{"EMPIAR-12179":{"imagesets":[{"segmentations":[],"name":"SPOT-RASTR - a cryo-EM specimen preparation technique that overcomes problems with preferred orientation and the air/water interface","directory":"data","category":"picked particles - single frame - unprocessed","header_format":"MRC","data_format":"MRCS","num_images_or_tilt_series":230000,"frames_per_image":80,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.44,"pixel_height":1.44,"micrographs_file_pattern":"","picked_particles_file_pattern":"data/J256_stack.star","picked_particles_directory":"","details":"The particle stack is the picked lipid nanotube particles. The 3D volume is the tube azimuthal average to be subtracted from the picked particles for further processing based on the described protocol in the SPOT-RASTR paper.","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)","code":"GM148734","country":"United States"}],"version_history":[],"title":"SPOT-RASTR - a cryo-EM specimen preparation technique that overcomes problems with preferred orientation and the air/water interface","principal_investigator":[{"author_orcid":"0000-0001-5954-8933","middle_name":null,"organization":"us","street":"91 Chieftan Way","town_or_city":"Tallahassee","state_or_province":"FL","post_or_zip":"32304","telephone":"8503457886","fax":null,"first_name":"Behrouz","last_name":"Ghazi Esfahani","email":"behruzpino [at] gmail.com","country":"United States","entry":"EMPIAR-12179"}],"status":"REL","deposition_date":"2024-07-11","release_date":"2024-08-29","obsolete_date":null,"update_date":"2024-08-29","corresponding_author":{"author":{"author_orcid":"0000-0001-8855-4152","middle_name":null,"organization":"Florida State University","street":"91 Chieftan Way","town_or_city":"Tallahassee","state_or_province":"FL","post_or_zip":"32306-4380","first_name":"Scott","last_name":"Stagg","country":"United States"}},"authors":[{"author":{"name":"Ghazi Esfahani BGE","author_orcid":"0000-0001-5954-8933"}},{"author":{"name":"Randolph PR","author_orcid":"0000-0002-9884-2964"}},{"author":{"name":"Peng RP","author_orcid":"0000-0001-6057-8961"}},{"author":{"name":"Stroupe MES","author_orcid":"0000-0002-4393-1951"}},{"author":{"name":"Grant TG","author_orcid":"0000-0002-4855-8703"}},{"author":{"name":"Stagg SMS","author_orcid":"0000-0001-8855-4152"}}],"cross_references":["EMD-43514"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Esfahani BG","author_orcid":null},{"name":"Randolph PS","author_orcid":null},{"name":"Peng R","author_orcid":null},{"name":"Grant T","author_orcid":null},{"name":"Stroupe ME","author_orcid":null},{"name":"Stagg SM","author_orcid":"0000-0001-8855-4152"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"SPOT-RASTR - a cryo-EM specimen preparation technique that overcomes problems with preferred orientation and the air/water interface","volume":null,"country":"United States","first_page":null,"last_page":null,"year":"2024","language":"English","doi":"10.1101/2024.01.24.577038","pubmedid":"38501120","details":"The tube picks stack plus the azimuthal average to subtract.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"bioRxiv","journal_abbreviation":"PNAS Nexus","issue":null,"preprint":true}],"dataset_size":"304.6 GB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["8vt0"],"entry_doi":"10.6019/EMPIAR-12179"}}