{"EMPIAR-12124":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of acetyl-CoA decarbonylase/synthase (ACDS) complex and subcomplexes","directory":"data/Images-Disc1","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":13892,"frames_per_image":40,"frame_range_min":0,"frame_range_max":39,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":0.654,"pixel_height":0.654,"micrographs_file_pattern":"data/Images-Disc1/GridSquare_*/Data/FoilHole_*_fractions.tiff","picked_particles_file_pattern":"data/particle_coords/run_data_*_selection.star","picked_particles_directory":"data/particle_coords","details":"Gain reference: \ndata/GainsBackup2022-12-13/K3-18030019GainRef.x1.m1.bin2.mrc\nDefocus range: 0.0 to -1.75 µM\nTotal exposure on specimen: 47.1 el/A2\nExposure time: 1.09 s\nCalibrated pixel size: 0.654 (0.327 A super res)\nObjective lens spherical aberration (Cs): 2.7 mm\nAcceleration voltage: 300 kV\nExtraction voltage: 3550 V\nMicroscope / Detector: Krios G3i / K3\n\nPicked particles coordinates include:\nrun_data_initial_selection.star : An initial selection including a mixture of carbon monoxide dehydrogenase (CODH) alone and CODH in complex with acetyl-CoA synthase (ACS)\nrun_data_tetramer_selection.star : A final selection of tetramer CODH alone \nrun_data_pentamer_selection.star : A final selection of tetramer CODH with one ACS bound\nrun_data_hexamer_selection.star : A final selection of tetramer CODH with two ACSs bound","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[{"funding_body":"Howard Hughes Medical Institute (HHMI)","code":"N/A","country":"United States"},{"funding_body":"National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)","code":"GM126982","country":"United States"}],"version_history":[],"title":"Cryogenic electron microscopy of chameleon-plunged acetyl-CoA decarbonylase/synthase","principal_investigator":[{"author_orcid":"0000-0001-5486-2755","middle_name":"L","organization":"Departments of Chemistry and Biology, Massachusetts Institute of Technology","street":"31 Ames St","town_or_city":"Cambridge","state_or_province":"MA","post_or_zip":"02142","telephone":null,"fax":null,"first_name":"Catherine","last_name":"Drennan","email":"cdrennan [at] mit.edu","country":"United States","entry":"EMPIAR-12124"}],"status":"REL","deposition_date":"2024-05-31","release_date":"2025-02-03","obsolete_date":null,"update_date":"2025-02-03","corresponding_author":{"author":{"author_orcid":"0000-0001-5486-2755","middle_name":"L","organization":"Departments of Chemistry and Biology, Massachusetts Institute of Technology","street":"31 Ames St","town_or_city":"Cambridge","state_or_province":"MA","post_or_zip":"02142","first_name":"Catherine","last_name":"Drennan","country":"United States"}},"authors":[{"author":{"name":"Biester A","author_orcid":"0000-0003-4129-1871"}},{"author":{"name":"Drennan CL","author_orcid":"0000-0001-5486-2755"}}],"cross_references":["EMD-45090","EMD-45092","EMD-45091"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Biester A","author_orcid":"0000-0003-4129-1871"},{"name":"Grahame DA","author_orcid":"0000-0002-8292-4690"},{"name":"Drennan CL","author_orcid":"0000-0001-5486-2755"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Capturing a methanogenic carbon monoxide dehydrogenase/acetyl-CoA synthase complex via cryogenic electron microscopy","volume":"121","country":"","first_page":null,"last_page":null,"year":"2024","language":"English","doi":"10.1073/pnas.2410995121","pubmedid":"39361653","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Proceedings of the National Academy of Sciences of the United States of America","journal_abbreviation":"Proc Natl Acad Sci U S A","issue":"41","preprint":false}],"dataset_size":"1.8 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9c0r","9c0t","9c0s"],"entry_doi":"10.6019/EMPIAR-12124"}}