{"EMPIAR-12298":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of ΔCag3 Cag Type 4 Secretion systems of Helicobacter pylori","directory":"data","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":3373,"frames_per_image":40,"frame_range_min":1,"frame_range_max":40,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":1.0,"pixel_height":1.0,"micrographs_file_pattern":"data/*.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"","image_width":"3838","image_height":"3710"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health (NIH)","code":"AI118932","country":"United States"},{"funding_body":"National Institutes of Health (NIH)","code":"CA116087","country":"United States"},{"funding_body":"National Institutes of Health (NIH)","code":"AI039657","country":"United States"},{"funding_body":"National Institutes of Health (NIH)","code":"GM103310","country":"United States"},{"funding_body":"National Institutes of Health (NIH)","code":"2T32DK007673","country":"United States"},{"funding_body":"National Institutes of Health (NIH)","code":"S10OD020011","country":"United States"},{"funding_body":"Department of Veterans Affairs (VA, United States)","code":"5I01BX004447","country":"United States"}],"version_history":[],"title":"Movies of ΔCag3 Mutant Cag T4SS from Helicobacter pylori","principal_investigator":[{"author_orcid":"0000-0003-1750-4793","middle_name":null,"organization":"Life Sciences Institute, University of Michigan","street":null,"town_or_city":"Ann Arbor","state_or_province":"Michigan","post_or_zip":"48109","telephone":null,"fax":null,"first_name":"Melanie","last_name":"Ohi","email":"mohi [at] umich.edu","country":"United States","entry":"EMPIAR-12298"}],"status":"REL","deposition_date":"2024-08-12","release_date":"2025-03-20","obsolete_date":null,"update_date":"2025-03-20","corresponding_author":{"author":{"author_orcid":"0000-0003-1750-4793","middle_name":null,"organization":"Life Sciences Institute, University of Michigan","street":null,"town_or_city":"Ann Arbor","state_or_province":"Michigan","post_or_zip":"48109","first_name":"Melanie","last_name":"Ohi","country":"United States"}},"authors":[{"author":{"name":"Sheedlo MJ","author_orcid":"0000-0002-3185-1727"}},{"author":{"name":"Chung JM","author_orcid":"0000-0002-4285-8764"}},{"author":{"name":"Sawhney N","author_orcid":"0000-0002-4943-1018"}},{"author":{"name":"Durie CL","author_orcid":"0000-0002-4027-4386"}},{"author":{"name":"Cover TL","author_orcid":"0000-0001-8503-002X"}},{"author":{"name":"Ohi MD","author_orcid":"0000-0003-1750-4793"}},{"author":{"name":"Lacy DB","author_orcid":"0000-0003-2273-8121"}}],"cross_references":["EMD-22076","EMD-22077"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Sheedlo MJ","author_orcid":"0000-0002-3185-1727"},{"name":"Chung JM","author_orcid":"0000-0002-4285-8764"},{"name":"Sawhney N","author_orcid":"0000-0002-4943-1018"},{"name":"Durie CL","author_orcid":"0000-0002-4027-4386"},{"name":"Cover TL","author_orcid":"0000-0001-8503-002X"},{"name":"Ohi MD","author_orcid":"0000-0003-1750-4793"},{"name":"Lacy DB","author_orcid":"0000-0003-2273-8121"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Cryo-EM reveals species-specific components within the <i>Helicobacter pylori</i> Cag type IV secretion system core complex","volume":"9","country":"","first_page":null,"last_page":null,"year":"2020","language":"English","doi":"10.7554/elife.59495","pubmedid":"32876048","details":"All the images were collected on a Thermo Fisher 300 kV Titan Krios electron microscope equipped with a Gatan K2 Summit Direct Electron Detector. The nominal pixel sizes for ΔCag3 samples were 1.00 Å per pixel (x29,000 magnification). The total exposure time was 8 s, and frames were recorded every 0.2 s, resulting in a total accumulated dose of 59.7 e−Å−2. Different defocus ranges were −1.5 to −3.5 μm.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"eLife","journal_abbreviation":"Elife","issue":null,"preprint":false}],"dataset_size":"688.7 GB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["6x6k","6x6l"],"entry_doi":"10.6019/EMPIAR-12298"}}