{"EMPIAR-12186":{"imagesets":[{"segmentations":[],"name":"Unaligned multiframes HA-bound to antibodies","directory":"data","category":"picked particles - multiframe - processed","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":13223,"frames_per_image":50,"frame_range_min":null,"frame_range_max":null,"voxel_type":"16 BIT FLOAT","pixel_width":0.835,"pixel_height":0.835,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Micrographs from two data collections collected with the same optics on a Titan Krios G2 cryo-TEM coupled with a Gatan K3 direct electron detector at an accelerating voltage of 300 kV and processed together. The micrographs are the average, without realignment, of raw movie frames collected at a pixel size of 0.835 Angstrom/pixel at an accumulated dose of 45.8 electrons per square Angstrom over a 2 s exposure.","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)","code":"75N93019C00052","country":"United States"}],"version_history":[],"title":"Cryo-EM structure of antibody TJ5-13 bound to H3 COBRA NG2 hemagglutinin","principal_investigator":[{"author_orcid":"0000-0003-4185-5673","middle_name":"M","organization":"Department of Biomolecular Engineering, University of California","street":"1156 High Street","town_or_city":"Santa Cruz","state_or_province":"California","post_or_zip":"95064","telephone":"+ 831 459 5157","fax":null,"first_name":"Rebecca","last_name":"DuBois","email":"rmdubois [at] ucsc.edu","country":"United States","entry":"EMPIAR-12186"}],"status":"REL","deposition_date":"2024-04-09","release_date":"2025-10-15","obsolete_date":null,"update_date":"2025-10-15","corresponding_author":{"author":{"author_orcid":"0000-0002-2447-146X","middle_name":"Vincent","organization":"Department of Biomolecular Engineering, University of California","street":"1156 High Street","town_or_city":"Santa Cruz","state_or_province":"California","post_or_zip":"95064","first_name":"John","last_name":"Dzimianski","country":"United States"}},"authors":[{"author":{"name":"Dzimianski JV","author_orcid":"0000-0002-2447-146X"}},{"author":{"name":"Cruz JM","author_orcid":"0000-0001-6817-9340"}},{"author":{"name":"Balasco Serrao VH","author_orcid":"0000-0002-9398-2941"}},{"author":{"name":"DuBois RM","author_orcid":"0000-0003-4185-5673"}}],"cross_references":["EMD-44305"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Dzimianski JV","author_orcid":"0000-0002-2447-146X"},{"name":"Nagashima KA","author_orcid":"0000-0002-7501-6651"},{"name":"Cruz JM","author_orcid":"0000-0001-6817-9340"},{"name":"Sautto GA","author_orcid":null},{"name":"O'Rourke SM","author_orcid":null},{"name":"Serrão VHB","author_orcid":null},{"name":"Ross TM","author_orcid":"0000-0003-1947-7469"},{"name":"Mousa JJ","author_orcid":"0000-0003-4709-2478"},{"name":"DuBois RM","author_orcid":"0000-0003-4185-5673"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Assessing the structural boundaries of broadly reactive antibody interactions with diverse H3 influenza hemagglutinin proteins","volume":null,"country":"United States","first_page":null,"last_page":null,"year":"2025","language":"English","doi":"10.1128/jvi.00453-25","pubmedid":"40810533","details":"Titan Krios G2 cryo-TEM coupled with a Gatan K3 direct electron detector at an accelerating voltage of 300 kV located at the W.M. Keck Foundation Advanced Microscopy Laboratory - UCSF. The data was collected in SerialEM in counting mode at a nominal magnification of 105,500, pixel size of 0.835 Å/pixel, exposure time of 2 s, and a total dose of 45.8 electrons/Å2. The data from each were combined for a total of 13,223 movies and processed together using CryoSPARC v3.2.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Journal of virology","journal_abbreviation":"J Virol","issue":null,"preprint":false}],"dataset_size":"29.6 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9b7g"],"entry_doi":"10.6019/EMPIAR-12186"}}