{"EMPIAR-12176":{"imagesets":[{"segmentations":[],"name":"Raw frames from dataset 1 of microsporidian Encephalitozoon intestinalis","directory":"data/Data_Set_1","category":"tilt series","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":33,"frames_per_image":12,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":2.076,"pixel_height":2.077,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Each tilt series is contained within its unique directory with an identifiable name. The folder called 'raw_frames' contains the raw frames and corresponding mdoc files from each tilt series.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Unaligned tilt stacks from dataset 1 of microsporidian Encephalitozoon intestinalis","directory":"data/Data_Set_1","category":"tilt series","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":33,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":2.077,"pixel_height":2.077,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Each tilt series is contained within its unique directory with an identifiable name. The folder called 'tilt_series' contains the unaligned tilt stacks with corresponding tilt files. The aligned tilt series file can be found in 'aligned_tilt_series'.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Reconstructed tomograms from dataset 1 of microsporidian Encephalitozoon intestinalis","directory":"data/Data_Set_1","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":33,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":null,"pixel_height":null,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Each tomogram reconstruction is contained within its unique directory with an identifiable name. The folder called 'tomo_rec_4bin_SART' contains the 4 times binned volume reconstructed using aretomo as examples.","image_width":null,"image_height":null},{"segmentations":[],"name":"Raw frames from dataset 2 of microsporidian Encephalitozoon intestinalis","directory":"data/Data_Set_2","category":"tilt series","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":58,"frames_per_image":12,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":1.676,"pixel_height":1.676,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Each tilt series is contained within its unique directory with an identifiable name. The folder called 'raw_frames' contains the raw frames and corresponding mdoc field from each tilt series. These data were collected with super resolution.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Unaligned tilt stacks from dataset 2 of microsporidian Encephalitozoon intestinalis","directory":"data/Data_Set_2","category":"tilt series","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":58,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":3.353,"pixel_height":3.353,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Each tilt series is contained within its unique directory with an identifiable name. The folder called 'tilt_series' contains the unaligned tilt stacks with corresponding tilt files. The aligned tilt series file can be found in 'aligned_tilt_series'.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Reconstructed tomograms from dataset 2 of microsporidian Encephalitozoon intestinalis","directory":"data/Data_Set_2","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":58,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":null,"pixel_height":null,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Each tomogram reconstruction is contained within its unique directory with an identifiable name. The folder called 'tomo_rec_4bin_SART' contains the 4 times binned volume reconstructed using aretomo as examples.","image_width":null,"image_height":null}],"workflow_file":null,"grant_references":[{"funding_body":"American Heart Association","code":"915749","country":"United States"},{"funding_body":"Searle Scholars Program","code":"SSP-2018-2737","country":"United States"},{"funding_body":"National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)","code":"R01AI147131","country":"United States"},{"funding_body":"The Pew Charitable Trusts","code":"PEW-00033055","country":"United States"},{"funding_body":"NIH Common Fund Transformative High Resolution Cryo-Electron Microscopy program","code":"U24 GM129539","country":"United States"},{"funding_body":"Simons Foundation","code":"SF349247","country":"United States"},{"funding_body":"NY State Assembly","code":"N/A","country":"United States"},{"funding_body":"National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)","code":"9 P41 GM103310","country":"United States"},{"funding_body":"Irma T. Hirschl Career Scientist Award","code":"N/A","country":"United States"}],"version_history":[],"title":"Cryo-ET datasets (1 and 2) of dormant microporidian spores from Encephalitozoon intestinalis","principal_investigator":[{"author_orcid":"0000-0003-0624-6178","middle_name":null,"organization":"Department of Biology, Johns Hopkins University","street":null,"town_or_city":"Baltimore","state_or_province":"MD","post_or_zip":"21218","telephone":null,"fax":null,"first_name":"Gira","last_name":"Bhabha","email":"GBHABHA1 [at] JH.EDU","country":"United States","entry":"EMPIAR-12176"},{"author_orcid":"0000-0002-2570-0404","middle_name":"C","organization":"Department of Biology, Johns Hopkins University","street":null,"town_or_city":"Baltimore","state_or_province":"MD","post_or_zip":"21218","telephone":null,"fax":null,"first_name":"Damian","last_name":"Ekiert","email":"DEKIERT1 [at] JH.EDU","country":"United States","entry":"EMPIAR-12176"}],"status":"REL","deposition_date":"2024-06-11","release_date":"2025-05-22","obsolete_date":null,"update_date":"2025-05-22","corresponding_author":{"author":{"author_orcid":"0000-0002-9479-3800","middle_name":null,"organization":"New York University","street":null,"town_or_city":"New York","state_or_province":null,"post_or_zip":"10016","first_name":"Mahrukh","last_name":"Usmani","country":"United States"}},"authors":[{"author":{"name":"Usmani M","author_orcid":"0000-0002-9479-3800"}},{"author":{"name":"Coudray N","author_orcid":"0000-0002-6050-2219"}},{"author":{"name":"Raghu R","author_orcid":"0009-0006-6651-142X"}},{"author":{"name":"Ramchandani H","author_orcid":null}},{"author":{"name":"Bobe D","author_orcid":"0000-0002-7388-8907"}},{"author":{"name":"Kopylov M","author_orcid":"0000-0003-0188-9799"}},{"author":{"name":"Zhong ED","author_orcid":"0000-0001-6345-1907"}},{"author":{"name":"Ekiert DC","author_orcid":"0000-0002-2570-0404"}},{"author":{"name":"Bhabha G","author_orcid":"0000-0003-0624-6178"}}],"cross_references":["EMD-45673","EMD-45674"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Usmani M","author_orcid":"0000-0002-9479-3800"},{"name":"Coudray N","author_orcid":"0000-0002-6050-2219"},{"name":"Riggi M","author_orcid":"0000-0002-8930-5331"},{"name":"Raghu R","author_orcid":"0009-0006-6651-142X"},{"name":"Ramchandani H","author_orcid":null},{"name":"Bobe D","author_orcid":"0000-0002-7388-8907"},{"name":"Kopylov M","author_orcid":"0000-0003-0188-9799"},{"name":"Zhong ED","author_orcid":"0000-0001-6345-1907"},{"name":"Iwasa JH","author_orcid":"0000-0002-4949-7607"},{"name":"Ekiert DC","author_orcid":"0000-0002-2570-0404"},{"name":"Bhabha G","author_orcid":"0000-0003-0624-6178"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Cryo-ET reveals the in situ architecture of the polar tube invasion apparatus from microsporidian parasites","volume":"122","country":"","first_page":null,"last_page":null,"year":"2025","language":"English","doi":"10.1073/pnas.2415233122","pubmedid":"40067903","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Proceedings of the National Academy of Sciences of the United States of America","journal_abbreviation":"Proc Natl Acad Sci U S A","issue":"11","preprint":false}],"dataset_size":"1.3 TB","experiment_type":"EMDB","scale":"cell","entry_doi":"10.6019/EMPIAR-12176"}}