{"EMPIAR-12149":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of spinach cytochrome b6f with DBMIB inhibitor bound at quinol oxidation site","directory":"data","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":3027,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.86,"pixel_height":0.86,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[{"funding_body":"Foundation for Polish Science","code":"POIR.04.04.00-00-5B54/17-00","country":"Poland"},{"funding_body":"Foundation for Polish Science","code":"TEAM TECH CORE FACILITY/2017-4/6","country":"Poland"},{"funding_body":"Polish National Science Centre","code":"2022/47/B/NZ1/03308","country":"Poland"}],"version_history":[],"title":"Single particle cryoEM data of spinach cytochrome b6f with inhibitor DBMIB bound in quinol oxidation site (Qp)","principal_investigator":[{"author_orcid":"0000-0002-1637-2365","middle_name":null,"organization":"Department of Molecular Biophysics; Faculty of Biochemistry, Biophysics and  Biotechnology; Jagiellonian University","street":"Gronostajowa 7","town_or_city":"Krakow","state_or_province":"Malopolska","post_or_zip":"30-387","telephone":null,"fax":null,"first_name":"Artur","last_name":"Osyczka","email":"artur.osyczka [at] uj.edu.pl","country":"Poland","entry":"EMPIAR-12149"},{"author_orcid":"0000-0003-2815-7133","middle_name":null,"organization":"Malopolska Centre of Biotechnology","street":"Gronostajowa 7A","town_or_city":"Krakow","state_or_province":"Malopolska","post_or_zip":"30-387","telephone":null,"fax":null,"first_name":"Sebastian","last_name":"Glatt","email":"sebastian.glatt [at] uj.edu.pl","country":"Poland","entry":"EMPIAR-12149"}],"status":"REL","deposition_date":"2024-06-24","release_date":"2025-06-05","obsolete_date":null,"update_date":"2025-06-05","corresponding_author":{"author":{"author_orcid":"0000-0001-8424-6590","middle_name":null,"organization":"Department of Molecular Biophysics; Faculty of Biochemistry, Biophysics and  Biotechnology; Jagiellonian University","street":"Gronostajowa 7","town_or_city":"Krakow","state_or_province":"Malopolska","post_or_zip":"30-387","first_name":"Rafal","last_name":"Pietras","country":"Poland"}},"authors":[{"author":{"name":"Pintscher S","author_orcid":"0000-0001-6356-2607"}},{"author":{"name":"Pietras R","author_orcid":"0000-0001-8424-6590"}},{"author":{"name":"Mielecki B","author_orcid":"0000-0002-8597-4888"}},{"author":{"name":"Indyka P","author_orcid":"0000-0002-9684-2127"}},{"author":{"name":"Rawski M","author_orcid":"0000-0002-8553-7637"}},{"author":{"name":"Koziej L","author_orcid":"0000-0003-3885-3845"}},{"author":{"name":"Jaciuk M","author_orcid":"0000-0003-1251-1492"}},{"author":{"name":"Wazny G","author_orcid":"0009-0006-6416-3097"}},{"author":{"name":"Glatt S","author_orcid":"0000-0003-2815-7133"}},{"author":{"name":"Osyczka A","author_orcid":"0000-0002-1637-2365"}}],"cross_references":["EMD-19940"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Pintscher S","author_orcid":"0000-0001-6356-2607"},{"name":"Pietras R","author_orcid":"0000-0001-8424-6590"},{"name":"Mielecki B","author_orcid":"0000-0002-8597-4888"},{"name":"Szwalec M","author_orcid":"0000-0002-3548-2164"},{"name":"Wójcik-Augustyn A","author_orcid":"0000-0002-3053-093X"},{"name":"Indyka P","author_orcid":"0000-0002-9684-2127"},{"name":"Rawski M","author_orcid":"0000-0002-8553-7637"},{"name":"Koziej Ł","author_orcid":"0000-0003-3885-3845"},{"name":"Jaciuk M","author_orcid":"0000-0003-1251-1492"},{"name":"Ważny G","author_orcid":null},{"name":"Glatt S","author_orcid":"0000-0003-2815-7133"},{"name":"Osyczka A","author_orcid":"0000-0002-1637-2365"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Molecular basis of plastoquinone reduction in plant cytochrome b<sub>6</sub>f","volume":"10","country":"","first_page":"1814","last_page":"1825","year":"2024","language":"English","doi":"10.1038/s41477-024-01804-x","pubmedid":"39362993","details":"We present cryo-EM structures of spinach cytb6f at 1.9 and 2.2 Å resolution, revealing an unexpected orientation of the substrate PQ in the haem ligand niche that forms the PQ reduction site, and a structure of cytb6f with DBMIB inhibitor bound in Qp site.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature plants","journal_abbreviation":"Nat Plants","issue":"11","preprint":false}],"dataset_size":"1.1 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9es9"],"entry_doi":"10.6019/EMPIAR-12149"}}