{"EMPIAR-12443":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame movies of human apo TRPM8 channel in a closed state","directory":"data/Movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":7918,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.82,"pixel_height":0.82,"micrographs_file_pattern":"data/Movies/*.tiff","picked_particles_file_pattern":"data/TRPM8_EMD-15982/EMD-15982_particlestack.star","picked_particles_directory":"data/TRPM8_EMD-15982","details":"Gain-corrected, unaligned multi-frame movies collected with Krios G3i microscope + K3 detector (superresolution mode, binned 2x with EPU). Voltage: 300 kV; spherical aberration: 2.7; dose per frame: 1 e/A^2 (total dose: 41.42 e/A^2 fractionated over 40 frames). Defocus range (nominal): -2.7, -2.4, -2.1, -1.8, -1.5, -1.2, -0.9 µm. Objective aperture retracted, C2 aperture: 50 µm.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Aligned particles of human apo TRPM8 channel in a closed state (consensus map)","directory":"data/TRPM8_EMD-15982","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":110176,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.82,"pixel_height":0.82,"micrographs_file_pattern":"data/Movies/*.tiff","picked_particles_file_pattern":"data/TRPM8_EMD-15982/EMD-15982_particlestack.star","picked_particles_directory":"data/TRPM8_EMD-15982","details":"Aligned particles with corresponding Relion-3 STAR file for the consensus TRPM8 reconstruction. Coordinates correspond to micrographs binned 2x during Motion correction.","image_width":"420","image_height":"420"},{"segmentations":[],"name":"Aligned particles of human apo TRPM8 channel in a closed state (focused pre-MHR + MHR1/2 map)","directory":"data/TRPM8_EMD-15983","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":212851,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.82,"pixel_height":0.82,"micrographs_file_pattern":"data/Movies/*.tiff","picked_particles_file_pattern":"data/TRPM8_EMD-15983/EMD-15983_particlestack.star","picked_particles_directory":"data/TRPM8_EMD-15983","details":"Aligned particles with corresponding Relion-3 STAR file for the focused TRPM8 reconstruction (pre-MHR + MHR1/2 map). Particles were signal-subtracted; coordinates correspond to micrographs binned 2x during Motion correction.","image_width":"192","image_height":"192"}],"workflow_file":null,"grant_references":[{"funding_body":"Other government","code":"1/SOL/2021/2","country":"Poland"},{"funding_body":"Other government","code":"F/090033/01-03-04/X36","country":"Italy"}],"version_history":[],"title":"Single particle reconstruction of human apo TRPM8 channel in a closed state","principal_investigator":[{"author_orcid":"0000-0001-8632-0977","middle_name":null,"organization":"International Institute of Molecular and Cell Biology in Warsaw","street":"4 Ks. Trojdena Street","town_or_city":"Warsaw","state_or_province":null,"post_or_zip":"02-109","telephone":null,"fax":null,"first_name":"Marcin","last_name":"Nowotny","email":"mnowotny [at] iimcb.gov.pl","country":"Poland","entry":"EMPIAR-12443"}],"status":"REL","deposition_date":"2024-10-29","release_date":"2025-02-03","obsolete_date":null,"update_date":"2025-02-03","corresponding_author":{"author":{"author_orcid":"0000-0001-5582-2851","middle_name":null,"organization":"International Institute of Molecular and Cell Biology in Warsaw","street":"4 Ks. Trojdena Street","town_or_city":"Warsaw","state_or_province":null,"post_or_zip":"02-109","first_name":"Mariusz","last_name":"Czarnocki-Cieciura","country":"Poland"}},"authors":[{"author":{"name":"Palchevskyi S","author_orcid":"0000-0001-6553-1513"}},{"author":{"name":"Czarnocki-Cieciura M","author_orcid":"0000-0001-5582-2851"}},{"author":{"name":"Vistoli G","author_orcid":"0000-0002-3939-5172"}},{"author":{"name":"Gervasoni S","author_orcid":"0000-0002-5731-3396"}},{"author":{"name":"Nowak E","author_orcid":"0000-0002-1466-898X"}},{"author":{"name":"Beccari AR","author_orcid":"0000-0001-6830-2695"}},{"author":{"name":"Nowotny M","author_orcid":"0000-0001-8632-0977"}},{"author":{"name":"Talarico C","author_orcid":"0000-0003-4789-0955"}}],"cross_references":["EMD-15983","EMD-15982","EMD-15981"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Palchevskyi S","author_orcid":"0000-0001-6553-1513"},{"name":"Czarnocki-Cieciura M","author_orcid":"0000-0001-5582-2851"},{"name":"Vistoli G","author_orcid":"0000-0002-3939-5172"},{"name":"Gervasoni S","author_orcid":"0000-0002-5731-3396"},{"name":"Nowak E","author_orcid":"0000-0002-1466-898X"},{"name":"Beccari AR","author_orcid":"0000-0001-6830-2695"},{"name":"Nowotny M","author_orcid":"0000-0001-8632-0977"},{"name":"Talarico C","author_orcid":"0000-0003-4789-0955"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structure of human TRPM8 channel","volume":"6","country":"","first_page":null,"last_page":null,"year":"2023","language":"English","doi":"10.1038/s42003-023-05425-6","pubmedid":"37857704","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Communications biology","journal_abbreviation":"Commun Biol","issue":"1","preprint":false}],"dataset_size":"3.4 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["8bdc"],"entry_doi":"10.6019/EMPIAR-12443"}}