{"EMPIAR-12330":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame movies of the yeast P-complex spliceosome, 81000x magnification","directory":"data/movies_grid1","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":15690,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.934,"pixel_height":0.934,"micrographs_file_pattern":"data/movies_grid1/movies/GridSquare_*/Data/FoilHole_*_fractions.tiff","picked_particles_file_pattern":"data/stateI_emd47157.star","picked_particles_directory":"","details":"Data for grid 1, collected at the MRC LMB Krios1\n\nVoltage\t\t300 keV\nCamera\t\tGatan K3, super-resolution mode with 2-fold binning by EPU\nEnergy filter\tGIF, 20eV slit width\nMagnification\t81000\nPixel size\t0.934 A/pix\nDose\t\t40 e/A2\nExposure rate\t25 e/pix/s\nExposure time\t1.44s\nFractions\t40\tby mistake, some of the movies have 41 frames instead. Depending on your processing strategy, these might have to be removed or processed seperately to avoid bugs.\n\nThe gain reference \"gainref.tiff\" is provided. It can be used without rotation or flipping for RELION motion correction.\n\nMetadata are also included for all maps deposited to the EMDB from this dataset. These are direct copies of the \"run_data.star\" from the Refine3D directory of the RELION project.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Unaligned multi-frame movies of the yeast P-complex spliceosome, 130000x magnification","directory":"data/movies_grid2","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":35423,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.664,"pixel_height":0.664,"micrographs_file_pattern":"data/movies_grid2/movies/GridSquare_*/Data/FoilHole_*_fractions.tiff","picked_particles_file_pattern":"data/stateI_emd47157.star","picked_particles_directory":"","details":"Data for grid 2, collected at the MRC LMB Krios3\n\nVoltage\t\t300 keV\nCamera\t\tGatan K3, super-resolution mode with 2-fold binning by EPU\nEnergy filter\tGIF, 20eV slit width\nMagnification\t130000\nPixel size\t0.664 A/pix\nDose\t\t40 e/A2\nExposure rate\t25 e/pix/s\nExposure time\t0.72s\nFractions\t40\n\nThe gain reference \"gainref.tiff\" is provided. It can be used without rotation or flipping for RELION motion correction.\n\nMetadata are also included for all maps deposited to the EMDB from this dataset. These are direct copies of the \"run_data.star\" from the Refine3D directory of the RELION project.","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[{"funding_body":"Howard Hughes Medical Institute (HHMI)","code":"Helen Hay Whitney Postdoctoral Fellowship","country":"United States"}],"version_history":[],"title":"Yeast P complex spliceosome","principal_investigator":[{"author_orcid":"0000-0003-4738-9503","middle_name":null,"organization":"Massachusetts Institute of Technology","street":"75 Ames Street","town_or_city":"Cambridge","state_or_province":"Massachusetts","post_or_zip":"02142","telephone":null,"fax":null,"first_name":"Max","last_name":"Wilkinson","email":"mwilkin [at] mit.edu","country":"United States","entry":"EMPIAR-12330"}],"status":"REL","deposition_date":"2024-10-04","release_date":"2025-04-24","obsolete_date":null,"update_date":"2025-04-24","corresponding_author":{"author":{"author_orcid":"0000-0003-4738-9503","middle_name":null,"organization":"Massachusetts Institute of Technology","street":"75 Ames Street","town_or_city":"Cambridge","state_or_province":"Massachusetts","post_or_zip":"02142","first_name":"Max","last_name":"Wilkinson","country":"United States"}},"authors":[{"author":{"name":"Wilkinson ME","author_orcid":"0000-0003-4738-9503"}},{"author":{"name":"Hoskins AA","author_orcid":"0000-0002-9777-519X"}}],"cross_references":["EMD-47158","EMD-47161","EMD-47157","EMD-47163","EMD-47162","EMD-47164","EMD-47159","EMD-47160"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Senn KA","author_orcid":"0000-0003-2172-7113"},{"name":"Lipinski KA","author_orcid":"0000-0002-4271-1586"},{"name":"Zeps NJ","author_orcid":null},{"name":"Griffin AF","author_orcid":"0009-0004-0976-3749"},{"name":"Wilkinson ME","author_orcid":"0000-0003-4738-9503"},{"name":"Hoskins AA","author_orcid":"0000-0002-9777-519X"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Control of 3’ splice site selection by the yeast splicing factor Fyv6","volume":null,"country":"United States","first_page":null,"last_page":null,"year":"2024","language":null,"doi":"10.1101/2024.05.04.592262","pubmedid":"38746449","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"bioRxiv","journal_abbreviation":"Mol Cell","issue":"7","preprint":true}],"dataset_size":"7.0 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9dtr"],"entry_doi":"10.6019/EMPIAR-12330"}}