{"EMPIAR-12328":{"imagesets":[{"segmentations":[],"name":"unaligned multi-frame movies of RNAP-TopoI on short-overhang scaffold","directory":"data/movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":7254,"frames_per_image":39,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.862,"pixel_height":0.862,"micrographs_file_pattern":"data/movies/movies-*/EC13-6*","picked_particles_file_pattern":"","picked_particles_directory":"data/particles/","details":"300 kV, Cs 0.01 mm, defocus range -1.0 – -2.0 um, total dose 55.55 e/A2\nGain reference file is provided in data/movies/gain.mrc","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"picked particles of RNAP-TopoI on short-overhang scaffold","directory":"data/particles","category":"picked particles - single frame - unprocessed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":7250,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.862,"pixel_height":0.862,"micrographs_file_pattern":"data/movies/movies-*/EC13-6*","picked_particles_file_pattern":"","picked_particles_directory":"data/particles/","details":"Particles were picked and extracted in WARP (Tegunov & Cramer, Nat. Methods 2019) as a part of on-the-fly processing pipeline at the IGBMC, Strasbourg, France.","image_width":"360","image_height":"360"}],"workflow_file":null,"grant_references":[{"funding_body":"Agence Nationale de la Recherche (ANR)","code":"ANR-21-CE11-0040-01","country":"France"},{"funding_body":"Agence Nationale de la Recherche (ANR)","code":"ANR-10-INBS-05","country":"France"}],"version_history":[],"title":"RNAP-TopoI complex on short-overhang scaffold","principal_investigator":[{"author_orcid":"0000-0001-7793-4029","middle_name":null,"organization":"Department of integrative Structural Biology, IGBMC","street":null,"town_or_city":"Illkirch","state_or_province":null,"post_or_zip":"67404","telephone":null,"fax":null,"first_name":"Valerie","last_name":"Lamour","email":"lamourv [at] igbmc.fr","country":"France","entry":"EMPIAR-12328"},{"author_orcid":"0000-0001-9635-3844","middle_name":null,"organization":"Department of Integrated Structural Biology, Institute of Genetics and Molecular and Cellular Biology - IGBMC - UMR 7104 - U 1258","street":"1, rue Laurent Fries","town_or_city":"Illkirch","state_or_province":null,"post_or_zip":"67404","telephone":null,"fax":null,"first_name":"Albert","last_name":"Weixlbaumer","email":"Albert.Weixlbaumer [at] igbmc.fr","country":"France","entry":"EMPIAR-12328"}],"status":"REL","deposition_date":"2024-10-03","release_date":"2025-11-18","obsolete_date":null,"update_date":"2025-11-18","corresponding_author":{"author":{"author_orcid":"0000-0001-9635-3844","middle_name":null,"organization":"Department of Integrated Structural Biology, Institute of Genetics and Molecular and Cellular Biology - IGBMC - UMR 7104 - U 1258","street":"1, rue Laurent Fries","town_or_city":"Illkirch","state_or_province":null,"post_or_zip":"67404","first_name":"Albert","last_name":"Weixlbaumer","country":"France"}},"authors":[{"author":{"name":"Vidmar VV","author_orcid":"0000-0002-2809-5596"}},{"author":{"name":"Lamour VL","author_orcid":"0000-0001-7793-4029"}},{"author":{"name":"Weixlbaumer AW","author_orcid":"0000-0001-9635-3844"}}],"cross_references":["EMD-51271","EMD-51270"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Vidmar VV","author_orcid":"0000-0002-2809-5596"},{"name":"Borde CB","author_orcid":"0009-0002-1528-7342"},{"name":"Bruno LB","author_orcid":null},{"name":"Takacs MT","author_orcid":"0000-0001-8616-8418"},{"name":"Batisse CB","author_orcid":"0000-0003-3064-6569"},{"name":"Saint-Andre CSA","author_orcid":null},{"name":"Zhu CZ","author_orcid":"0000-0002-9573-6170"},{"name":"Espeli OE","author_orcid":"0000-0003-2804-2192"},{"name":"Lamour VL","author_orcid":"0000-0001-7793-4029"},{"name":"Weixlbaumer AW","author_orcid":"0000-0001-9635-3844"}],"editors":[],"published":false,"j_or_nj_citation":true,"title":"DNA topoisomerase I acts as supercoiling sensor for transcription elongation in E. coli","volume":null,"country":"","first_page":null,"last_page":null,"year":null,"language":null,"doi":null,"pubmedid":null,"details":"The micrographs are raw movies (39 frames) without gain correction (gain.mrc is provided); total electron dose is 55.55 e/A2.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"","journal_abbreviation":"","issue":null,"preprint":true}],"dataset_size":"1.5 TB","experiment_type":"EMDB","scale":"molecule","entry_doi":"10.6019/EMPIAR-12328"}}