{"EMPIAR-12405":{"imagesets":[{"segmentations":[],"name":"Dataset 1","directory":"data/Beckman_02222023","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":2945,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.54,"pixel_height":0.54,"micrographs_file_pattern":"data/Beckman_02222023/movies/*tiff","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Dataset 1. Super-resolution movies (binned 2x during motion correction). Total dose = 45.6 electrons/Å^2. The gain reference is also provided as a .mrc file in data/Beckman_02222023/gainref.","image_width":"8184","image_height":"11520"},{"segmentations":[],"name":"Dataset 2","directory":"data/Beckman_03092023","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":6806,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.54,"pixel_height":0.54,"micrographs_file_pattern":"data/Beckman_03092023/movies/*tiff","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Dataset 2. Super-resolution movies (binned 2x during motion correction). Total dose = 43.7 electrons/Å^2. The gain reference is also provided as a .mrc file in data/Beckman_03092023/gainref.","image_width":"8184","image_height":"11520"},{"segmentations":[],"name":"Dataset 3","directory":"data/Beckman_04112023","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":3682,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.54,"pixel_height":0.54,"micrographs_file_pattern":"data/Beckman_04112023/movies/*tiff","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Dataset 3. Super-resolution movies (binned 2x during motion correction). Total dose = 43.7 electrons/Å^2. The gain reference is also provided as a .mrc file in data/Beckman_04112023/gainref.","image_width":"8184","image_height":"11520"},{"segmentations":[],"name":"Dataset 4","directory":"data/Beckman_04122023","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":5963,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.54,"pixel_height":0.54,"micrographs_file_pattern":"data/Beckman_04122023/movies*/*tiff","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Dataset 4. Super-resolution movies (binned 2x during motion correction). Total dose = 43.7 electrons/Å^2. The gain reference is also provided as a .mrc file in data/Beckman_04122023/gainref.","image_width":"8184","image_height":"11520"},{"segmentations":[],"name":"Dataset 5","directory":"data/Beckman_07022023","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":7814,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.54,"pixel_height":0.54,"micrographs_file_pattern":"data/Beckman_07022023/movies*/*tiff","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Dataset 5. Super-resolution movies (binned 2x during motion correction). Total dose = 44 electrons/Å^2. The gain reference is also provided as a .mrc file in data/Beckman_07022023/gainref.","image_width":"8184","image_height":"11520"},{"segmentations":[],"name":"Mg-F-actin Severed Dataset","directory":"data/Beckman_04052024","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":4594,"frames_per_image":37,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.54,"pixel_height":0.54,"micrographs_file_pattern":"data/Beckman_04052024/movies*/*tiff","picked_particles_file_pattern":"","picked_particles_directory":"","details":"PLEASE NOTE: This dataset was not used to generate the associated EMDs and PDBs. This dataset was collected under different sample preparations conditions (Mg-F-actin instead of Ca-G-actin) to demonstrate F-actin severing by gelsolin. The grids used for this dataset were also different (2nm ultra-thin carbon), resulting in a minor orientation bias. Please see methods and Extended Data Fig. 4 in the publication (doi.org/10.1038/s41594-024-01412-5) for more details.\n\nSuper-resolution movies (binned 2x during motion correction). Total dose = 45.3 electrons/Å^2. The gain reference is also provided as a .mrc file in data/Beckman_04052024/gainref.","image_width":"8184","image_height":"11520"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)","code":"GM073791 and GM152412","country":"United States"}],"version_history":[],"title":"Gelsolin-severed and capped F-actin raw data from 'Mechanism of actin filament severing and capping by gelsolin'","principal_investigator":[{"author_orcid":"0000-0003-3186-5229","middle_name":null,"organization":"Department of Physiology, Perelman School of Medicine, University of Pennsylvania","street":"415 Curie Blvd.","town_or_city":"Philadelphia","state_or_province":"PA","post_or_zip":"19104","telephone":null,"fax":null,"first_name":"Roberto","last_name":"Dominguez","email":"droberto [at] pennmedicine.upenn.edu","country":"United States","entry":"EMPIAR-12405"}],"status":"REL","deposition_date":"2024-06-13","release_date":"2024-12-09","obsolete_date":null,"update_date":"2024-12-09","corresponding_author":{"author":{"author_orcid":"0000-0003-3186-5229","middle_name":null,"organization":"Department of Physiology, Perelman School of Medicine, University of Pennsylvania","street":"415 Curie Blvd.","town_or_city":"Philadelphia","state_or_province":"PA","post_or_zip":"19104","first_name":"Roberto","last_name":"Dominguez","country":"United States"}},"authors":[{"author":{"name":"Barrie KR","author_orcid":"0000-0003-0860-1639"}},{"author":{"name":"Rebowski G","author_orcid":"0000-0002-0766-6302"}},{"author":{"name":"Dominguez R","author_orcid":"0000-0003-3186-5229"}}],"cross_references":["EMD-43264","EMD-43268","EMD-43263","EMD-43262","EMD-43274","EMD-43316"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Barrie KR","author_orcid":"0000-0003-0860-1639"},{"name":"Rebowski G","author_orcid":"0000-0002-0766-6302"},{"name":"Dominguez R","author_orcid":"0000-0003-3186-5229"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Mechanism of actin filament severing and capping by gelsolin","volume":null,"country":"","first_page":null,"last_page":null,"year":"2024","language":"English","doi":"10.1038/s41594-024-01412-5","pubmedid":"39448849","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature structural & molecular biology","journal_abbreviation":"Nat Struct Mol Biol","issue":null,"preprint":false}],"dataset_size":"13.3 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["8viz","8vkh"],"entry_doi":"10.6019/EMPIAR-12405"}}