{"EMPIAR-12110":{"imagesets":[{"segmentations":[],"name":"Unaligned micrographs of tilt series collected from Jurkat cells capturing extracellular intermediate filaments.","directory":"data/EMD-43978/frames","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":183,"frames_per_image":10,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":3.465,"pixel_height":3.465,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned micrographs of tilts series collected from Jurkat cells capturing extracellular intermediate filaments collected on a 300kV Titan Krios.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Gain reference for the unaligned micrographs of tilt series collected from Jurkat cells capturing extracellular intermediate filaments.","directory":"data/EMD-43978/gainref","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":3.465,"pixel_height":3.465,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Tilt series collected from Jurkat cells capturing extracellular intermediate filaments.","directory":"data/EMD-43978/tiltseries","category":"tilt series","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":3,"frames_per_image":61,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":3.465,"pixel_height":3.465,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Reconstructed tomograms (unbinned) generated from tilt series collected from Jurkat cells capturing extracellular intermediate filaments.","directory":"data/EMD-43978/tomograms/unbinned","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":3,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED BYTE","pixel_width":3.465,"pixel_height":3.465,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"","image_width":"4092","image_height":"5760"},{"segmentations":[],"name":"Reconstructed tomograms (bin 4) generated from tilt series collected from Jurkat cells capturing extracellular intermediate filaments.","directory":"data/EMD-43978/tomograms/bin4","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":3,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED BYTE","pixel_width":13.86,"pixel_height":13.86,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"","image_width":"1023","image_height":"1440"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)","code":"AI20459","country":"United States"},{"funding_body":"National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)","code":"AI159375","country":"United States"},{"funding_body":"National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)","code":"GM130332","country":"United States"}],"version_history":[],"title":"Extracellular filaments revealed by affinity capture cryo-electron tomography of lymphocytes","principal_investigator":[{"author_orcid":"0000-0002-4614-7310","middle_name":"Lyne","organization":"Stanford University","street":"240 Pasteur Drive","town_or_city":"Stanford","state_or_province":"California","post_or_zip":"94305","telephone":null,"fax":null,"first_name":"Stefan","last_name":"Oliver","email":"sloliver [at] stanford.edu","country":"United States","entry":"EMPIAR-12110"}],"status":"REL","deposition_date":"2024-05-13","release_date":"2026-02-23","obsolete_date":null,"update_date":"2026-02-23","corresponding_author":{"author":{"author_orcid":"0000-0002-4614-7310","middle_name":"Lyne","organization":"Stanford University","street":"240 Pasteur Drive","town_or_city":"Stanford","state_or_province":"California","post_or_zip":"94305","first_name":"Stefan","last_name":"Oliver","country":"United States"}},"authors":[{"author":{"name":"Engel L","author_orcid":"0000-0002-5329-4300"}},{"author":{"name":"Zaoralová M","author_orcid":null}},{"author":{"name":"Zhou M","author_orcid":null}},{"author":{"name":"Dunn AR","author_orcid":"0000-0001-6096-4600"}},{"author":{"name":"Oliver SL","author_orcid":"0000-0002-4614-7310"}}],"cross_references":[],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Engel L","author_orcid":"0000-0002-5329-4300"},{"name":"Zaoralová M","author_orcid":null},{"name":"Zhou M","author_orcid":null},{"name":"Dunn AR","author_orcid":null},{"name":"Oliver SL","author_orcid":"0000-0002-4614-7310"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Extracellular filaments revealed by affinity capture cryo-electron tomography of lymphocytes","volume":null,"country":"","first_page":null,"last_page":null,"year":"2023","language":null,"doi":"10.1101/2023.08.05.552110","pubmedid":null,"details":"This deposition contains the raw movie files, gain reference, and mdoc files to generate tiltseries for tomogram reconstruction.  Tiltseries generated by WARP are included for the reconstruction of tomograms in IMOD.  Reconstructed tomograms, unbinned and binned x4, are also included.  The raw movie files and gain reference were used to generate the cryo-EM map available for deposition EMD-43978.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"bioRxiv","journal_abbreviation":"","issue":null,"preprint":true}],"dataset_size":"75.6 GB","experiment_type":"FIB-SEM","scale":"molecule","entry_doi":"10.6019/EMPIAR-12110"}}