-Cieciura M","author_orcid":"0000-0001-5582-2851"},{"name":"Górecka-Minakowska KM","author_orcid":"0000-0002-9843-3888"},{"name":"Wingo RJ","author_orcid":"0000-0002-0831-3234"},{"name":"Jackiewicz J","author_orcid":"0000-0003-1790-1967"},{"name":"Zajko W","author_orcid":"0000-0001-9915-0305"},{"name":"Poznański JT","author_orcid":"0000-0003-2684-1775"},{"name":"Rawski M","author_orcid":"0000-0002-8553-7637"},{"name":"Grant T","author_orcid":"0000-0002-4855-8703"},{"name":"Peters JE","author_orcid":"0000-0001-8309-3297"},{"name":"Nowotny M","author_orcid":"0000-0001-8632-0977"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structural basis of transposon end recognition explains central features of Tn7 transposition systems","volume":"82","country":"","first_page":"2618","last_page":"2632.e7","year":"2022","language":"English","doi":"10.1016/j.molcel.2022.05.005","pubmedid":"35654042","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Molecular cell","journal_abbreviation":"Mol Cell","issue":"14","preprint":false}],"dataset_size":"3.7 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["7pik"],"entry_doi":"10.6019/EMPIAR-12444"}}