{"EMPIAR-12323":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of the µ-opioid receptor bound to a nanobody antagonist NbE","directory":"data","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":5939,"frames_per_image":44,"frame_range_min":1,"frame_range_max":44,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":0.9759,"pixel_height":0.9759,"micrographs_file_pattern":"data/12248/data/FoilHole_*_Data_*_*_*_*_Fractions.mrc","picked_particles_file_pattern":"","picked_particles_directory":"","details":"1. All the images are raw data.\n\n2. All the images are multi-frame micrographs composed of 44 frames each in MRC format.\n\n3. All the images are acquired on a Thermo Scientific Talos Arctica Cryo-TEM at an accelerating voltage of 200 kV using a Falcon III direct electron detector at a nominal\nmagnification of 150,000x, resulting in a pixel size of 0.9759 Å. \n\n4. Data were collected using EPU  with a defocus range of −0.6 to −2.0 μm and a total electron dose of 40 e−/Å2","image_width":"4096","image_height":"4096"}],"workflow_file":null,"grant_references":[{"funding_body":"Swiss National Science Foundation","code":"TMSGI3_211581","country":"Switzerland"},{"funding_body":"Swiss National Science Foundation","code":"310030185235","country":"Switzerland"},{"funding_body":"Swiss National Science Foundation","code":"PCEFP3_181282","country":"Switzerland"}],"version_history":[],"title":"Unaligned multi-frame micrographs of the mu opioid receptor bound to a nanobody antagonist NbE","principal_investigator":[{"author_orcid":"0000-0003-1218-6714","middle_name":null,"organization":"Department of molecular and cellular biology, Faculty of Science, University of Geneva, Switzerland","street":"30, quai Ernest-Ansermet","town_or_city":"Geneva","state_or_province":"Geneva","post_or_zip":"1211","telephone":null,"fax":null,"first_name":"Andreas","last_name":"Boland","email":"Andreas.Boland [at] unige.ch","country":"Switzerland","entry":"EMPIAR-12323"},{"author_orcid":"0000-0002-5210-2864","middle_name":null,"organization":"Department of Cell Physiology and Metabolism, University of Geneva, Geneva, Switzerland","street":"1 rue Michel-Servet","town_or_city":"Geneva","state_or_province":"Geneva","post_or_zip":"1211","telephone":null,"fax":null,"first_name":"Miriam","last_name":"Stoeber","email":"miriam.stoeber [at] unige.ch","country":"Switzerland","entry":"EMPIAR-12323"}],"status":"REL","deposition_date":"2024-09-04","release_date":"2025-01-09","obsolete_date":null,"update_date":"2025-01-09","corresponding_author":{"author":{"author_orcid":"0000-0001-5642-3991","middle_name":null,"organization":"Departmemt of molecular and cellular biology, facuty of science, University of Geneva, Switzerland","street":"30, quai Ernest-Ansermet","town_or_city":"Geneva","state_or_province":"Geneva","post_or_zip":"1211","first_name":"Jun","last_name":"Yu","country":"Switzerland"}},"authors":[{"author":{"name":"Yu J","author_orcid":"0000-0001-5642-3991"}},{"author":{"name":"Kumar A","author_orcid":"0009-0008-2950-7792"}},{"author":{"name":"Zhang X","author_orcid":"0000-0002-5222-7042"}},{"author":{"name":"Martin C","author_orcid":"0000-0001-7368-9142"}},{"author":{"name":"Holsbeeck KV","author_orcid":null}},{"author":{"name":"Raia P","author_orcid":"0000-0003-3469-1922"}},{"author":{"name":"Koehl A","author_orcid":null}},{"author":{"name":"Laeremans T","author_orcid":null}},{"author":{"name":"Steyaert J","author_orcid":null}},{"author":{"name":"Manglik A","author_orcid":"0000-0002-7173-3741"}},{"author":{"name":"Ballet S","author_orcid":"0000-0003-4123-1641"}},{"author":{"name":"Boland A","author_orcid":"0000-0003-1218-6714"}},{"author":{"name":"Stoeber M","author_orcid":"0000-0002-5210-2864"}}],"cross_references":["EMD-18541"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Yu J","author_orcid":null},{"name":"Kumar A","author_orcid":null},{"name":"Zhang X","author_orcid":"0000-0002-5222-7042"},{"name":"Martin C","author_orcid":null},{"name":"Van Holsbeeck K","author_orcid":"0000-0002-9345-4962"},{"name":"Raia P","author_orcid":"0000-0003-3469-1922"},{"name":"Koehl A","author_orcid":null},{"name":"Laeremans T","author_orcid":null},{"name":"Steyaert J","author_orcid":"0000-0002-3825-874X"},{"name":"Manglik A","author_orcid":"0000-0002-7173-3741"},{"name":"Ballet S","author_orcid":"0000-0003-4123-1641"},{"name":"Boland A","author_orcid":"0000-0003-1218-6714"},{"name":"Stoeber M","author_orcid":"0000-0002-5210-2864"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structural basis of μ-opioid receptor targeting by a nanobody 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