{"EMPIAR-12270":{"imagesets":[{"segmentations":[{"file":"data/210929_HRV2_60mpi_tomo7_segmentation/210929_tomo7_Segmentation","description":"Cryo electron tomography of Rhinovirus 2-infected cos-7 cell, vitrified 60 min. post-infection. The positions of virions, empty particles, ribosomes, and microfilaments were identified using template matching as implemented in emClarity (Himes et al., 2018, Nat Methods 15). Microfilaments and membranes were segmented manually.","original_files":null,"original_format":"MRC","entry":null}],"name":"Cryo electron tomography of Rhinovirus 2-infected cells, segmentation","directory":"data","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":null,"pixel_height":null,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Tilt series:\n- pixel width and height: 5.532 Å\n- Number of tilts: 41\n- number of columns, rows: 3838, 3710\n- dose-symmetric tilt scheme, ± 50°, 2.5° tilt increment\n- rawtlt file available\nTomograms:\n- pixel width and height: 16.60 Å (tomo7_full_rec.mrc), 22.13 Å (tomo7_full_rec_crop.mrc), tomo7_full_rec_crop.mrc used for segmentation","image_width":null,"image_height":null}],"workflow_file":null,"grant_references":[{"funding_body":"Czech Science Foundation","code":"GX19-25982X","country":"Czech Republic"},{"funding_body":"National Institute of Virology and Bacteriology","code":"LX22NPO5103","country":"Czech Republic"}],"version_history":[],"title":"Cryo electron tomography of Rhinovirus 2-infected cos-7 cells","principal_investigator":[{"author_orcid":"0000-0003-4215-3315","middle_name":null,"organization":"Centre for Structural Biology – Central European Institute of Technology","street":"Kamenice 5","town_or_city":"Brno","state_or_province":null,"post_or_zip":"62500","telephone":null,"fax":null,"first_name":"Pavel","last_name":"Plevka","email":"pavel.plevka [at] ceitec.muni.cz","country":"Czech Republic","entry":"EMPIAR-12270"}],"status":"REL","deposition_date":"2024-09-05","release_date":"2024-11-20","obsolete_date":null,"update_date":"2024-11-20","corresponding_author":{"author":{"author_orcid":"0000-0003-0608-6530","middle_name":null,"organization":"Centre for Structural Biology – Central European Institute of Technology","street":"Kamenice 5","town_or_city":"Brno","state_or_province":null,"post_or_zip":"62500","first_name":"Aygul","last_name":"Ishemgulova","country":"Czech Republic"}},"authors":[{"author":{"name":"Ishemgulova A","author_orcid":"0000-0003-0608-6530"}},{"author":{"name":"Mukhamedova L","author_orcid":"0000-0002-7294-6714"}},{"author":{"name":"Trebichalská Z","author_orcid":"0000-0002-8534-4915"}},{"author":{"name":"Füzik T","author_orcid":"0000-0002-1190-0210"}}],"cross_references":["EMD-15711","EMD-15710"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Ishemgulova A","author_orcid":"0000-0003-0608-6530"},{"name":"Mukhamedova L","author_orcid":"0000-0002-7294-6714"},{"name":"Trebichalská Z","author_orcid":"0000-0002-8534-4915"},{"name":"Rájecká V","author_orcid":"0000-0001-5211-0365"},{"name":"Payne P","author_orcid":"0000-0002-2711-9453"},{"name":"Šmerdová L","author_orcid":"0000-0001-8043-0921"},{"name":"Moravcová J","author_orcid":"0000-0002-9146-6505"},{"name":"Hrebík D","author_orcid":"0000-0003-4568-0196"},{"name":"Buchta D","author_orcid":"0000-0001-6787-6355"},{"name":"Škubník K","author_orcid":"0000-0002-3216-4945"},{"name":"Füzik T","author_orcid":"0000-0002-1190-0210"},{"name":"Vaňáčová Š","author_orcid":"0000-0001-8807-0684"},{"name":"Nováček J","author_orcid":"0000-0003-4013-3898"},{"name":"Plevka P","author_orcid":"0000-0003-4215-3315"}],"editors":[],"published":false,"j_or_nj_citation":true,"title":"Endosome rupture enables enteroviruses to infect cells","volume":null,"country":"","first_page":null,"last_page":null,"year":null,"language":null,"doi":null,"pubmedid":null,"details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Communications Biology","journal_abbreviation":"Commun. Biol.","issue":null,"preprint":false}],"dataset_size":"4.2 GB","experiment_type":"EMDB","scale":"cell","related_pdb_entries":["8ay5","8ay4"],"entry_doi":"10.6019/EMPIAR-12270"}}