{"EMPIAR-12228":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame movies of B.megaterium gas vesicles","directory":"data/movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":4352,"frames_per_image":60,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED BYTE","pixel_width":0.685,"pixel_height":0.685,"micrographs_file_pattern":"data/movies/FoilHole_*_fractions*tiff","picked_particles_file_pattern":"data/manualpick_starfiles/*star","picked_particles_directory":"data/manualpick_starfiles","details":"Unaligned multi-frame movies of B.megaterium gas vesicles (concentation: OD500=3.1) in superresolution mode from a K3 detector. Pixelsize is 1.37A, superres pixelsize is 0.685A. This pixel size was used for processing and in the publication. Subsequent work established that the pixelsize was not perfectly calibrated and should be closer to 1.341A.","image_width":"11520","image_height":"8184"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo-EM images of B. megaterium gas vesicles in thin vitreous monolayers","principal_investigator":[{"author_orcid":"0000-0003-3721-5104","middle_name":"J","organization":"Department of Bionanoscience / TU Delft","street":"Van der Maasweg 9","town_or_city":"Delft","state_or_province":"Zuid-Holland","post_or_zip":"2629HZ","telephone":null,"fax":null,"first_name":"Arjen","last_name":"Jakobi","email":"a.jakobi [at] tudelft.nl","country":"Netherlands","entry":"EMPIAR-12228"}],"status":"REL","deposition_date":"2024-07-26","release_date":"2025-02-03","obsolete_date":null,"update_date":"2025-02-03","corresponding_author":{"author":{"author_orcid":"0000-0003-3721-5104","middle_name":"T","organization":"Department of Bionanoscience / TU Delft","street":"Van der Maasweg 9","town_or_city":"Delft","state_or_province":"Zuid-Holland","post_or_zip":"2629HZ","first_name":"Stefan","last_name":"Huber","country":"Netherlands"}},"authors":[{"author":{"name":"Huber ST","author_orcid":"0000-0003-3721-5104"}},{"author":{"name":"Jakobi AJ","author_orcid":"0000-0002-7761-2027"}}],"cross_references":["EMD-14238"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Huber ST","author_orcid":"0000-0003-3721-5104"},{"name":"Terwiel D","author_orcid":null},{"name":"Evers WH","author_orcid":null},{"name":"Maresca D","author_orcid":null},{"name":"Jakobi AJ","author_orcid":"0000-0002-7761-2027"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Cryo-EM structure of gas vesicles for buoyancy-controlled motility","volume":"186","country":"","first_page":"975","last_page":"986.e13","year":"2023","language":"English","doi":"10.1016/j.cell.2023.01.041","pubmedid":"36868215","details":"4351 super-resolution movies, 1.37A pixel size (later calibrated to ~1.34 using a newer dataset and comparison of helical parameters), 30 e/A2, 60 frames, defocus range from −0.25 to −1.25 μm. Star-file of manual picks of thin 34-42 nm tubes in RELION included.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Cell","journal_abbreviation":"Cell","issue":"5","preprint":false}],"dataset_size":"2.3 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["7r1c"],"entry_doi":"10.6019/EMPIAR-12228"}}