{"EMPIAR-12322":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame movies of DRT2 ribonucleoprotein complex in the resting state (no added dNTPs)","directory":"data/movies_resting","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":28211,"frames_per_image":41,"frame_range_min":1,"frame_range_max":41,"voxel_type":"UNSIGNED BYTE","pixel_width":0.663,"pixel_height":0.663,"micrographs_file_pattern":"data/movies_resting/grid*/movies_grid*/GridSquare_*/Data/FoilHole_*_fractions.tiff","picked_particles_file_pattern":"data/particles_resting.star","picked_particles_directory":"","details":"Data collected at MIT.nano on Thermo Scientific Titan Krios G3i cryo-TEM\n\nVoltage\t\t300 keV\nCamera\t\tGatan K3, super-resolution mode with 2-fold binning by EPU\nEnergy filter\tGIF, 20eV slit width\nMagnification\t130000\nPixel size\t0.663 A/pix\nDose\t\t40 e/A2\nExposure rate\t19.9 e/pix/s\nExposure time\t0.9 s\nFractions\t40\n\nTotal mics\t14220 (grid 1)\n\t\t13991 (grid 2)\n\n\nEach grid has its own \"gainref.mrc\" gain reference file. These weres binned using 'rebin by two' in Digital Micrograph. They need to be flipped for use in RELION motion correction (Gain flip: Flip upside down (1) ). It can be used as is for cryoSPARC.\n\nparticles_resting.star contains metadata for the 230,001 particles refined for the resting state (EMDB 45085).","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Unaligned multi-frame movies of DRT2 ribonucleoprotein complex in the elongating state (+dNTPs)","directory":"data/movies_elongating","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":18453,"frames_per_image":41,"frame_range_min":1,"frame_range_max":41,"voxel_type":"UNSIGNED BYTE","pixel_width":0.663,"pixel_height":0.663,"micrographs_file_pattern":"data/movies_elongating/movies/GridSquare_*/Data/FoilHole_*_fractions.tiff","picked_particles_file_pattern":"data/particles_elongating.star","picked_particles_directory":"","details":"Data collected at MIT.nano on Thermo Scientific Titan Krios G3i cryo-TEM\n\nVoltage\t\t300 keV\nCamera\t\tGatan K3, super-resolution mode with 2-fold binning by EPU\nEnergy filter\tGIF, 20eV slit width\nMagnification\t130000\nPixel size\t0.663 A/pix\nDose\t\t40 e/A2\nExposure rate\t20.4\nExposure time 0.87 s\nFractions\t40\n\nTotal mics\t18453\n\nEach grid has its own \"gainref.mrc\" gain reference file. These were binned using 'rebin by two' in Digital Micrograph. They need to be flipped for use in RELION motion correction (Gain flip: Flip upside down (1) ). It can be used as is for cryoSPARC.\n\nparticles_elongating.star contains metadata for the 136,601 particles refined for the elongating state (EMDB 45086).","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[{"funding_body":"Howard Hughes Medical Institute (HHMI)","code":"NA","country":"United States"},{"funding_body":"National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)","code":"R01HG009761","country":"United States"}],"version_history":[],"title":"Cryo-EM micrographs for the DRT2 reverse transcriptase / ncRNA complex in resting and elongating states","principal_investigator":[{"author_orcid":"0000-0003-0178-7995","middle_name":null,"organization":"Broad Institute of MIT and Harvard","street":null,"town_or_city":"Cambridge","state_or_province":null,"post_or_zip":"02142","telephone":null,"fax":null,"first_name":"Feng","last_name":"Zhang","email":"zhang [at] broadinstitute.org","country":"United States","entry":"EMPIAR-12322"}],"status":"REL","deposition_date":"2024-10-01","release_date":"2025-06-02","obsolete_date":null,"update_date":"2025-06-02","corresponding_author":{"author":{"author_orcid":"0000-0003-4738-9503","middle_name":null,"organization":"Broad Institute of MIT and Harvard","street":"75 Ames St","town_or_city":"Cambridge","state_or_province":"Massachusetts","post_or_zip":"02142","first_name":"Max","last_name":"Wilkinson","country":"United States"}},"authors":[{"author":{"name":"Wilkinson ME","author_orcid":"0000-0003-4738-9503"}},{"author":{"name":"Li D","author_orcid":"0000-0002-8921-5168"}},{"author":{"name":"Gao A","author_orcid":"0000-0002-3579-0327"}},{"author":{"name":"Macrae RK","author_orcid":"0000-0002-7850-9056"}},{"author":{"name":"Zhang F","author_orcid":"0000-0003-0178-7995"}}],"cross_references":["EMD-45085","EMD-45086"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Wilkinson ME","author_orcid":"0000-0003-4738-9503"},{"name":"Li D","author_orcid":"0000-0002-8921-5168"},{"name":"Gao A","author_orcid":"0000-0002-3579-0327"},{"name":"Macrae RK","author_orcid":"0000-0002-7850-9056"},{"name":"Zhang F","author_orcid":"0000-0003-0178-7995"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Phage-triggered reverse transcription assembles a toxic repetitive gene from a noncoding RNA","volume":null,"country":"","first_page":null,"last_page":null,"year":"2024","language":"English","doi":"10.1126/science.adq3977","pubmedid":"39208082","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Science (New York, N.Y.)","journal_abbreviation":"Science","issue":null,"preprint":false}],"dataset_size":"6.2 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9c0i","9c0j"],"entry_doi":"10.6019/EMPIAR-12322"}}