{"EMPIAR-12165":{"imagesets":[{"segmentations":[],"name":"Unalinged multi-frame micrographs of AceCas9 in super-resolution, dataset 1","directory":"data/Data_20210818_309306_slot2","category":"micrographs - multiframe","header_format":"MRC","data_format":"TIFF","num_images_or_tilt_series":5919,"frames_per_image":60,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.412,"pixel_height":0.412,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Gain file included as k3-gain-20210818.mrc.\nNo rotation, Flip upsidedown for motion import in RELION; no rotation, no flip in CryoSPARC.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Unalinged multi-frame micrographs of AceCas9 in super-resolution, dataset 2","directory":"data/Data_20211029_309306_AD2","category":"micrographs - multiframe","header_format":"MRC","data_format":"TIFF","num_images_or_tilt_series":7734,"frames_per_image":60,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.412,"pixel_height":0.412,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Gain file saved in dataset 1 folder as k3-gain-20210818.mrc.\nNo rotation, Flip upsidedown for motion import in RELION; no rotation, no flip in CryoSPARC","image_width":"11520","image_height":"8184"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health (NIH)","code":"R01GM101343","country":"United States"},{"funding_body":"National Institutes of Health (NIH)","code":"S10RR025080","country":"United States"},{"funding_body":"National Institutes of Health (NIH)","code":"U24GM116788","country":"United States"},{"funding_body":"National Institutes of Health (NIH)","code":"S10RR024564","country":"United States"},{"funding_body":"National Institutes of Health (NIH)","code":"S10 RR024564","country":"United States"},{"funding_body":"Department of Energy (DOE, United States)","code":"KP160711","country":"United States"}],"version_history":[],"title":"Cryo-EM structures of AceCas9","principal_investigator":[{"author_orcid":"0000-0003-2046-9861","middle_name":null,"organization":"Florida State University","street":"91 Chieftan Way","town_or_city":"Tallahassee","state_or_province":"FL","post_or_zip":"32306","telephone":"850-644-6785","fax":null,"first_name":"Hong","last_name":"Li","email":"hongli [at] sb.fsu.edu","country":"United States","entry":"EMPIAR-12165"}],"status":"REL","deposition_date":"2024-06-26","release_date":"2025-08-13","obsolete_date":null,"update_date":"2025-08-13","corresponding_author":{"author":{"author_orcid":"0000-0003-2046-9861","middle_name":null,"organization":"Florida State University","street":"91 Chieftan Way","town_or_city":"Tallahassee","state_or_province":"FL","post_or_zip":"32306","first_name":"Hong","last_name":"Li","country":"United States"}},"authors":[{"author":{"name":"Das A","author_orcid":null}},{"author":{"name":"Rai J","author_orcid":null}},{"author":{"name":"Roth MO","author_orcid":null}},{"author":{"name":"Shu Y","author_orcid":"0009-0000-8986-0725"}},{"author":{"name":"Medina ML","author_orcid":null}},{"author":{"name":"Barakat MR","author_orcid":null}},{"author":{"name":"Li H","author_orcid":"0000-0003-2046-9861"}}],"cross_references":["EMD-27146","EMD-27144","EMD-27142","EMD-27145","EMD-27143","EMD-27141"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Das A","author_orcid":null},{"name":"Rai J","author_orcid":null},{"name":"Roth MO","author_orcid":null},{"name":"Shu Y","author_orcid":"0009-0000-8986-0725"},{"name":"Medina ML","author_orcid":null},{"name":"Barakat MR","author_orcid":null},{"name":"Li H","author_orcid":"0000-0003-2046-9861"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Coupled catalytic states and the role of metal coordination in Cas9","volume":"6","country":"","first_page":"969","last_page":"977","year":"2023","language":"English","doi":"10.1038/s41929-023-01031-1","pubmedid":"38348449","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature catalysis","journal_abbreviation":"Nat Catal","issue":"10","preprint":false}],"dataset_size":"6.3 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["8d2q","8d2o","8d2l","8d2p","8d2n","8d2k"],"entry_doi":"10.6019/EMPIAR-12165"}}