{"EMPIAR-12326":{"imagesets":[{"segmentations":[],"name":"unaligned multi-frame movies of RNAP-TopoI on long-overhang scaffold","directory":"data/movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":16045,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.645,"pixel_height":0.645,"micrographs_file_pattern":"data/movies/movies_*/210409_boxG-grid2_*.tif","picked_particles_file_pattern":"","picked_particles_directory":"data/particles/","details":"300 kV, Cs 2.7 mm, defocus range -0.8 – -2.0 um, total dose 49.91 e/A2\nGain reference file is provided in data/movies/gain.mrc","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"all good particles of RNAP-TopoI on long-overhang scaffold","directory":"data/particles","category":"picked particles - single frame - processed","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.9987,"pixel_height":0.9987,"micrographs_file_pattern":"data/movies/movies_*/210409_boxG-grid2_*.tif","picked_particles_file_pattern":"","picked_particles_directory":"data/particles/","details":"Particles were initially picked in crYOLO (Wagner et al., Commun. Biol. 2019) and re-extracted after 2D classification & heterogeneous refinement in cryoSPARC (Punjani et al., Nat. Methods 2017)","image_width":"310","image_height":"310"}],"workflow_file":null,"grant_references":[{"funding_body":"Agence Nationale de la Recherche (ANR)","code":"ANR-21-CE11-0040-01","country":"France"},{"funding_body":"Agence Nationale de la Recherche (ANR)","code":"ANR-10-INBS-05","country":"France"}],"version_history":[],"title":"RNAP-TopoI complex on long-overhang scaffold","principal_investigator":[{"author_orcid":"0000-0001-7793-4029","middle_name":null,"organization":"Department of integrative Structural Biology, IGBMC","street":null,"town_or_city":"Illkirch","state_or_province":null,"post_or_zip":"67404","telephone":null,"fax":null,"first_name":"Valerie","last_name":"Lamour","email":"lamourv [at] igbmc.fr","country":"France","entry":"EMPIAR-12326"},{"author_orcid":"0000-0001-9635-3844","middle_name":null,"organization":"Department of Integrated Structural Biology, Institute of Genetics and Molecular and Cellular Biology - IGBMC - UMR 7104 - U 1258","street":"1, rue Laurent Fries","town_or_city":"Illkirch","state_or_province":null,"post_or_zip":"67404","telephone":null,"fax":null,"first_name":"Albert","last_name":"Weixlbaumer","email":"Albert.Weixlbaumer [at] igbmc.fr","country":"France","entry":"EMPIAR-12326"}],"status":"REL","deposition_date":"2024-10-03","release_date":"2025-11-19","obsolete_date":null,"update_date":"2025-11-19","corresponding_author":{"author":{"author_orcid":"0000-0001-9635-3844","middle_name":null,"organization":"Department of Integrated Structural Biology, Institute of Genetics and Molecular and Cellular Biology - IGBMC - UMR 7104 - U 1258","street":"1, rue Laurent Fries","town_or_city":"Illkirch","state_or_province":null,"post_or_zip":"67404","first_name":"Albert","last_name":"Weixlbaumer","country":"France"}},"authors":[{"author":{"name":"Vidmar VV","author_orcid":"0000-0002-2809-5596"}},{"author":{"name":"Lamour VL","author_orcid":"0000-0001-7793-4029"}},{"author":{"name":"Weixlbaumer AW","author_orcid":"0000-0001-9635-3844"}}],"cross_references":["EMD-51269","EMD-51264","EMD-51268","EMD-51263"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Vidmar VV","author_orcid":"0000-0002-2809-5596"},{"name":"Borde CB","author_orcid":"0009-0002-1528-7342"},{"name":"Bruno LB","author_orcid":null},{"name":"Takacs MT","author_orcid":"0000-0001-8616-8418"},{"name":"Batisse CB","author_orcid":"0000-0003-3064-6569"},{"name":"Saint-Andre CSA","author_orcid":null},{"name":"Zhu CZ","author_orcid":"0000-0002-9573-6170"},{"name":"Espeli OE","author_orcid":"0000-0003-2804-2192"},{"name":"Lamour VL","author_orcid":"0000-0001-7793-4029"},{"name":"Weixlbaumer AW","author_orcid":"0000-0001-9635-3844"}],"editors":[],"published":false,"j_or_nj_citation":true,"title":"DNA topoisomerase I acts as supercoiling sensor for transcription elongation in E. coli","volume":null,"country":"","first_page":null,"last_page":null,"year":null,"language":null,"doi":null,"pubmedid":null,"details":"The micrographs are raw movies (40 frames) without gain correction (gain.mrc is provided); total electron dose is 49.91 e/A2","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"","journal_abbreviation":"","issue":null,"preprint":true}],"dataset_size":"2.5 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9gdh","9gde"],"entry_doi":"10.6019/EMPIAR-12326"}}